The effect of endurance training and iron supplementation on some cellular respiration factors in rats

Samavati Shari, Mohammad Ali; Ravasi, Aliasghar; Kordi, Mohammad Reza; Minaie, Bagher; Hojjatollah, Siavoshy

doi:10.48308/joeppa.2018.98909

The effect of endurance training and iron supplementation on some cellular respiration factors in rats

Document Type : original article

Authors

¹ Faculty of Sport Sciences, University of Bu-Ali Sina, Hamedan, Iran

² Faculty of Sport Sciences, University of Tehran, Tehran, Iran

³ 3 Medical histologist traditional medicine tehran university medical science,Tehran, Iran

⁴ Department of Exercise Physiology, Sports Medicine Research Center, Sport Sciences Research Institute, Tehran, Iran

10.48308/joeppa.2018.98909

Abstract

Purpose: Iron plays an important role in oxygen transfer, mitochondrial respiratory chain enzymes, and
oxidative phosphorylation; therefore, the reduction of iron can have a negative effect on the oxidative
performance of athletes. The aim of study was investigate the effect of endurance exercise programs and
iron supplementation on some cellular respiration factors in rats.
Methods: Forty male wistar-rats were divided into four groups. experimental Group I, do endurance exercise
programs on treadmill in during 12 weeks (intensity 32 m.min-1, 60 minutes in every session, and 5 sessions
in a week) (T). Experimental group II do same exercise program, but intake daily 800 micrograms iron
supplementary (Ferrous-Sulphate) (Ti). The control group C was without exercise (sedentary) (S), and
experimental group III, did not do any exercise, but received an 800 microgram daily iron supplement (Si).
After 12 weeks, their blood and muscle tissue samples were analyses. Data were analyzed using one wayANOVA and Kruskal-Wallis Test (P<0.05).
Results: Blood ferritin concentration only in the experimental group I and absolute weight of soleus muscle
and cytochrome C oxidase only in the experimental group II than other groups was statistically significant
(p<0.05). Also, muscle hypertrophy, capillary density, and mitochondrial density in the experimental groups I
and II compared with the control group and experimental group III were significantly different (p<0.05); But,
this differences between both experimental groups I and II were not significant. Despite, body mass, relative
weight of soleus muscle, and maximum oxygen consumption in the Experimental groups compared with the
control group were significantly different and these differences between the experimental groups also were
significant (p<0.05).
Conclusions: It seems that iron supplementations combined with exercises maybe improve some markers of
cellular respiration, hypertrophy, and aerobic capacity; Despite, these improvements probably do not occur
in detraining.

Keywords

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