اثر12 هفته تمرین هوازی بر غلظت گرلین و هورمون‌های انسولین، رشد و کورتیزول پلاسمایی و رابطه بین آنها در موش‌های نر

نوع مقاله : علمی - پژوهشی

نویسندگان

1 داﻧﺸﮕﺎه آزاد اﺳﻼﻣﯽ

2 داﻧﺸﮕﺎه ﺷﻬﯿﺪ ﺑﻬﺸﺘﯽ

چکیده

هدف: هدف این پژوهش تعیین تغییرات گرلین پلاسما به دنبال12 هفته تمرین هوازی با شدت متوسط در موش‌های نر صحرایی و همچنین بررسی ارتباط بین تغییرات گرلین تام پلاسما با تغییرات هورمون‌های انسولین، رشد وکورتیزول بود. روش‌ها: برای انجام این تحقیق تعداد 20 سر موش به دو گروه کنترل(9=n) و تمرین(11=n) تقسیم شدند. موش‌های گروه تمرین به مدت 12 هفته، یک برنامه تمرین استقامتی با شدت متوسط دویدن روی نوارگردان بدون شیب (به مدت 60 دقیقه با سرعتm/min 25 معادل Vo2max%65 برای 5 جلسه در هفته) را اجرا نمودند. بعد از اتمام دوره تمرین و به فاصله 48 ساعت پس از آخرین جلسه تمرین نمونه‌های خون جمع آوری شد و برای اندازه‌گیری گرلین، گلوکز، انسولین، هورمون رشد و کورتیزول تحلیل شدند. داده-های دو گروه با استفاده از t مستقل مقایسه شدند. نتایج: سطوح گرلین پلاسما در گروه کنترل 85/25±68/86 پیکوگرم بر میلی‌لیتر بود.این مقدار درگروه تمرین به مقدار41/5±72/59 پیکوگرم بر میلی‌لیتر کاهش داشت و از نظر آماری معنی‌دار بود (0001/0>P و 9/6 = t). با این حال، 12 هفته تمرین استقامتی تاثیر معنی‌داری بر وزن موش‌ها (80/0=p ،258/ 0=t) سطوح گلوکز(262/0=p ،15/1=t)، انسولین(1/0=p , 73/1=t) و هورمون رشد(97/0=p ,75/1- =t) وکورتیزول (084/0=p,82/1- =t) نداشت .نتایج ضریب همبستگی نیز ارتباط معنی داری را بین گرلین وگلوکز، انسولین، هورمون رشد وکورتیزول نشان نداد (05/0p>). بحث و نتیجه‌گیری : نتیجه پژوهش حاضر نشان داد که 12 هفته دویدن بر روی نوارگردان با شدت متوسط منجر به کاهش معنی‌دار سطوح پلاسمایی گرلین در موش‌های نر صحرایی شد و تاثیر معنی‌داری بر سطوح متغیرهای دیگر و رابطه بین آنها نشان نداد. می‌توان نتیجه گرفت تغییرات گرلین می‌تواند تابعی از عوامل دیگر مانند مدت و شدت تمرین و ناشتایی همراه با کاهش معنی‌دار وزن باشد و نیز تغییرات گرلین و هورمون رشد را می‌توان مستقل از هم دانست.

کلیدواژه‌ها

عنوان مقاله [English]

The effects of 12 weeks of aerobic exercise on plasma Ghrelin, Insulin, GH, Cortisol Hormones concentration in male rats.

چکیده [English]

 
Abjectivs: The purpose of this study was to investigate the effects of 12 weeks of treadmill running with modrate intensities on plasma Ghrelin and Glucose, Insulin, GH, Cottisol Hormon concentrations and relationship between this variable in male rat. Methods: Towenty Wistar male rats were used and randomly divided into two groups. control (n=9) and training group (n=11). The training group were given exercise on a motor-driven treadmil! (25m٫min. 60min. and 5 days٫weeks) for 12 weeks. Rats were sacrificed 48h after last training session. Plasma samples were collected, Ghrelin were meseared by ELISA and data were analyzed by T test, significance level was accepted at P<0.05 Results: The resalts indicate that plasma Ghrelin concentration in control(86.68+25.85pg٫1) and trained (59.72±5.41pg٫1) group that significantly decreased (t=6.9,P=0.0001) .Changes in weitgh (t=0.258.p=0.8) plasma glucose z(t=1.15.p=0.262) insulin (t=1.73.p=0.1). GH(1-1.75.pand cortisol (t=1.82.p=0.084) levels were not significant. Pearson correlation between the grelin and glucose, insulin, GH and cortisol concentrations assessed were not significant (P>0.05) Conclusion: it is concluded that long term treadmill exercise was effective on plasma ghrelin that this changes so changes of Insulin.GH and cortisol hormone can be related to time and intensity of training and time of fasting and rate of decrease of weight in exercise training.Also plasma ghrelin and GH concentration can be independent.
 

کلیدواژه‌ها [English]

  • Aerobic Exercise training
  • Ghrelin
  • Rat
  • Glucose
  • Insulin
  • GH hormone
  • Cortisol

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سابقه مقاله

  • تاریخ دریافت: 27 اردیبهشت 1394
  • تاریخ بازنگری: 29 خرداد 1403
  • تاریخ پذیرش: 11 دی 1399
  • تاریخ اولین انتشار: 11 دی 1399
  • تاریخ انتشار: 01 اردیبهشت 1391

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