Journal of Sport and Exercise Physiology

Journal of Sport and Exercise Physiology

Comparison the Influence of Various Intensities of Aerobic Training on the Expression of RBL-1 and RB1 Genes in the Subcutaneous Adipose Tissue of Male Wistar Rats

Document Type : original article

Authors
Maryam Shavandi 1
Saeid Naghibi 2
Mohammad Shariatzadeh Joneydi 3
Maryam Vatandoust 2
Ali Zare 4
1 Department of Physical Education, Payame Noor University, Alborz, Iran
2 Department of Physical Education and Sport Sciences, Payame Noor University (PNU), Tehran, Iran
3 Institute of Physical Education and Sport Sciences, Tehran, Iran
4 Department of Physical Education, Islamic Azad University of Karaj, Alborz, Iran
10.52547/joeppa.15.3.35
Abstract
Background and Purpose: One of the most important methods to cope with obesity metabolic disorder is to do exercise activities that are both effective as a prevention and treatment. Since the expression of adipogenic genes such as Retinoblastoma-1 (RB1) and Retinoblastoma like-1 (RBL-1) proteins are effective in adipogenesis, the aim of this study was to investigate the influence of various intensities of aerobic training on the expression of RB1 and RBL-1 genes in the subcutaneous adipose tissue of male Wistar rats.
Materials and Methods: In this experimental study, 32 male Wistar rats (eight weeks old and weight: 237 ± 33 grams) were randomly divided into four equal groups: control, high intensity training (HIT), Moderate-intensity training (MIT) and high-intensity interval training (HIIT). The treadmill training protocols consisted of eight weeks, so that the HIT training consisted of running at a speed of 20 meters per minute or with an intensity of 65% of maximum oxygen consumption (VO2max), with a gradual increasing slope for 30 minutes. MIT training consisted of running at 65% VO2max for 37 minutes and HIIT training consisting of four bouts of high-intensity intervals with four minutes running at 90 to 100% VO2max and four bouts of low-intensity intervals with three minutes running at 50 to 60% VO2max (28 minutes in total). 24 hours after the last training session, the animals were sacrificed and their subcutaneous fat tissue was removed and gene expression was measured by RT-PCR. The obtained data were analyzed due to lack of natural distribution using Kruskal-wallis test and Bonferoni post hoc test through SPSS statistical software version 24 And significance level was considered P < 0.05.
Results: The results of the present study showed that the expression of RB1 gene was significantly reduced only in the MIT group compared to the control group (P = 0.027). Also, RBL-1 gene expression was significantly lower only in the HIT group than in the control group (P = 0.028).
Conclusion: Since in this study only MIT and HIT aerobic exercises with 65% VO2max intensity could reduce the expression of RB1 and RBL-1 genes, the use of this type of exercise to improve metabolic disorders and inhibit adipogenesis is recommended.
Keywords
Aerobic Training
Subcutaneous Fat
Retinoblastoma Protein
Obesity
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  • Receive Date 02 December 2021
  • Revise Date 12 February 2022
  • Accept Date 12 February 2022
  • First Publish Date 21 June 2022
  • Publish Date 22 June 2022