The Effect of high intensity interval training on serum levels of Osteopontin and insulin resistance index in sedentary overweight and obese women

Document Type : original article

Authors

1 Department of Physical Education and Sport Sciences, Faculty of Humanities, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran

2 Department of Physical Education and Sports Science, Faculty of Educational Sciences and Psychology, Shiraz University, Shiraz, Iran

Abstract

Purpose: Osteopontin is a novel adipose tissue derived cytokine correlating with obesity and its related metabolic diseases such as insulin resistance and type II diabetes. The purpose of this study was to investigate effect of high intensity interval training (HIIT) on serum levels of Osteopontin, insulin and fasting glucose and insulin resistance index (HOMA-IR) in young sedentary overweight and obese women.
Methods: Twenty overweight and obese women randomly divided into two groups including experimental (mean age 32.5 ± 4.14 years, body mass index 33.18 ± 6.12 Kg/m2) and control (mean age 35.45 ± 2.33 years, body mass index 31.58 ± 1.65 Kg/m2). Subjects in experimental group participated in eight weeks of HIIT (one minute of running interval at intensity of 90 percent of maximal heart rate and one minutes of rest interval of running at intensity of 50-60 percent of maximal heart rate, 10 sets per session, 20 minutes per session, three times per week). Blood factors and body composition indices were assessed before and after training protocol. Statistical analysis was done by Covariance, paired t-test and Pearson correlation at significant level of P < 0.05.
Results: According to paired t-test, HIIT resulted in significant decrease in serum levels of osteopontin (P = 0.0001) and weight (P = 0.0001), body mass index (P = 0.0001) and body fat percent (P = 0.0001) in post-test compared to pre-test. However, Covariance showed that only changes in levels of insulin (P = 0.039), weight (P = 0.001), body mass index (P = 0.005) and body fat percent (P = 0.0001) were significantly different between training and control groups. Moreover, there were negative correlation between changes in osteopontin level and changes in body mass index (P = 0.0001) and body fat percent (P = 0.044).
Conclusion: High intensity interval training (HIIT) decreased osteopontin level and improved body composition and metabolic status, and therefore, can be suggested as a therapeutic procedure in obese and overweight young women.

