The effect of 8 weeks IGF-1 injection and resistance training on Cox-2 and p53 expression in colorectal of male Wistar rats

Document Type : original article

Authors

1 PhD student in Sport Physiology, Faculty of Physical Education and Sport Sciences, Ferdowsi University of Mashhad, Mashhad, Iran

2 Department of Sport Physiology, Faculty of Physical Education and Sport Sciences, Ferdowsi University of Mashhad, Mashhad, Iran

Abstract

Abstract
Purpose: The aim of this study was to determine the effect of 8 weeks IGF-1 injection and resistance training on Cox-2 and p53 expression in colorectal of male Wistar rats. Methods: A total of 28 male Wistar rats were randomly divided into 4 groups; saline injected control (Control), resistance training+saline injected (Training), resistance training+IGF-1 injected (Combined) and IGF-1 injected (Injection). Resistance training protocol consisted of climbing (three days/week, 5 reps/3 sets) a ladder carrying a load suspended from the tail for 8 weeks. IGF-1 and saline (1.5 µg/kg/day) were injected before and after exercise sessions. Three days after the last bout of exercise, colorectal tissues were collected and gene expression levels were analyzed using real-time PCR. Results: In Training, Combined and Injection groups, p53 expression in the colorectal decreased compared with Control (p

Keywords


  1. McGuire S. World cancer report 2014. Geneva, Switzerland: World Health Organization, international agency for research on cancer, WHO Press, 2015. Advances in Nutrition: An International Review Journal. 2016;7(2):418-9.
  2. Wang L, Shen X, Wang Z, Xiao X, Wei P, Wang Q, et al. A molecular signature for the prediction of recurrence in colorectal cancer. Molecular cancer. 2015;14(1):22.
  3. Abdifard E, Ghaderi S, Hosseini S, Heidari M. Incidence trends of colorectal cancer in the West of Iran during 2000-2005. Asian Pac J Cancer Prev. 2013;14(3):1807-11.
  4. Siegel R, Ma J, Zou Z, Jemal A. Cancer statistics, 2014. CA: a cancer journal for clinicians. 2014;64(1):9-29.
  5. Marmot M, Atinmo T, Byers T, Chen J, Hirohata T, Jackson A, et al. Food, nutrition, physical activity, and the prevention of cancer: a global perspective. 2007.
  6. Shanmugalingam T, Bosco C, Ridley AJ, Van Hemelrijck M. Is there a role for IGF‐1 in the development of second primary cancers? Cancer medicine. 2016;5(11):3353-67.
  7. Li X-L, Zhou J, Chen Z-R, Chng W-J. P53 mutations in colorectal cancer-molecular pathogenesis and pharmacological reactivation. World journal of gastroenterology: WJG. 2015;21(1):84.
  8. Soslow RA, Dannenberg AJ, Rush D, Woerner B, Khan KN, Masferrer J, et al. COX‐2 is expressed in human pulmonary, colonic, and mammary tumors. Cancer. 2000;89(12):2637-45.
  9. Iwama T. NSAIDs and colorectal cancer prevention. Journal of gastroenterology. 2009;44:72-6.
  10. Meric J-B, Rottey S, Olaussen K, Soria J-C, Khayat D, Rixe O, et al. Cyclooxygenase-2 as a target for anticancer drug development. Critical reviews in oncology/hematology. 2006;59(1):51-64.
  11. Chen S, Cao W, Yue P, Hao C, Khuri FR, Sun S-Y. Celecoxib promotes c-FLIP degradation through Akt-independent inhibition of GSK3. Cancer research. 2011;71(19):6270-81.
  12. Wei D, Wang L, He Y, Xiong HQ, Abbruzzese JL, Xie K. Celecoxib inhibits vascular endothelial growth factor expression in and reduces angiogenesis and metastasis of human pancreatic cancer via suppression of Sp1 transcription factor activity. Cancer research. 2004;64(6):2030-8.
  13. Neeman E, Zmora O, Ben-Eliyahu S. A new approach to reducing postsurgical cancer recurrence: perioperative targeting of catecholamines and prostaglandins. Clinical Cancer Research. 2012;18(18):4895-902.
  14. Höpker K, Hagmann H, Khurshid S, Chen S, Schermer B, Benzing T, et al. Putting the brakes on p53-driven apoptosis. Cell Cycle. 2012;11(22):4122-8.
  15. Iacopetta B. TP53 mutation in colorectal cancer. Human mutation. 2003;21(3):271-6.
  16. Kaaks R, Lukanova A. Energy balance and cancer: the role of insulin and insulin-like growth factor-I. Proceedings of the Nutrition Society. 2001;60(1):91-106.
  17. Giovannucci E. Insulin, insulin-like growth factors and colon cancer: a review of the evidence. The Journal of nutrition. 2001;131(11):3109S-20S.
  18. Herndon DN, Ramzy PI, DebRoy MA, Zheng M, Ferrando AA, Chinkes DL, et al. Muscle protein catabolism after severe burn: effects of IGF-1/IGFBP-3 treatment. Annals of surgery. 1999;229(5):713.
