نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشکده علوم ورزش، دانشگاه بوعلی سینا همدان، همدان، ایران
2 اداره آموزش و پرورش اسلام آباد غرب، معاونت تربیت بدنی و سلامت،اسلام آباد غرب، ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Background and Purpose: Obesity is a chronic metabolic disorder that is usually caused by long-term positive energy balance during human life, is associated with increased adipose tissue mass and leads to skeletal abnormalities, physiological disorders and functional deficits over time. Obesity increases the risk of progression of other chronic diseases and is usually associated with premature death. Obesity increases the risk of various types of diseases, including conditions that cause the development of more than 200 chronic diseases such as cardiovascular disease, cancer, cerebrovascular diseases, type 2 diabetes, hypertension, asthma, mental illness, polycystic ovary syndrome, non-alcoholic fatty liver disease, gastrointestinal reflux, gallbladder disease, rheumatoid arthritis In general, obesity directly and indirectly reduces the quality of life and imposes a lot of economic costs on the society. Obesity is associated with interruption in glucose metabolism and insulin function. Therefore, the aim of this study was to investigate the effect of exercise training alone and in combination with creatine supplementation on glucose homeostasis, insulin efficiency and muscle glycogen regulator proteins in high fat died-induced obese rat.
Materials and Methods: 50 male Wistar rats (17±170 g) were divided into two groups of 10 control (standard food) and 40 high-fat diet (HFD). After 12 weeks after induction of obesity, 10 control rats and 10 rats from HFD group were killed. Then, 30 obese rats were randomly divided into three groups: control obese, swimming training and swimming training plus creatine supplementation. Finally, rats underwent oral glucose tolerance test (OGTT) and blood samples were taken to measure glucose and insulin levels. Also, the right SOL muscle was removed to measure the protein level of GLUT4 and glycogen synthase. For evaluation of protein levels of samples, Western blot technique, and for analyzing data, one-way ANOVA been used. P value for statistical differences set as 0.05
Results: After 12 weeks of HFD consumption, obesity decreased glucose tolerance and insulin efficiency and increased fasting insulin and glucose in rats. Also, obesity reduced the protein levels of GLUT4 and muscle glycogen synthase. Ten weeks of training increased glucose tolerance, insulin efficiency and increased protein levels of GLUT4 and glycogen synthase. Compared with training alone, creatine supplementation combined with training synergistically increased glucose tolerance, insulin efficiency and muscle GLUT4 and GS protein levels (P<0.05).
Conclusion: Creatine supplementation combination with swimming exercises increased the glucose metabolism and this effect is probably due to higher muscle glucose uptake and increased levels of GLUT4 and glycogen synthase proteins expression in skeletal muscles.
کلیدواژهها [English]
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