تأثیر 8 هفته تمرین تناوبی خیلی شدید بر بیان ژن عوامل تنظیمی عضله زایی MyF5 و میوژنین در عضلۀ دوقلوی موشهای صحرایی

بیگلری, سهیل; گائینی, عباسعلی

doi:10.48308/joeppa.2019.98928

تأثیر 8 هفته تمرین تناوبی خیلی شدید بر بیان ژن عوامل تنظیمی عضله زایی MyF5 و میوژنین در عضلۀ دوقلوی موشهای صحرایی

نوع مقاله : علمی - پژوهشی

نویسندگان

دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران

10.48308/joeppa.2019.98928

چکیده

هدف: باوجود نقش مهم فرایند عضله زایی در بازسازی و رشد عضلۀ اسکلتی، تأثیر تمرین های تناوبی خیلی شدید ) HIIT ( بر عوامل
آن مشخص نیست. هدف از پژوهش حاضر، بررسی تأثیر هشت هفته HIIT بر عوامل مهم عضله زایی )عامل عضله زایی 5 ( MyF5 )
و میوژنین( در عضلۀ دوقلوی موش های صحرایی است.
روش ها: در این مطالعه، 16 موش صحرایی نر نژاد ویستار با سن 10 هفته و محدودۀ وزنی 25 ± 225 گرم، به صورت تصادفی به
دو گروه کنترل ) 8 = n ( و HIIT ( 8 = n ( تقسیم شدند. برنامۀ تمرین HIIT به مدت 40 دقیقه، سه روز در هفته و به مدت هشت هفته
اجرا شد. 48 ساعت پس از آخرین جلسۀ تمرینی، عضلۀ دوقلو استخراج و میزان بیان ژن MyF5 و میوژنین به روش Real time-PCR سنجیده شد. به منظور تجزیه وتحلیل آماری داده ها، از آزمون t مستقل استفاده شد.
نتایج: بیان ژن MyF5 و میوژنین در گروه HIIT در مقایسه با گروه کنترل افزایش معناداری ) 05 / 0p˂ ( داشت. همچنین، وزن عضلۀ
دوقلو در گروه تمرینی در مقایسه با گروه کنترل به طور معنادار بالاتر بود ) 01 / 0p˂ .)
نتیجه گیری: به نظر میرسد، HIIT منجر به افزایش بارز بیان ژن MyF5 ، میوژنین و وزن عضلۀ دوقلوی موش های صحرایی میشود.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of eight weeks of High-intensity Interval Training on the gene expression of the myogenic regulatory factors MyF5 and myogenin in the skeletal muscle of rats

نویسندگان [English]

Soheil Biglari
Abbas Ali Gaeini

Department of Sport Physiology, Faculty of Physical Education and Sports Sciences, University of Tehran, Tehran, Iran

چکیده [English]

Purpose: Myogenesis plays an important role in the skeletal muscle reconstruction and growth. However, the impact of High-intensity Interval Training (HIIT) on the factors of this process is unknown. The purpose of this study was to investigate the effect of 8 weeks HIIT on myogenesis main factors, i.e. Myogenic Factor 5
)Myf5( and Myogenin in the rat's gastrocnemius muscle.
Methods: In this experimental study, sixteen male Wistar rats (at age of 10 weeks, weight= 255 ± 25 gr) were randomly divided into two groups (control (n = 8) and HIIT (n = 8)). HIIT protocol was performed for 40 minutes, three days a week for eight weeks. 48 hours after the last training session, gastrocnemius muscle was extracted and MyF5 and myogenin gene expression were measured by Real Time-PCR. To analyze the data, independent T-test was used.
Results: Compared to the control group, MyF5 and myogenin gene expression significantly increased in the experimental group (P˂0.05). Additionally, gastrocnemius muscle weight in training group was significantly higher than the control group (P˂0.01).
Conclusion: It seems that HIIT leads to a significant increase in the expression of MyF5, Myogenin, and gastrocnemius muscle weight in rats.

کلیدواژه‌ها [English]

HIIT
MyF5
Myogenesis
Myogenin
Real time-PCR

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