Comparison of the effects of 10 weeks mid and long-term swimming induced oxidative stress on left ventricular angiogenesis in male rats

Document Type : original article

Authors

1 Department of Sports Science, Faculty of Humanities, Damghan University, Damghan, Iran

2 Faculty of Sports Sciences, Bu Ali Sina University, Hamadan, Iran

Abstract

Purpose: In previous studies, there was a relationship between angiogenesis and oxidative stress thus the aim of this study was to compare the effect of two protocols of mid and long-term on left ventricular angiogenesis in male rats.
Methods:  The 18 male Wistar rats of 210 ± 20g, were randomly assigned in to three equal groups: control, mid and long-term group. Training groups swam in 32֯ water five days per week for 10 weeks. The mid-term group swam for one hour and the long-term group for three hours per session. One day after the end of the protocol, left ventricle of the heart was removed. Real-time PCR method was used to measure the genes expression of Vascular-endothelial-growth-factor-B, Fibroblast-Growth-Factor-B, Angiopoiteine1&2. Differences were determined using by ANOVA One-Way method and the groups were compared by Tukey follow-up test at the significant level of P < 0.05.
Results: The genes expression levels of Vascular-endothelial-growth factor-B, Angiopoiteine1&2 and Matrix-metalloproteinases-2 were significantly increased in the long-term group in compared to the both the control and mid-term groups, and in the mid-term group in compared to the control group, but the gene expression of Fibroblast-Growth-Factor-B had a significant increase only in the mid-term group in compared to the control group (P = 0.001).
Conclusion: Due to the significantly increase of nitric-oxide, the angiogenesis process in the long-term group can be related to shear stress, and in the mid-term group, due to a significant increase in Malondialdehyde, it was attributed to oxidative stress.

Keywords

References

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History

  • Receive Date: 02 January 2019
  • Revise Date: 03 March 2019
  • Accept Date: 07 June 2020
  • First Publish Date: 22 June 2021
  • Publish Date: 22 June 2021

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