Keywords

References

  1. Pradeep AR, Priyanka N, Prasad MV, Kalra N, Kumari M. Association of progranulin and high sensitivity CRP concentrations in gingival crevicular fluid and serum in chronic periodontitis subjects with and without obesity. Disease markers. 2012;33(4):207-13.
  2. You JS, Ji HI, Chang KJ, Yoo MC, Yang HI, Jeong IK, Kim KS. Serum osteopontin concentration is decreased by exercise-induced fat loss but is not correlated with body fat percentage in obese humans. Molecular medicine reports. 2013;8(2):579-84.
  3. Kahles F, Findeisen HM, Bruemmer D. Osteopontin: A novel regulator at the cross roads of inflammation, obesity and diabetes. Molecular metabolism. 2014;3(4):384-93.
  4. Komorowski J, Jankiewicz-Wika J, Kolomecki K, Cywinski J, Piestrzeniewicz K, Swiętoslawski J, Stepien H. Systemic blood osteopontin, endostatin, and E-selectin concentrations after vertical banding surgery in severely obese adults. Cytokine. 2011;55(1):56-61.
  5. Pagel CN, Wijesinghe DK, Esfandouni NT, Mackie EJ. Osteopontin, inflammation and myogenesis: influencing regeneration, fibrosis and size of skeletal muscle. Journal of cell communication and signaling. 2014;8(2):95-103.
  6. Kiefer FW, Zeyda M, Todoric J, Huber J, Geyeregger R, Weichhart T, Aszmann O, Ludvik B, Silberhumer GR, Prager G, Stulnig TM. Osteopontin expression in human and murine obesity: extensive local up-regulation in adipose tissue but minimal systemic alterations. Endocrinology. 2008 ;149(3):1350-7.
  7. Kiefer FW, Zeyda M, Gollinger K, Pfau B, Neuhofer A, Weichhart T, Säemann MD, Geyeregger R, Schlederer M, Kenner L, Stulnig TM. Neutralization of osteopontin inhibits obesity-induced inflammation and insulin resistance. Diabetes. 2010;59(4):935-46.
  8. Abedi & Ekhvat. The effect of eight weeks of high intensity interval training (HIIT) on serum adiponectin levels and insulin resistance in type 2 diabetic women. Journal of Sport Biological Sciences. 2016; 8(3).
  9. Sigal RJ, Kenny GP, Wasserman DH, Castaneda-Sceppa C, White RD. Physical activity/exercise and type 2 diabetes: a consensus statement from the American Diabetes Association. Diabetes care. 2006;29(6):1433-8.
  10. Trapp EG, Chisholm DJ, Freund J, Boutcher SH. The effects of high-intensity intermittent exercise training on fat loss and fasting insulin levels of young women. International journal of obesity. 2008;32(4):684-91.
  11. Little JP, Jenna B, Gillen ME, Percival AS, Mark A, Tarnopolsky ZP, Mary EJ, Martin JG. Low-volume high-intensity interval training reduces hyperglycemia and increases muscle itochondrial capacity in patients with type 2 diabetes. Journal of Applied Physiology 2011; 11(6): 1554-1560
  12. Grace F, Herbert P, Elliott AD, Richards J, Beaumont A, Sculthorpe NF. High intensity interval training (HIIT) improves resting blood pressure, metabolic (MET) capacity and heart rate reserve without compromising cardiac function in sedentary aging men. Experimental Gerontology. 2018;109:75-81.
  13. Barfield WL, Uaesoontrachoon K, Wu CS, Lin S, Chen Y, Wang PC, Kanaan Y, Bond V, Hoffman EP. Eccentric muscle challenge shows osteopontin polymorphism modulation of muscle damage. Human molecular genetics. 2014;23(15):4043-50.
  14. Verheggen RJ, Poelkens F, Roerink SH, Ramakers RE, Catoire M, Hermus AR, Thijssen DH, Hopman MT. Exercise Improves Insulin Sensitivity in the Absence of Changes in Cytokines. Med Sci Sports Exerc. 2016; 48(12):2378-2386
  15. Hunter M. The women’s health questionnaire: a measure of mid-aged women’s perceptions of their emotional and physical health. Psychol Health 1992; 7 (1): 45-54.
  16. Hagströmer M, Oja P, Sjöström M. The International Physical Activity Questionnaire (IPAQ): a study of concurrent and construct validity. Public Health Nutr 2006; 9(6):755-62.
  17. Tanaka H, Monahan KD, Seals DR. Age-predicted maximal heart rate revisited. Journal of the american college of cardiology. 2001;37(1):153-6.
  18. Akima H, Takahashi H, Kuno Sy, Masuda K, Masuda T, Shimojo H, Anno I, Itai Y, Katsuta S. Early phase adaptations of muscle use and strength to isokinetic training. Medicine & Science in Sports & Exercise. 1999;31(4):588-94.
  19. Gillen JB, Percival ME, Ludzki A, Tarnopolsky MA, Gibala MJ. Interval training in the fed or fasted state improves body composition and muscle oxidative capacity in overweight women. Obesity. 2013;21(11):2249-55.
  20. Mathews ST, Singh GP, Ranalletta M, Cintron VJ, Qiang X, Goustin AS, Jen KL, Charron MJ, Jahnen-Dechent W, Grunberger G. Improved insulin sensitivity and resistance to weight gain in mice null for the Ahsg gene. Diabetes. 2002;51(8):2450-8.
  21. Abbaszadeh Sorati, Hajar; Ebrahim, Khosrow; Nikbakht, Hojatollah; Effect of 16 weeks of selected aerobic training on serum osteopontin and osteocalcin in inactive middle-aged women. Journal of Exercise Physiology and Physical Activity. No. 8, 2012.
  22. Pagel CN, Wijesinghe DKW, Taghavi Esfandouni N, Mackie EJ. Osteopontin, inflammation and myogenesis: influencing regeneration, fibrosis and size of skeletal muscle. Journal of Cell Communication and Signaling. 2014; 8(2): 95–103.
