The effect of intensity exercises training on Catalase Enzyme Gene Expression in soccer players

doi:10.48308/joeppa.2015.98723

The effect of intensity exercises training on Catalase Enzyme Gene Expression in soccer players

Document Type : original article

10.48308/joeppa.2015.98723

Abstract

Purpose: Studies have shown that intense exercise training associated conditions with increases levels of free
radicals and oxidative stress thus the aim of this study was to investigate effect of intense exercises on
Catalase Enzyme Gene Expression in soccer players. Method: Nine male soccer players amateur (16-18age)
for Among the players who participated in the study volunteers were randomly selected and participated in
this study. blood samples were taken in three stages, before GXT exercise test (graduate exercise test),
immediately and 3 hours after exercise to determine mRNA of catalase. Results : Findings showed no
significant increase in the mRNA catalase of lymphocyte in young soccer players immediate (p=0.17)
and 3 hour after incremental exercise (p=0.08). Conclusion: According to the results of this study, it was
determined that intense exercise training causes no significant increase in the mRNA catalase of lymphocyte
in young soccer. This may be due to the adaptation process of catalase mRNA transcription lymphocytes in
football players.

Keywords

References

ViÃ±a J, Borras C, Abdelaziz KM, Garcia-Valles R, Gomez-Cabrera MC.(2013).The Free Radical Theory of Aging Revisited: The Cell Signaling Disruption Theory of Aging. Antioxidants & redox signaling. 19(8):779-87.
Finkel T, Holbrook NJ.(2000). Oxidants, oxidative stress and the biology of ageing. Nature. 408(6809):239-47.
Guemouri L, Artur Y, Herbeth B, Jeandel C, Cuny G, Siest G.(1991). Biological variability of superoxide dismutase, glutathione peroxidase, and catalase in blood. Clinical Chemistry. 37(11):1932-7.
Halliwell B, Gutteridge JM.(1999). Free radicals in biology and medicine: Oxford university press Oxford.
Stryer L.(1995). Biochemistry. WH Freeman, New York. 6. Ji LL.(1999). Antioxidants and oxidative stress in exercise. Experimental Biology and medicine. 222(3):283-92.
Murray K, Rodwell V, Bender D, Botham KM, Weil PA, Kennelly PJ.(2009). Harper's Illustrated Biochemistry. 28: New York: McGraw-Hill.
Kirkman HN, Gaetani GF.(2007). Mammalian catalase: a venerable enzyme with new mysteries. Trends Biochem Sci. 32(1):44-50.
Alpay Z, Saed GM, Diamond MP.(2006). Female infertility and free radicals: potential role in adhesions and endometriosis. Journal of the Society for Gynecologic Investigation. 13(6):390-8.
Pepe H, Balci ÅS, Revan S, Akalin PP, KurtoÄlu F.(2009). Comparison of oxidative stress and antioxidant capacity before and after running exercises in both sexes. Gender medicine. 6(4):587-95.
Metin G, Gumustas M, Uslu E, Belce A, Kayserilioglu A.(2003). Effect of regular training on plasma thiols, malondialdehyde and carnitine concentrations in young soccer players. Chinese Journal of Physiology. 46(1):35-9.
Yano T, Yunoki T, Matsuura R, Arimitsu T, Kimura T.(2007). Excessive oxygen uptake during exercise and recovery in heavy exercise. Physiological Research. 56:721-5.
Aguilo A, Tauler P, Fuentespina E, Tur JA, Cordova A, Pons A.(2005). Antioxidant response to oxidative stress induced by exhaustive exercise. Physiology & behavior. 84(1):1-7.
Kiyici F, Kishali N.(2012). Acute effect of intense exercises on serum superoxide dismutase, catalase and malondialdehyde levels in soccer players. Journal of sports medicine and physical fitness. 52(1):107-11.
Heyward VH.(2006). Advanced fitness assessment and exercise prescription: Human Kinetics Publishers.
Cohen G, Dembiec D, Marcus J.(1970). Measurement of catalase activity in tissue extracts. Analytical biochemistry. 34(1):30-8.
Tauler P, Aguilo A, Gimeno I, Guix P, Tur JA, Pons A. (2004).Different effects of exercise tests on the antioxidant enzyme activities in lymphocytes and neutrophils. The Journal of Nutritional Biochemistry. 15(8):479-84.
Eskes T, Haanen C.(2007). Why do women live longer than men? European Journal of Obstetrics & Gynecology and Reproductive Biology. 133(2):126-133.
Radak Z.(2000). Free radicals in exercise and aging. Human Kinetics Europe.
Tauler P, Sureda A, Cases N, Aguilo A, Rodriguez-Marroyo JA, Villa G, et al.(2006). Increased lymphocyte antioxidant defences in response to exhaustive exercise do not prevent oxidative damage. The Journal of nutritional biochemistry. 17(10):665-71.
Cases N, Sureda A, Maestre I, Tauler P, Aguilo A, Cordova A, et al.(2006). Response of antioxidant defences to oxidative stress induced by prolonged exercise: antioxidant enzyme gene expression in lymphocytes. European journal of applied physiology. 98(3):263-9.