نشریه فیزیولوژی ورزش و فعالیت بدنی

نشریه فیزیولوژی ورزش و فعالیت بدنی

تأثیر هشت هفته تمرین تداومی همراه با دریافت امگا 3 بر بیان برخی ناقلان اسید صفراوی و میزان اسیدهای صفراوی در بافت کبد موش‌های نر صحرایی با الگوی کبد چرب غیرالکلی القایی

نوع مقاله : مقاله پژوهشی

نویسندگان
1 گروه فیزیولوژی ورزشی، دانشکدة علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز، ایران
2 گروه علوم پایه، دانشکدة دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران
چکیده
زمینه و هدف؛ بیماری کبد چرب غیرالکلی (NAFLD) یکی از شایع‌ترین اختلالات سوخت‌وسازی است که با انباشت چربی، التهاب و اختلال در سوخت‌وساز اسیدهای صفراوی همراه است. تغییر در بیان ناقلان اسید صفراوی کبدی نقش مهم و ارزنده‌ای در پیشرفت این بیماری دارد. از این‌رو، این پژوهش با هدف بررسی تأثیر هشت هفته تمرین تداومی با شدت متوسط همراه با دریافت مکمل امگا-۳ بر بیان ناقلان اسید صفراوی و میزان اسیدهای صفراوی در بافت کبد موش‌های مبتلا به NAFLD انجام گرفت.

مواد و روش‌ها: 30 سر موش صحرایی نر بالغ ویستار به‌طور تصادفی به پنج گروه کنترل سالم، کبد چرب غیرالکلی (NAFLD)،NAFLD همراه با تمرین تداومی با شدت متوسط (MICT)،NAFLD همراه با مکمل امگا-۳ (OM3) و NAFLD همراه با تمرین تداومی و امگا-۳ جایگزین شدند. القای NAFLD با رژیم غذایی پرچرب و دریافت محلول سوکروز 25 درصد به مدت 12 هفته انجام گرفت. تمرین تداومی به مدت هشت هفته، چهار جلسه در هفته اجرا شد. متغیرهای پژوهش شامل بیان ناقلان اسیدهای صفراوی کبدی، مانند پلی‌پپتید ناقل هم انتقال‌دهندة سدیم–توروکولات (NTCP) و پمپ خروج نمک صفراوی BSEP))، غلظت اسیدهای صفراوی، قند خون و نیمرخ لیپیدی بود. داده‌ها با استفاده از آزمون تحلیل واریانس یکطرفه و آزمون تعقیبی توکی در سطح معناداری 05/0>P تجزیه‌وتحلیل شدند.

نتایج:NAFLD موجب افزایش اسیدهای صفراوی، اختلال نیمرخ سوخت‌وسازی و کاهش بیان ناقلان صفراوی شد (05/0>P). تمرین تداومی و مصرف امگا-۳ به‌طور مستقل و جداگانه سبب بهبود نشانگرهای سوخت‌وسازی، کاهش اسیدهای صفراوی و تعدیل بیان NTCP و BSEP شدند. بیشترین تأثیرات مطلوب در گروه ترکیبی دیده شد (05/0>P).

نتیجه‌گیری: نتایج نشان داد که ترکیب تمرین تداومی و مکمل امگا-۳ احتمالاً می‌تواند با تنظیم هومئوستاز اسیدهای صفراوی و بهبود عملکرد کبد به‌عنوان راهبردی غیردارویی و مؤثر در مدیریت NAFLD مطرح شود.
کلیدواژه‌ها
موضوعات

عنوان مقاله English

The effect of 8 weeks of continuous exercise combined with omega-3 consumption on the expression of bile acid transporters and the amount of bile acids in the liver tissue of male rats with an induced non-alcoholic fatty liver disease model

نویسندگان English

Hamid Mohammadi 1
Rouhollah Ranjbar 1
Mohammad reza Tabandeh 2
Abdolhamid Habibi 1
1 Department of Sport Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 Department of Basic Science, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
چکیده English

Background and Purpose: Nonalcoholic fatty liver disease (NAFLD) is one of the most common metabolic disorders associated with fat accumulation, inflammation, and impaired bile acid metabolism. Alterations in the expression of hepatic bile acid transporters play an important role in the progression of this disease. The aim of the present study was to investigate the effect of eight weeks of moderate-intensity continuous exercise combined with omega-3 supplementation on the expression of bile acid transporters and bile acid levels in the liver tissue of rats with NAFLD.

Materials and Methods: Thirty adult male Wistar rats were randomly divided into five groups: healthy control, nonalcoholic fatty liver disease (NAFLD), NAFLD with moderate-intensity continuous exercise (MICT), NAFLD with omega-3 supplementation (OM3), and NAFLD with continuous exercise and omega-3. NAFLD was induced with a high-fat diet and 25% sucrose solution for 12 weeks. The continuous exercise protocol was performed four sessions per week for 8 weeks. The expression of hepatic bile acid transporters including sodium-taurocholate cotransporting polypeptide (NTCP) and bile salt export pump (BSEP), bile acid concentrations, blood glucose, and lipid profile were measured following all protocols. Data were analyzed using one-way ANOVA and Tukey's post-hoc test.

Results: NAFLD increased bile acids, impaired metabolic profile, and decreased expression of bile transporters significantly (P

کلیدواژه‌ها English

Non-Alcoholic Fatty Liver Disease
Continuous training
Omega-3
Bile acid transporter
Liver
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