  19. Holt R, Sönksen P. Growth hormone, IGF‐I and insulin and their abuse in sport. British journal of pharmacology. 2008;154(3):542-56.
  20. Sandhu MS, Dunger DB, Giovannucci EL. Insulin, insulin-like growth factor-I (IGF-I), IGF binding proteins, their biologic interactions, and colorectal cancer. Journal of the National Cancer Institute. 2002;94(13):972-80.
  21. Levine AJ, Feng Z, Mak TW, You H, Jin S. Coordination and communication between the p53 and IGF-1–AKT–TOR signal transduction pathways. Genes & Development. 2006;20(3):267-75.
  22. Devin JL, Bolam KA, Jenkins DG, Skinner TL. The influence of exercise on the insulin-like growth factor axis in oncology: physiological basis, current, and future perspectives. Cancer Epidemiology and Prevention Biomarkers. 2015;25(2):239-49.
  23. Yamauchi M, Lochhead P, Imamura Y, Kuchiba A, Liao X, Qian ZR, et al. Physical activity, tumor PTGS2 expression, and survival in patients with colorectal cancer. Cancer Epidemiology and Prevention Biomarkers. 2013.
  24. Adams GR, McCue SA. Localized infusion of IGF-I results in skeletal muscle hypertrophy in rats. Journal of Applied Physiology. 1998;84(5):1716-22.
  25. Karbasi S, Zaeemi M, Mohri M, Rashidlamir A, Moosavi Z. Effects of testosterone enanthate and resistance training on myocardium in Wistar rats; clinical and anatomical pathology. Andrologia. 2017.
  26. Sukho L, Roger F. Resistance training, muscle mass and function in the rats. Journal of Exercise Physiology. 2003;62:80-7.
  27. Attardi LD. The role of p53-mediated apoptosis as a crucial anti-tumor response to genomic instability: lessons from mouse models. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 2005;569(1):145-57.
  28. Stoehlmacher J, Lenz H-J, editors. Cyclooxygenase-2 inhibitors in colorectal cancer. Seminars in oncology; 2003: Elsevier.
  29. Mogharnasi M, Gaeini A, Vatani DS, Faraji H. Effects of aerobic and anaerobic training on inflammatory markers in rats. -. 2011;64(1):21-30.
  30. Hansen AH, Nyberg M, Bangsbo J, Saltin B, Hellsten Y. Exercise training alters the balance between vasoactive compounds in skeletal muscle of individuals with essential hypertension. Hypertension. 2011;58(5):943-9.
  31. OLIVEIRA FD, Maifrino L, JESUS GPD, Carvalho JG, Marchon C, Ribeiro DA. The role of cyclooxygenase-2 on endurance exercise training in female LDL-receptor knockout ovariectomized mice. Anais da Academia Brasileira de Ciências. 2013;85(3):1157-64.
  32. Barari A, Mojhde M, Farzanegi P, Ghasemi M. Effect of Six Weeks of Endurance Training and Aloe Vera on COX-2 and MMP-9 Levels in Mice with Breast Cancer. SSU_Journals. 2016;24(1):65-73.
  33. Renna NF, Diez ER, Lembo C, Miatello RM. Role of Cox-2 in vascular inflammation: an experimental model of metabolic syndrome. Mediators of inflammation. 2013;2013.
  34. Abbasali Gaeini, Aida Bahramian, Mohsen Javidi. The effect of eight weeks of resistance training on stimulatory and inhibitory factors of cardiac microvascular injuries in wistar diabetic rat. Exercise Metabolism1392;3(1):32-21.
  35. Krüger K, Bredehöft J, Mooren FC, Rummel C. Different effects of strength and endurance exercise training on COX-2 and mPGES expression in mouse brain are independent of peripheral inflammation. Journal of Applied Physiology. 2016;121(1):248-54.
  36. Dashzeveg N, Taira N, Lu Z, Kimura J, Yoshida K. Palmdelphin, a novel target of p53 with Ser46 phosphorylation, controls cell death in response to DNA damage. Cell Death and Disease. 2014;5:e1221.
  37. Qi Z, He J, Zhang Y, Shao Y, Ding S. Exercise training attenuates oxidative stress and decreases p53 protein content in skeletal muscle of type 2 diabetic Goto-Kakizaki rats. Free Radical Biology and Medicine. 2011;50(7):794-800.
  38. Dashtiyan A A, Afzalpour M E, Tanideh N, Sepehrimanesh M. The comparison of the effect of vitamin E on the expression of p53/PTEN of prostate gland of male rats in two groups of intensive continuous and intermittent exercise training. Journal Fasa University Medical Scinces. 2017; 7 (3) :406-415
  39. Sharafi H, Rahimi R. The effect of resistance exercise on p53, caspase-9, and caspase-3 in trained and untrained men. The Journal of Strength & Conditioning Research. 2012;26(4):1142-8.
  40. Liu B, Chen Y, Clair DKS. ROS and p53: a versatile partnership. Free Radical Biology and Medicine. 2008;44(8):1529-35.
  • Receive Date: 16 January 2018
  • Revise Date: 07 February 2021
  • Accept Date: 31 December 2020
  • First Publish Date: 31 December 2020
  • Publish Date: 20 February 2019