تأثیر مداخلة همزمان تمرین هوازی تناوبی و دریافت عصارة کرم آرد بر بیان ژن‌های CHOP و BIP و نشانگرهای فشار اکسایشی MDA و SOD بافت کبد موش‌های صحرایی مبتلا به کبد چرب غیرالکلی

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه فیزیولوژی ورزش، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران

چکیده

زمینه و هدف: تنظیم اتوفاژی، تنش شبکۀ اندوپلاسمی (ER)، فعالیت لیزوزومی و ارتباط آن‌ها با فشار اکسایشی به‌عنوان اهداف زیربنایی یاخته‌ای در درمان کبد چرب غیرالکلی (NAFLD) مطرح شده‌اند. امروزه بررسی تغییرات حاصل از مداخلات درمانی NAFLD به‌ویژه ورزش و اصلاح رژیم غذایی بر برخی نشانگرهای کبدی در اتوفاژی، تنش یاخته‌ای و سوخت‌وساز چربی ضروری به‌نظر می‌رسد. از این‌رو، این پژوهش با هدف بررسی تأثیر مداخلۀ همزمان تمرین هوازی تناوبی و دریافت عصارۀ کرم آرد بر بیان ژن‌های پروتئین ایمونوگلوبولین پیوندی (BIP)، پروتئین همولوگ C/EBP (CHOP)، مالون دی آلدئید (MDA) و سوپراکساید دیسموتاز (SOD) بافت کبد موش‌های صحرایی مبتلا به کبد چرب غیرالکلی انجام گرفت.
مواد و روش‌ها: این پژوهش تجربی و کاربردی، روی 25 سر موش صحرایی نر ویستار انجام شد که به پنج گروه مساوی شامل 1. سالم، 2. بیمار، 3. بیمار+ مکمل، 4. بیمار+ ورزش، 5. بیمار+ مکمل+ ورزش تقسیم شدند. به‌منظور القای NAFLD، نمونه‌ها به مدت 12 هفته با رژیم غذایی پرچرب، کلسترول و اسید کولیک تغذیه شدند و گروه سالم، رژیم غذایی استاندارد جوندگان را دریافت کردند. پس از اطمینان از القای بیماری، تمرین هوازی تناوبی با شدت متوسط، هشت هفته، پنج جلسه در هفته دویدن روی نوار گردان (در مجموع 30 دقیقه) همراه با اضافه بار تدریجی انجام شد. گاواژ مکمل عصارۀ کرم آرد، با دوز 20 میلی‌گرم/کیلوگرم وزن بدن مشابه با روزهای ورزش صورت گرفت. اندازه‌گیری بیان کبدی ژن‌های BIP و CHOP به روش Real-Time PCR و غلظت‌های کبدی MDA و SOD به روش وسترن بلات صورت گرفت. داده‌های حاصل از طریق آزمون‌های آماری t-مستقل و تحلیل واریانس دوعاملی در سطح معناداری 05/0P≤ بررسی شدند.
نتایج: در موش‌های صحرایی با الگوی NAFLD، افزایش سطوح کبدی BIP، CHOP و MDA و کاهش سطح SOD دیده شد. تمرین و دریافت عصارۀ کرم آرد، هر کدام به‌تنهایی و همچنین در تعامل با یکدیگر به کاهش معنادار سطوح BIP، CHOP و MDA و افزایش معنادار SOD در موش‌های مبتلا به NAFLD منجر شدند (01/0P≤)؛ اندازۀ اثر تمرین نسبت به مکمل تنها یا تعامل تمرین و مکمل، فقط در افزایش SOD بالاتر بود.
 نتیجه‌گیری: بر پایۀ یافته‌های پژوهش گمان می‌رود مداخلات همزمان تمرین تناوبی هوازی به‌همراه دریافت عصارۀ کرم آرد از طریق کاهش انباشت چربی کبدی، تنش ER و فشار اکسایشی و نقش آن‌ها در تنظیم اتوفاژی در درمان NAFLD مؤثر باشد. نیاز به پژوهش‌های بیشتر به‌منظور روشن شدن سازوکار اثر این مداخلات پژوهشی بر ژن‌های BIP و CHOP و نقش میانجیگری آن‌ها در تنظیم اتوفاژی و تنش یاخته‌ای احساس می‌شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of simultaneous intervention of interval aerobic training and mealworm extract on the expression of CHOP and BIP genes and oxidative stress markers of MDA and SOD in liver tissue of rats with non-alcoholic fatty liver disease

نویسندگان [English]

  • Zohreh Bagherpour
  • Alireza Rahimi
  • Fariba Aghaei
  • Foad Feizolahi
Department of exercise physiology, Karaj branch, Islamic Azad University, Karaj, Iran
چکیده [English]

Background and Purpose: Regulation of autophagy, endoplasmic reticulum (ER) stress, lysosomal activity and their relationship with oxidative stress have been proposed as underlying cellular targets in the treatment of non-alcoholic fatty liver disease (NAFLD). Nowadays, it seems necessary to investigate the changes resulting from NAFLD therapeutic interventions, especially exercise and dietary modification, on some hepatic markers in autophagy, cellular stress, and lipid metabolism. Therefore, the aim of this study was to investigate the simultaneous effects of interval aerobic training and mealworm extract on the expression of genes of bound immunoglobulin protein (BIP), C/EBP homolog protein (CHOP), malondialdehyde (MDA), and superoxide dismutase (SOD) in liver tissue of rats with nonalcoholic fatty liver.
Materials and Methods: This experimental and applied study was conducted on 25 male Wistar rats, which were divided into five equal groups: 1) healthy, 2) diseased, 3) diseased + supplement, 4) diseased + exercise, 5) diseased + supplement + exercise. To induce NAFLD, the samples were fed a high-fat, cholesterol, and cholic acid diet for 12 weeks, and the healthy group received a standard rodent diet. After ensuring the induction of the disease, a moderate-intensity interval aerobic training protocol was performed, 8 weeks/5 sessions per week of treadmill running for a total of 30 minutes with gradual overload. The Mealworm extract supplement gavage was performed at a dose of 20 mg/kg body weight similar to the exercise days. Hepatic expression of BIP and CHOP genes was measured by Real-Time PCR and hepatic MDA and SOD concentrations were measured by Western blotting. Data were analyzed using independent t-tests and two-way analysis of variance.
Results: Hepatic levels of BIP, CHOP, and MDA were increased and SOD level was significantly decreased in NAFLD group. Exercise and mealworm extract separately and in interaction with each other, resulted in a significant decrease in BIP, CHOP, and MDA levels and a significant increase in SOD in NAFLD rats (p<0.01). The effect size of exercise was higher than that of supplementation alone or the interaction of exercise and supplementation, only for SOD.
Conclusion: Employing aerobic interval training along with mealworm extract intake simultanuously may be effective in the treatment of NAFLD, possibly through reducing hepatic fat accumulation, ER stress, and oxidative stress, and their role in regulating autophagy. Further research is needed to clarify the mechanism of the effect of these interventions on BIP and CHOP genes and their mediating role in regulating autophagy and cellular stress.
 
 

کلیدواژه‌ها [English]

  • Exercise
  • Mealworm
  • C/EBP Homologous protein
  • Superoxide dismutase

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  • تاریخ دریافت: 28 فروردین 1404
  • تاریخ بازنگری: 02 خرداد 1404
  • تاریخ پذیرش: 10 تیر 1404
  • تاریخ اولین انتشار: 10 تیر 1404
  • تاریخ انتشار: 01 فروردین 1405

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