Effect of acute and high- intensity interval training period in normal and hot conditions on serum levels of glutathione peroxidase and HSP72 in active men

Document Type : original article

Abstract



Aim: Anti oxidative enzymes and heat shock protein72 (HSP72) are two important internal body protective
factors. The purpose of present study was to determine the effects of 1 and 12 sessions of high intensity
interval training in warm and normal environment on serum levels of Glutathione peroxidase and HSP72 in
active men (at least 8 hours per week). Methodology: Twenty four physical education students of Kharazmi
university were divided into three equal and homogenous groups include: exercise in warm
environment(temperature 40± 1, humidity 35 ± 5),exercise in normal environment(temperature 24, humidity
35 ±5) and control group. Training protocol included running on treadmill with 85% vVO2max intensity in 5
sets per 150 second. Training sets separated from each others by running with 50% vVO2max intensity as a
recovery time (150 second). Blood samples were taken before and after first activity session and 24 hours
after last training session and Glutathione peroxidase (GPX) and HSP72 levels measured via ELISA method.
Data was analyzed using the independent and dependent t tests and one way ANOVA at significance level of
p<0.05. Results: Serum levels of GPX and HSP72 increased significantly after 1 and 12 sessions of high
intensity interval training compared to pre-test in both of warm and normal environment groups. However any
between group significant differences was not observed in these two groups . Furthermore, after 12 session of
practice the serum level of GPX in both warm and normal environment training groups increased significantly
in comparison with control group, but the serum level of HSP72 was not significant in two training groups
when compared with control group. Conclusion: In general result of present study showed that 1 session and
1 high intensity interval training period is stimulator of GPX and HSP72 and arising the environmental
temperature has no effect on response and adaptation values of mentioned factors.

Keywords

References

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Journal of Sport and Exercise Physiology
Volume 8, Issue 1 - Serial Number 15
February 2016
Pages 1201-1212

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History

  • Receive Date: 09 February 2016
  • Revise Date: 11 June 2024
  • Accept Date: 31 December 2020
  • First Publish Date: 31 December 2020
  • Publish Date: 21 April 2015

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