تفاوت سنجی بیان ژن میوستاتین در عضلات کند و تند انقباض موش‌های صحرایی نر پس از 8 هفته تمرین تناوبی خیلی شدید

نوع مقاله : علمی - پژوهشی

نویسندگان

1 دانشگاه تهران، دانشکده تربیت بدنی و علوم وزشی

2 دانشگاه تهران، دانشکده تربیت بدنی و علوم ورزشی

چکیده

هدف از پژوهش حاضر، سنجش تفاوت بیان ژن میوستاتین در عضلات کند و تند انقباض رت‌های نر سالم پس از 8 هفته تمرین تناوبی خیلی شدید بود. روش تحقیق: به همین منظور، 12 سر رت نر ویستار به دو گروه کنترل(6=n) و تمرین (6=n) تقسیم شدند. تمرین تناوبی خیلی شدید شامل پنج روز در هفته و به مدت هشت هفته بود. هر جلسه از سه تناوب (چهار دقیقه ای با شدت 90 تا 100 درصدVO2max و دو دقیقه ای با شدت 50 تا 60 درصد VO2max) تشکیل می‌شد. 24 ساعت پس از آخرین جلسه‌ تمرین، عضله باز کننده طویل انگشتان و نعلی استخراج و میزان بیان ژن آنها با روش RT-PCR سنجیده شد. نتایج: تحلیل آماری با آزمون t  مستقل نشان داد که مقادیر میوستاتین عضله نعلی و بازکننده طویل انگشتان در گروه تجربی در مقایسه با گروه کنترل کاهش معناداری داشت (001/0 = p ). نتیجه گیری: نتایج تحقیق نشان داد که با استفاده از اجرای HIIT بیان میوستاتین در عضله EDL کاهشی چشمگیر (30% ) و در عضله نعلی کاهشی اندک ( 7% ) داشته است. در نتیجه پس از اجرای این پروتکل، بیان میوستاتین در عضلات تندانقباض نسبت به عضلات کندانقباض بیشتر سرکوب شده است.

عنوان مقاله [English]

The difference of myostatin gene expression in fast and slow twitch healthy male rat after eight weeks of high intensity interval training

چکیده [English]

The difference of myostatin gene expression in fast and slow twitch healthy male rat after eight weeks of high intensity interval training
The aim of the present study was to assess differences in myostatin gene expression in slow and fast-twitch
muscles of healthy male rats after 8 weeks high intensity interval training. Methods: For this purpose, 12 male
Wistar rats divided into two groups, control (6 = n) and trained (6 = n). High intensity interval training was
five days a week for eight weeks. Each session was formed from three intervals (four-minute with intensity of
90 to 100% VO2max and two- minute with intensity of 50 to 60% VO2max). 24 hours after the last training
session, the Extensor digitorum longus and soleus muscle were extracted and gene expression levels were
measured by RT-PCR. Result: Statistical analysis using independent t-test showed that the soleus and EDL
muscles myostatin levels decreased significantly in the experimental group compared with the control group
(0/001 = p). Conclusion: Hence it can be concluded that myostatin expression in both EDL and soleus muscle
is reduced by high intensity interval training.

کلیدواژه‌ها [English]

  • myostatin
  • High Intensity Interval Training
  • soleus muscle
  • Extensor digitorum longus muscle
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  • تاریخ دریافت: 23 شهریور 1395
  • تاریخ بازنگری: 22 خرداد 1403
  • تاریخ پذیرش: 11 دی 1399
  • تاریخ اولین انتشار: 11 دی 1399
  • تاریخ انتشار: 01 خرداد 1395