اثر تمرین هوازی و مقاومتی بر میزان شاخص مقاومت به انسولین و نسبت BCL-2/BAX در مسیر آپوپتوزی بافت قلب موش‌های صحرایی دیابتی نر نژاد ویستار

نوع مقاله : علمی - پژوهشی

نویسندگان

دانشکدۀ تربیت بدنی و علوم ورزشی، دانشگاه تربیت دبیر شهید رجایی، تهران، ایران

چکیده

هدف: دیابت، اختلال متابولیکی است که از طریق هایپرگلایسمی مشخص می‌شود و نقش مهمی در ایجاد آپوپتوز قلبی دارد. شواهد حاکی از آن است که تمرینات ورزشی می‌توانند برخی از مسیرهای پیام‌رسانی مربوط به آپوپتوز را تحت تأثیر قرار دهند. هدف از پژوهش حاضر مقایسۀ اثر تمرین هوازی و مقاومتی بر میزان شاخص مقاومت به انسولین و نسبت BCL-2 به BAX بر موش‌های صحرایی دیابتی نر نژاد ویستار است.
روش‌ها: در این تحقیق تجربی، 25 سر موش صحرایی نر نژاد ویستار 200 تا 250 گرمی به‌طور تصادفی به پنج گروه: تمرین هوازی (6n=)، تمرین مقاومتی (6n=)، شم (6n=)، کنترل (4n=) و سالم (3n=) تقسیم شدند. موش‌های صحرایی با مصرف رژیم غذایی پرچرب به مدت شش هفته و پس از شش هفته تزریق تک‌دوز استرپتوزوتوسین به مقدار 30 میلی‌گرم بر کیلوگرم دیابتی شدند. موش‌های صحرایی گروه هوازی به مدت چهار هفته و هفته‌ای پنج جلسه روی نوار گردان و موش‌های صحرایی گروه مقاومتی به مدت چهار هفته و هفته‌ای شش جلسه با استفاده از نردبان به تمرین پرداختند.
نتایج: تفاوت میزان پروتئین BAX گروه هوازی و مقاومتی معنادار و مقدار آن در گروه مقاومتی نسبت به هوازی کمتر بود (014/0=P). تفاوت نسبت BCL-2 به BAX گروه هوازی و مقاومتی معنادار و در گروه مقاومتی بیشتر از گروه هوازی بود (05/0=P). همچنین میزان شاخص مقاومت به انسولین در گروه هوازی (005/0=P) و در گروه مقاومتی (004/0=P) پس از تمرین کاهش بیشتری نسبت به قبل تمرین داشت و این اختلاف معنادار بود.
نتیجه‌گیری: به‌نظر می‌رسد هم تمرین مقاومتی و هم تمرین هوازی در کاهش میزان شاخص مقاومت به انسولین اثرگذارند و در مقایسۀ بین دو تمرین هوازی و مقاومتی، تمرین مقاومتی بیشتر در کاهش عوامل آپوپتوزی اثر بیشتری دارد و با افزایش شدت تمرین، میزان SIRT1 افزایش می‌یابد و سبب مهار عوامل آپوپتوزی می‌شود.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of aerobic and resistance training on insulin resistance index (HOMA-IR) and BCL-2/BAX ratio in apoptotic pathway in the heart tissue of male wistar diabetic rats

نویسندگان [English]

  • Fatemeh Nourzad
  • Fereshteh Shahidi
  • Mojtaba Saleh pourpour
Faculty of Physical Education and Sports Sciences, Tarbiat Dabir Shahid Rajaei University, Tehran, Iran
چکیده [English]

Purpose: Diabetes is a metabolic disorder characterized by hyperglycemia and plays an important role in the development of cardiac apoptosis. Evidence suggests that exercise can affect some of the signaling pathways associated with apoptosis. Evidence suggests that exercise can affect some of the apoptosis-related signaling pathways. The aim of this study was to compare the effect of aerobic and resistance training on insulin resistance index and BCL-2 to BAX ratio in male Wistar diabetic rats.
Methods: In this experimental study, 25 male Wistar rats in the weight range of 200 to 250 g were randomly divided into five groups: Aerobic training (n = 6), Resistance training (n = 6), Sham (n = 6), Control (n = 4), Healthy (n = 3) Rats became diabetic by consuming a high-fat diet for six weeks and after six weeks with a single dose of 30 mg / kg streptozotocin injection. Rats in the aerobic group trained on a treadmill for four weeks and five sessions per week, and rats in the resistance group trained on a ladder for four weeks and six sessions per week.
Results: The results showed that there was a significant difference between the aerobic and resistance groups of BAX protein and the amount of this protein in the resistance group was lower than aerobic (P= 0.014). There was a significant difference between BCL-2 and BAX ratio between aerobic and resistance groups and this ratio was higher in the resistance group than the aerobic group (P = 0.05). Also, the rate of insulin resistance index in the aerobic group (P = 0.005) and in the resistance group (P = 0.004) after exercise decreased more than before exercise and this difference was significant.
Conclusion: It seems that both resistance training and aerobic training are effective in reducing the amount of insulin resistance index and in comparison between aerobic and resistance training, more resistance training is more effective in reducing apoptotic factors and with increasing exercise intensity, SIRT1 is increased and it inhibits apoptotic factors.

کلیدواژه‌ها [English]

  • BCL-2
  • BAX
  • aerobic training
  • resistance training

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  • تاریخ دریافت: 19 مهر 1399
  • تاریخ بازنگری: 01 اردیبهشت 1400
  • تاریخ پذیرش: 27 اردیبهشت 1400
  • تاریخ اولین انتشار: 19 دی 1400
  • تاریخ انتشار: 01 فروردین 1401

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