تأثیر شش هفته تمرین تناوبی شدید شنا و مکمل رزوراترول بر میزان SIRT3در بافت بطن چپ قلب موش‌های صحرایی پیر

نوع مقاله : علمی - پژوهشی

نویسندگان

1 دانشکدة تربیت بدنی و علوم ورزشی، پردیس دانشگاه تهران، کیش، ایران

2 دانشکدة تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران

چکیده

زمینه و هدف: شناسایی و تجزیه‌وتحلیل اجزایی که در به تأخیر انداختن پیری و افزایش طول عمر نقش دارند، بسیار ضروری است. سیرتوئین‌ها از تنظیم‌کننده‌های اصلی بقای سلولی و طول عمر هستند. هدف پژوهش حاضر بررسی تأثیر شش هفته فعالیت ورزشی HIIT شنا و مکمل رزوراترول بر مقدار SIRT3 در بافت بطن چپ قلب موش‌های صحرایی پیر بود.
مواد و روش‌ها: در این پژوهش، 30 سر موش صحرایی 20 ماهه (پیر) نر نژاد ویستار تصادفی به پنج گروه (کنترل (C)، گروه تمرین (EX)، گروه تمرین+مکمل (EXR)، گروه مکمل (R) و گروه حلال (M)) تقسیم شدند. گروه EX، تمرین HIIT  شنا را به مدت شش هفته انجام دادند. گروه C، تمرین نمی‌کردند. گروه R، فقط مکمل رزوراترول دریافت کردند. گروه EXR، تمرین HIIT  شنا را همراه با دریافت رزوراترول انجام دادند. گروه M فقط حلال دریافت کردند. SIRT3 با استفاده از روش آزمایشگاهی وسترن بلات سنجیده شد. داده‌های حاصله توسط آزمون آماری تحلیل واریانس یکطرفه و آزمون تعقیبی توکی تجزیه‌وتحلیل شدند.
نتایج: پس از شش هفته، تفاوت معناداری بین میانگین میزان پروتئین SIRT3 در پنج گروه مشاهده شد. بنابراین، آزمون تعقیبی توکی نشان داد که مقدار SIRT3 در گروه EX و EXR در مقایسه با گروه C، R و M، به‌طور معناداری بیشتر بود (001/0P=)، اما این تفاوت بین دو گروه EX و EXR معنادار نبود (05/0P>). همچنین، مقدار SIRT3 در گروه R به‌طور معناداری در مقایسه با گروه C و M بیشتر بود.
نتیجه‌گیری: افزایش SIRT3، نشان‌دهندة دخیل بودن این پروتئین در مسیرهای سوخت‌وسازی، دفاع ضداکسایشی و بهبود وضعیت سلول است. همچنین می‌توان از تمرین تناوبی شدید همراه با مصرف مکمل رزوراترول برای تعدیل فشار اکسایشی و تأخیر روند پیری و سالمندی بهره برد.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of six weeks High Intensity Interval Swimming Training and Resveratrol supplementation on the level of SIRT3 in left ventricular heart of aged rats

نویسندگان [English]

  • Ali Reza Rezaei 1
  • Abbas Ali Gaeini 2
  • Sirous Choobineh 2
  • Reza Nuri 1
1 Faculty of Physical Education and Sport Sciences, University of Tehran Kish International Campus, Kish, Iran
2 Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
چکیده [English]

Background and Purpose: Identification and analysis of compounds that playing a role in delay aging and increase longevity is essential. Sirtuins are one of the main regulators of cell survival and longevity. The goal of this research was to investigate the effect of six weeks HIIT swimming exercise and Resveratrol supplementation on the level of SIRT3 in left ventricular heart of aged rats.
Materials and Methods: In this research, 30 wistar rats were randomly divided into five groups (control (C), solvent (M), supplement (R), HIIT exercise (EX), HIIT exercise and supplement (EXR)). The EX group performed HIIT swimming for six weeks. C group did not practice. R group received only resveratrol supplementation. EXR group performed HIIT swimming exercises with resveratrol and M group received only solvent. The SIRT3 protein was obtained by Western blotting. Data were analyzed using One-way ANOVA analysis of variance and Tukey's post hoc test.
Results: After six weeks, there was a significant difference between the mean SIRT3 protein levels in the five groups. Therefore, Tukey post hoc test showed that SIRT3 levels were significantly increased in EX and EXR groups compared to C, R and M groups (P = 0/001). However, this difference between EX and EXR groups was not significant (P > 0.05). Moreover, the amount of SIRT3 in group R was significantly higher compared to groups C and M.
Conclusion: Increasing the amount of SIRT3 indicates the involvement of this protein in metabolic pathways, antioxidant defense and improves cell condition. High intensity interval training with resveratrol supplement consumption can also be used to moderate oxidative stress and delay the aging process.

کلیدواژه‌ها [English]

  • Life span
  • Exercise Training
  • Antioxidant Supplements

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سابقه مقاله

  • تاریخ دریافت: 24 آبان 1400
  • تاریخ بازنگری: 08 دی 1400
  • تاریخ پذیرش: 11 دی 1400
  • تاریخ اولین انتشار: 31 خرداد 1401
  • تاریخ انتشار: 01 تیر 1401

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