Effect of Endurance Training on miR-196a and miR-133a Expression in Subcutaneous White Adipose Tissue of Wistar Rats

Document Type : original article

Authors

1 Department of Physical Education, University of Ayatollah Alozma Boroujerdi

2 Department of exercise physiology, Faculty of Physical Education and Sports Sciences,Tehran University

3 Department of Biochemistry, Pasteur Institute of Iran

4 Department of exercise physiology; Faculty of Physical Education and Sports Sciences, Tehran University

Abstract

Purpose: The aim of current study was to investigate the effect of endurance training on expression of miR-196a and miR-133a in subcutaneous white adipose tissue (WAT).
Methods: 16 wistar rats were divided into two groups included: 1) Control (n=8) and 2) Endurance Training (n=8). The subjects of training group underwent continues endurance training on treadmill for eight weeks. To measure of gene expression were used the Real Time (RT) –PCR method.
Results: Data showed that gene expression of UCP1 was significantly higher in trained group than control (P0.05).
Conclusions: The results of this study showed that the endurance training did not change the expression of miR-196a and miR-133a in subcutaneous WAT, indicating that the endurance training-induced the increase of UCP1 expression did not relate to change of miR-196a and miR-133a expression.

Keywords

References

  1. Spiegelman BM, Flier JS. Obesity and the regulation of energy balance. Cell. 2001;104(4):531-43.
  2. Donahoo WT, Levine JA, Melanson EL. Variability in energy expenditure and its components. Curr Opin Clin Nutr. 2004;7(6):599-605.
  3. Dalgaard LT, Pedersen O. Uncoupling proteins: functional characteristics and role in the pathogenesis of obesity and Type II diabetes. Diab tologia. 2001;44(8):946-65.
  4. Schrauwen P, Walder K, Ravussin E. Human uncoupling proteins and obesity. Obes Res. 1999;7(1):97-105.
  5. Arechaga I, Ledesma A, Rial E. The mitochondrial uncoupling protein UCP1: a gated pore. IUBMB life. 2001;52(3-5):165-73.
  6. Ricquier D. Uncoupling protein 1 of brown adipocytes, the only uncoupler: a historical perspective. Frontiers in endocrinology. 2011;2:85.
  7. Walden TB. Regulatory factors that reveal three distinct adipocytes : the brown, the white and the brite. Stockholm: The Wenner-Gren Institute, Stockholm University, 2010.
  8. Walden TB, Hansen IR, Timmons JA, Cannon B, Nedergaard J. Recruited vs. nonrecruited molecular signatures of brown,“brite,” and white adipose tissues. Am J Physiol-Endoc M. 2012;302(1):E19-E31.
  9. Sluse FE, Jarmuszkiewicz W, Navet R, Douette P, Mathy G, Sluse-Goffart CM. Mitochondrial UCPs: new insights into regulation and impact. Biochim Biophys Acta. 2006;1757(5-6):480-5.
  10. Dalgaard L, Pedersen O. Uncoupling proteins: functional characteristics and role in the pathogenesis of obesity and Type II diabetes. Diabetologia. 2001;44(8):946-65.
  11. Kopecky J, Clarke G, Enerbäck S, Spiegelman B, Kozak L. Expression of the mitochondrial uncoupling protein gene from the aP2 gene promoter prevents genetic obesity. J Clin Invest. 1995;96(6):2914.
  12. Feldmann HM, Golozoubova V, Cannon B, Nedergaard J. UCP1 ablation induces obesity and abolishes diet-induced thermogenesis in mice exempt from thermal stress by living at thermoneutrality. Cell metabolism. 2009;9(2):203-9.
  13. Kozak LP, Harper ME. Mitochondrial uncoupling proteins in energy expenditure. Annu Rev Nutr. 2000;20:339-63.
  14. Brondani LA, Assmann TS, Duarte GC, Gross JL, Canani LH, Crispim D. The role of the uncoupling protein 1 (UCP1) on the development of obesity and type 2 diabetes mellitus. Arq Bras Endocrinol Metabol. 2012;56(4):215-25.
  15. Bonet ML, Oliver P, Palou A. Pharmacological and nutritional agents promoting browning of white adipose tissue. Biochim Biophys Acta. 2013;1831(5):969-85.
  16. Wu J, Cohen P, Spiegelman BM. Adaptive thermogenesis in adipocytes: is beige the new brown? Genes Dev. 2013;27(3):234-50.
  17. Ringholm S, Grunnet Knudsen J, Leick L, Lundgaard A, Munk Nielsen M, Pilegaard H. PGC-1alpha is required for exercise- and exercise training-induced UCP1 up-regulation in mouse white adipose tissue. PloS one. 2013;8(5):e64123.
