The effects of short-term creatine monohydrate supplementation on a single resistance exercise induced-indirect indicators some of cellular damage in male wrestler

Purpose: Creatine monohydrate as one of the most widely consumed nutritional supplements to increase
athletic performance is widely popular among athletes. However, there are recent concerns about some of
the adverse effects of short and long term use of creatine on health indices. Therefore this study was
conducted to identify the effect of creatine monohydrate loading on total serum cellular damage indices
changes (Creatine Kinase & Lactate Dehydrogenase) following one session resistance exercise in male
wrestler. Methods: Eighteen volunteer collegiate young male wrestlers (aged 21.11±1.13 years, fat
12.67±1.84 %, and BMI 21.96±0.70 kg/m2) in a quasi-experimental, randomized and double-blind design
were allocated equally into supplement and placebo groups. After six consecutive days supplementation
(300 mg.kg-1.day body weight creatine monohydrate or dextrose), all subjects were participated in a
single- session circle resistance exercise protocol (with 80 % 1-RM in 3 sets with 6 repetition) include six
station participated. Changes in cellular damage indices were determined in three phases (before and after
the supplement phase, 24 hours after the exercise protocol). Data were analyzed by repeated measure
ANOVA, Bonferroni and independent t test at α≤0.05. Results: The results show that the creatine loading
has only significant effect on the basal total serum CK in supplement group (P<0.05). Moreover, the total
serum CK and LDH in both groups (supplement & placebo) were significantly increased 24 hour after the
resistance exercise (P<0.05). However, the change range of the cellular damage indices 24 hours in
supplement group was insignificantly lower than in placebo group (P>0.05).Conclusion: According to
normal variation of total serum Creatine Kinase in the base case and due to the lack of effect of creatine
on 24 hours indicators of cellular damage in male wrestler after the resistance exercise, definitive
comment in related creatine supplementation induced- cellular damage, more research is needed in the
further.