اثر 12 هفته تمرین شنا و دریافت ژل رویال بر بیان ژن⁠های JNK و AP-1 بافت ریه در موش‌های مبتلا به سرطان ریه

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم ورزشی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران

2 گروه علوم ورزشی، واحد مرودشت، دانشگاه آزاد اسلامی، مرودشت، ایران

چکیده

زمینه و هدف: سرطان ریه از مهم⁠ترین عوامل مرگ‌ومیر جهانی در حوزۀ سلامت عمومی است. فشار اکسایشی و عوامل التهابی نقش مهمی در بروز و توسعة سرطان ریه دارند. گمان می‌رود فعالیت ورزشی همراه با دریافت مکمل⁠های ضد التهابی بتواند با کاهش فشار اکسایشی و عوامل التهابی در بهبود سرطان ریه مؤثر باشد، هرچند این موضوع به‌درستی مشخص نیست. از این‌رو، این پژوهش با هدف بررسی اثر 12 هفته تمرین شنا و دریافت ژل رویال بر بیان ژن⁠های JNK و پروتئین فعال‌کننده 1 (AP-1) در موش‌های مبتلا به سرطان ریه با بنزوپیرن انجام گرفت.
مواد و روش‌ها: در این پژوهش تجربی و بنیادی، 36 سر موش نر نژاد Balb/c با سن هشت تا 10 هفته و وزن 18 تا 25 گرم به‌طور تصادفی به شش گروه کنترل سالم (6=n)، شم (6=n) و آزمایش (24=n) تقسیم شدند. سپس موش‌های گروه آزمایش پس از القای سرطان با تزریق 100 میلی⁠گرم به ازای هر کیلوگرم وزن بدن بنزوپیرن، به چهار زیرگروه شامل سرطان ریه (6=n)، تمرین شنا (6=n)، ژل رویال (6=n) و تمرین + ژل رویال (6=n) تقسیم شدند. تمرین شنا به‌صورت پیش‌رونده، سه بار در هفته و به مدت 12 هفته اجرا شد. روزانه به مدت 12 هفته به ازای هر کیلوگرم وزن بدن، 100 میلی‌گرم ژل رویال به‌صورت داخل‌صفاقی تزریق شد. 48 ساعت پس از آخرین جلسة تمرین، بیان ژن⁠های JNK و AP-1 با استفاده از روش Real-Time PCR در بافت ریه سنجیده شد. داده‌ها با نرم‌افزار SPSS نسخة 22 و آزمون⁠ آماری تحلیل واریانس یکراهه همراه با آزمون تعقیبی توکی و آزمون⁠ آماری تحلیل واریانس دوراهه همراه با آزمون تعقیبی بونفرونی تجزیه‌وتحلیل شدند.
نتایج: یافته‌های این پژوهش نشان داد بیان ژن⁠های  JNKو AP-1 در بافت ریه موش‌های گروه سرطان ریه نسبت به گروه کنترل سالم افزایش معناداری داشت (001/0=P). همچنین یافته‌ها نشان داد اگرچه دریافت ژل رویال موجب کاهش بیان ژن JNK و AP-1 در مقایسه با گروه سرطان ریه می‌شود (به‌ترتیب 044/0=P و 007/0=P)، اما تفاوت بیان این دو ژن در گروه تمرین شنا نسبت به گروه سرطان ریه معنادار نبود (به‌ترتیب 056/0=P و 18/0=P). روی‌هم‌رفته یافته‌ها نشان داد تمرین+ ژل رویال بیشترین کاهش را در بیان ژن⁠های JNK و AP-1 نسبت به گروه سرطان داشت (هر دو 001/0=P).
نتیجه‏گیری: با توجه به یافته‌های این پژوهش گمان می‌رود تمرین شنا در کنار دریافت ژل رویال، تأثیرات مهاری و محافظتی بیشتری در بیان ژن⁠های JNK و AP-1 بافت ریه در مقایسه با هر کدام از این مداخله⁠گرها به‌تنهایی در نمونه⁠های مبتلا به سرطان ریه دارد. برای تعمیم نتایج به نمونه⁠های انسانی نیازمند پژوهش‌های بیشتری به‌ویژه از نوع کاربردی است

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effects of 12 weeks of swimming training and royal jelly consumption on the expression of JNK and AP-1 genes of lung tissue in mice with lung cancer

نویسندگان [English]

  • Hossein Rasoulizadeh 1
  • Mehrzad Moghadasi 1
  • Seyed Ali Hosseini Hosseini 2
1 Department of sport sciences, Shi.C., Islamic Azad University, Shiraz, Iran
2 Department of sport sciences, Marv.C., Islamic Azad University, Marvdasht, Iran
چکیده [English]

Background and Purpose: Lung cancer is one of the most important causes of global mortality and oxidative stress and inflammatory factors play an important role in its occurrence and development. Probably regular exercise along with taking anti-inflammatory supplements can be effective in improving lung cancer through reducing oxidative stress and inflammatory factors, though, this is not well known and needs to be further investigated. Therefore, we aimed to investigate the effect of 12 weeks of swimming training and royal jelly consumption on the expression of JNK and activator protein 1 (AP-1) genes in mice with Benzo[a]pyrene -induced lung cancer.
Materials and Methods: In this experimental study, 36 male Balb/c mice (age, 8 to 10 weeks; weight, 18 to 25 g) were randomly assigned to 6 groups: healthy control (n=6), sham (n=6), and experimental (n=24). Thereafter, cancer was induced by injection of 100 mg/kg body weight of benzopyrene, and the mice in the experimental group were divided into four subgroups of control with lung cancer (n=6), swimming training (n=6), royal jelly (n=6), and training + royal jelly (n=6). Swimming training was performed three times a week for 12 weeks. Royal jelly was also injected intraperitoneally at a dose of 100 mg/kg of body weight daily for 12 weeks. Forty-eight hours after the last training session, JNK and AP-1 gene expression were measured using Real-Time PCR. To analyze the data, a one-way analysis of variance (ANOVA) test with Tukey post-hoc tests and two-way ANOVA with Bonferroni post-hoc tests were used.
Results: Data analyses revealed that the expression of JNK and AP-1 genes in the lung cancer group was significantly increased compared to the healthy control group (p=0.001). Although the expression of JNK and AP-1genes were lower in the royal jelly group compared to the control cancer group (p=0.044, p=0.007, respectively), no significant differences were observed in the expression of these two genes between swimming training group and control cancer group (p=0.056, p=0.18, respectively). Finally, our result indicated that training + royal jelly group had the greatest decrease in expression of JNK and AP-1genes compared to the control cancer group (both p=0.001).
Conclusion: According to the study results, it seems that swimming training along with the consumption of royal jelly has more inhibitory and protective effects on the expression of JNK and AP-1 genes in lung tissue compared to either of these interventions in mice with lung cancer. To generalize the results to human, further studies on human subjects are warranted.

کلیدواژه‌ها [English]

  • Swimming training
  • Royal jelly
  • JNK
  • AP-1
  • Lung cancer

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  • تاریخ دریافت: 21 خرداد 1404
  • تاریخ بازنگری: 26 مرداد 1404
  • تاریخ پذیرش: 05 شهریور 1404
  • تاریخ اولین انتشار: 05 شهریور 1404
  • تاریخ انتشار: 01 دی 1404

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