  18. Knudsen JG, Murholm M, Carey AL, Bienso RS, Basse AL, Allen TL, et al. Role of IL-6 in exercise training- and cold-induced UCP1 expression in subcutaneous white adipose tissue. PloS one. 2014;9(1):e84910.
  19. Bostrom P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, et al. A PGC1-alpha-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature. 2012;481(7382):463-8.
  20. Stanford KI, Middelbeek RJW, Goodyear LJ. Exercise Effects on White Adipose Tissue: Beiging and Metabolic Adaptations. Diabetes. 2015;64(7):2361-8.
  21. Xu X, Ying Z, Cai M, Xu Z, Li Y, Jiang SY, et al. Exercise ameliorates high-fat diet-induced metabolic and vascular dysfunction, and increases adipocyte progenitor cell population in brown adipose tissue. American journal of physiology Regulatory, integrative and comparative physiology. 2011;300(5):R1115-25.
  22. Reisi j, H R, K G, S-M M, M-R D. Effect of 8 weeks resistance training on plasma irisin protein level and muscle FNDC5 and adipose tissue UCP1 genes expression in male rats. Sport Physiology. 2016;7(28):117-30.
  23. Reisi J, Ghaedi K, Rajabi H, Marandi SM. Can Resistance Exercise Alter Irisin Levels and Expression Profiles of FNDC5 and UCP1 in Rats? Asian J Sports Med.2016;7(4):e35205.
  24. Daneshyar S, Kordi MR, Gaeini AA, Kadivar M, Afshari S. The Effect of Endurance Training on Gene Expression of Uncoupling Protein 1(UCP-1) in Retroperitoneal White Adipose Tissue of Male Wistar Rats. Razi Journal of Medical Sciences. 2015;22(136):35-45.
  25. Daneshyar S, Kordi MR, Gaeini AA, Kadivar M, Afshari S. The Effect of Endurance Training on Gene Expression of Uncoupling Protein 1(UCP-1) in Retroperitoneal White Adipose Tissue of Male Wistar Rats. Razi Journal of Medical Sciences. 2015;22(136):35-45.
  26. Bostrom P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, et al. A PGC1-alpha-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature. 2012;481(7382):463-8.
  27. Ketting RF. microRNA Biogenesis and Function. Regulation of microRNAs: Springer; 2010. p. 1-14.
  28. Zhou JY, Li L. MicroRNAs are key regulators of brown adipogenesis. Biochim Biophys Acta. 2014;1841(11):1590-5.
  29. Hilton C, Neville MJ, Karpe F. MicroRNAs in adipose tissue: their role in adipogenesis and obesity. Int J Obes (Lond). 2013;37(3):325-32.
  30. Chen J, Deng S, Zhang S, Chen Z, Wu S, Cai X, et al. The role of miRNAs in the differentiation of adipose-derived stem cells. Curr Stem Cell Res T. 2014;9(3):268-79.
  31. Trajkovski M, Lodish H. MicroRNA networks regulate development of brown adipocytes. Trends in endocrinology and metabolism: TEM. 2013;24(9):442-50.
  32. Mori M, Nakagami H, Rodriguez-Araujo G, Nimura K, Kaneda Y. Essential role for miR-196a in brown adipogenesis of white fat progenitor cells. PLoS biology. 2012;10(4):e1001314.
  33. Trajkovski M, Ahmed K, Esau CC, Stoffel M. MyomiR-133 regulates brown fat differentiation through Prdm16. Nat Cell Biol. 2012;14(12):1330-5.
  34. Liu W, Bi P, Shan T, Yang X, Yin H, Wang Y-X, et al. miR-133a regulates adipocyte browning in vivo. PLoS Genet. 2013;9(7):e1003626.
  35. De Matteis R, Lucertini F, Guescini M, Polidori E, Zeppa S, Stocchi V, et al. Exercise as a new physiological stimulus for brown adipose tissue activity. Nutrition, metabolism, and cardiovascular diseases : NMCD. 2013;23(6):582-90.
  36. Kraemer WJ, Rogol AD. The Endocrine System in Sports and Exercise. International Olympic Committee, 2006.
  37. Zouhal H, Jacob C, Delamarche P, Gratas-Delamarche A. Catecholamines and the effects of exercise, training and gender. Sports Med. 2008;38(5):401-23.
  38. Cousineau D, De Champlain J, Nadeau R. Plasma norepinephrine response to exercise before and after training in humans. Journal of Applied Physiology.1981;51(4): 812-815
  39. Rosell S, Belfrage E. Blood circulation in adipose tissue. Physiol Rev. 1979;59(4):1078-104.
  40. Berkowitz DE, Nardone NA, Smiley RM, Price DT, Kreutter DK, Fremeau RT, et al. Distribution of β 3-adrenoceptor mRNA in human tissues. Eur J Pharmacol. 1995;289(2):223-8.

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History

  • Receive Date: 14 May 2017
  • Revise Date: 07 February 2021
  • Accept Date: 31 December 2020
  • First Publish Date: 31 December 2020
  • Publish Date: 20 February 2019

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