تاثیر تمرین شنا و قرارگیری در معرض نیتروز آمین کتون مشتق از تنباکو ) NNK ( بر پاسخ ایمونوهیستوشیمیایی ماکروفاژهای مرتبط با تومور در موش های صحرایی

نوع مقاله : علمی - پژوهشی

نویسندگان

1 گروه تربیت بدنی و علوم ورزشی، دانشکده علوم تربیتی و روان شناسی، دانشگاه پیام نور، تهران، ایران

2 گروه فیزیولوژی ورزش، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه مازندران، بابلسر، ایران

3 گروه پاتولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی بابل، بابل، ایران

چکیده

هدف: نیتروز آمین کتون مشتق از تنباکو ) NNK (، یکی از قویترین کارسینوژنهای تنباکو است که با تغییر در آزادسازی
ماکروفاژهای مرتبط با تومور ) TAM ( در القای سرطان ریه مؤثر است. هدف از این مقاله، تأثیر تمرین شنا و قرارگیری
در معرض نیتروز آمین کتون مشتق از تنباکو ) NNK ( بر پاسخ ایمونوهیستوشیمیایی ماکروفاژهای مرتبط با تومور در
موشهای صحرایی است.
روشها: بدین منظور 46 سر موش صحرایی نر بالغ ویستار به صورت تصادفی به پنج گروه تمرین، تمرین+ ،NNK، NNK
حلال و کنترل دسته بندی شدند. گروه های تحت القای NNK به صورت زیر جلدی 1 بار در هفته و به میزان 5/ 12 میلی
گرم بر کیلوگرم وزن بدن در 12 هفته NNK دریافت کردند. گروه های تمرین نیز به مدت 12 هفته به شنا زیر بیشینه
پرداختند. بیان ماکروفاژهای مرتبط با تومور در بافت ریه با روش ایمونوهیستوشیمی اندازه گیری شد. با استفاده از تحلیل
واریانس یک-طرفه و آزمون پیگیری توکی در سطح معنی داری 05 / P≥0 استفاده شد.
نتایج : نتایج نشان داد که بیان ایمونوهیستوشیمیایی TAM در گروه تمرین نسبت به گروه P= 0/001( NNK (، به
طور معنیداری کمتر بود ) 92 %(. بیان ایمونوهیستوشیمیایی TAM نیز در گروه تمرین+ NNK نسبت به گروه NNK
P=0/001( ( به طور معنیداری کمتر بود ) 92 %(. علاوه بر این، بیان ایمونوهیستوشیمیایی TAM در گروه NNK ، نسبت
به گروه حلال ) 001 / P= 0 ( به طور معنیداری بیشتر بود ) 766 %(.
نتیجه گیری: به طور کلی میتوان اذعان داشت که تمرین ورزشی منظم هوازی زیر بیشینه، در مهار آثار التهابی ریه ناشی
از القای NNK با کاهش میزان فعالیت ماکروفاژهای مرتبط با تومور نقش مؤثری دارد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of swimming training and exposure to nicotine derived nitrosamine ketone (NNK) on the immunohistochemical response of tumor-associated macrophages in wistar rats

نویسندگان [English]

  • Ali Barzegari 1
  • Shadmehr Mirdar 2
  • Mohammad Ranaei 3
1 Department of physical education, Faculty of Educational Sciences and Psychology, Payame Noor University, Tehran, Iran
2 Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Mazandaran, Mazandaran, Iran
3 Department of Pathology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
چکیده [English]

Purpose: The nicotine derived nitrosamine ketone (NNK) is one of the strongest carcinogens in tobacco that involved in induction of lung cancer by changing the stimulation of tumor associated macrophages. The aim of this study was to effect of swimming training and exposure to nicotine derived nitrosamine ketone (NNK) on the immunohistochemical response of tumor-associated macrophages in wistar rats.
Methods: For this purpose, 46 Wistar rats were randomly divided into five groups consist of training, training + NNK, NNK, saline and control. NNK-induced groups received NNK subcutaneously one day per week at a rate of 12/5 mg per kg body weight and the training groups performed submaximal swimming training for 12 weeks. Levels of CD68-Tumor associated macrophages in homogenized lung tissue were measured by immunohistochemistry. To analyze the data; ANOVA and tukey’s test were used at a significance level of p≤0.05.
Results: The results showed that immunohistochemical expression of TAM in the training group was significantly lower than the NNK group (P = 0.001) (92%). Immunohistochemical expression of TAM was also significantly lower in the NNK + training group than in the NNK group (P = 0.001) (92%). In addition, the immunohistochemical expression of TAM in the NNK group was significantly higher than the solvent group (P = 0.001) (766%).
Conclusion: Generally, it could be confirmed that regular submaximal swimming training plays an important role in inhibition of the effects of lung inflammation induced by NNK via decreased of Tumor associated macrophages activity.

کلیدواژه‌ها [English]

  • Nicotine derived nitrosamine ketone
  • Submaximal swimming training
  • Tumor associated macrophages
  • Immunohistochemistry
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