  23. Hoffman EP, Gordish-Dressman H, McLane VD, Devaney MJ, Thompson PD, Visich P, Gordon PM, Pescatello LS, Zoeller RF, Moyna NM, Angelopoulos TJ, Pegoraro E, Cox GA, Clarkson PM. Alterations in Osteopontin Modify Muscle Size in Females in Both Humans and Mice. Medicine and Science in Sports and Exercise. 2013; 45(6): 1060–1068.
  24. Barfield WL, Uaesoontrachoon K, Wu C-Sh, Lin S, Chen Y, Wang PC, Kanaan Y, Bond V, Hoffman EP. Eccentric muscle challenge shows osteopontin polymorphism modulation of muscle damage. Human Molecular Genetics. 2014; 23(15): 4043-50.
  25. Duggan C, Xiao L, Wang CY, McTiernan A. Effect of a 12-month exercise intervention on serum biomarkers of angiogenesis in postmenopausal women: a randomized controlled trial. Cancer epidemiology, biomarkers & prevention: a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology. 2014;23(4):648-57.
  26. Venojarvi M, Korkmaz A, Wasenius N et al. 12 weeks' aerobic and resistance training without dietary intervention did not influence oxidative stress but aerobic training decreased atherogenic index in middle-aged men with impaired glucose regulation. Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association. 2013; 61:127-35.
  27. Verheggen RJHM, Poelkens F, Roerink SHGPP, Ramakers REFS, Catoire M, Hermus ARMM, Thijssen DHJ, Hopman MTE. Exercise Improves Insulin Sensitivity in the Absence of Changes in Cytokines. Medicine and Science in Sports and Exercise. 2016; 48(12):2378-2386
  28. Abbasi T, Nazarali P, Hedayati M, Alizadeh R. The effect of eight weeks of high intensity interval training on osteoponetin and some bone mineral indices in young women. Journal of Physical Education and Sport. 2018, 18, 532–535.
  29. Chapman J, Miles PD, Ofrecio JM, Neels JG, Yu JG, Resnik JL, Wilkes J, Talukdar S, Thapar D, Johnson K, Sears DD. Osteopontin is required for the early onset of high fat diet-induced insulin resistance in mice. PloS one. 2010;5(11).
  30. Church T, Barlow C, Earnest C. Associations between cardio respiratory fitness and C-reactive protein in men. Arteriosclerosis, Thrombosis, and Vascular Biology. 2002;22(1):1869-76.
  31. Balamatsias D, Kong AM, Waters JE, Sriratana A, Gurung R, Bailey CG, et al. Identification of P-Rex1 as a novel Rac1-guanine nucleotide exchange factor (GEF) that promotes actin remodeling and GLUT4 protein trafficking in adipocytes. Journal of Biological Chemistry. 2011;286(50):43229-40.
  32. Samuel VT, Shulman GI. Mechanisms for insulin resistance: common threads and missing links. Cell. 2012;148(5): 852-71.
  33. Chiu T, Patel N, Shaw AE, Bamburg JR, Klip A .Arp2/3-and cofilin-coordinated actin dynamics is required for insulinmediated GLUT4 translocation to the surface of muscle cells. Molecular biology of the cell. 2010;21(20):3529-39
  34. Keshel TE, Coker RH. Exercise Training and Insulin Resistance: A Current Review. J Obes Weight Loss Ther. 2015; 5(0 5): S5-003.
  35. Henriksen EJ. Invited review: Effects of acute exercise and exercise training on insulin resistance. J Appl Physiol (1985). 2002; 93(2):788-96.
  36. Denhardt DT, Mistretta D, Chambers AF, Krishna S, Porter JF, Raghuram S, Rittling SR. Transcriptional regulation of osteopontin and the metastatic phenotype: evidence for a Ras-activated enhancer in the human OPN promoter. Clinical & experimental metastasis. 2003;20(1):77-84.
  37. Samuvel DJ, Sundararaj KP, Li Y, Lopes-Virella MF, Huang Y. Adipocyte-mononuclear cell interaction, Toll-like receptor 4 activation, and high glucose synergistically up-regulate osteopontin expression via an interleukin 6-mediated mechanism. Journal of Biological Chemistry. 2010;285(6):3916-27.
  38. Lu C, Kumar PA, Sun J, Aggarwal A, Fan Y, Sperling MA, Lumeng CN, Menon RK. Targeted deletion of growth hormone (GH) receptor in macrophage reveals novel osteopontin-mediated effects of GH on glucose homeostasis and insulin sensitivity in diet-induced obesity. Journal of Biological Chemistry. 2013;288(22):15725-35.
  39. Omar B, Banke E, Guirguis E, Åkesson L, Manganiello V, Lyssenko V, Groop L, Gomez MF, Degerman E. Regulation of the pro-inflammatory cytokine osteopontin by GIP in adipocytes–a role for the transcription factor NFAT and phosphodiesterase 3B. Biochemical and biophysical research communications. 2012; 425(4):812-7.
  40. Ahlqvist E, Osmark P, Kuulasmaa T, Pilgaard K, Omar B, Brøns C, Kotova O, Zetterqvist AV, Stančáková A, Jonsson A, Hansson O. Link between GIP and osteopontin in adipose tissue and insulin resistance. Diabetes. 2013; 62(6):2088-94.
  41. Zeyda M, Gollinger K, Todoric J, Kiefer FW, Keck M, Aszmann O, Prager G, Zlabinger GJ, Petzelbauer P, Stulnig TM. Osteopontin is an activator of human adipose tissue macrophages and directly affects adipocyte function. Endocrinology. 2011; 152(6):2219-27.
  42. De Fusco C, Messina A, Monda V, Viggiano E, Moscatelli F, Valenzano A, Esposito T, Sergio C, Cibelli G, Monda M, Messina G. Osteopontin: Relation between Adipose Tissue and Bone Homeostasis. Stem Cells Int. 2017; 2017:4045238.
Journal of Sport and Exercise Physiology
Volume 14, Issue 2 - Serial Number 27
January 2022
Pages 115-126

Files

History

  • Receive Date: 21 April 2020
  • Revise Date: 02 May 2021
  • Accept Date: 12 May 2021
  • First Publish Date: 23 September 2021
  • Publish Date: 23 September 2021

Share

How to cite

Statistics