تأثیر چهار هفته تمرین تناوبی شدید ) HIIT ( بر محتوای پروتئینهای 1 ،AKT4EBP ،mTOR، P7 0S6K در بافت عضلانی بطن چپ قلب موشهای صحرای چاق مبتلا به دیابت نوع 2

آقایی بهمن بگلو, ندا; شادمهری, سعیده; جهانی گلبر, شیوا; شرافتی, محمد

doi:10.52547/joeppa.14.1.85

تأثیر چهار هفته تمرین تناوبی شدید ) HIIT ( بر محتوای پروتئینهای 1 ،AKT4EBP ،mTOR، P7 0S6K در بافت عضلانی بطن چپ قلب موشهای صحرای چاق مبتلا به دیابت نوع 2

نوع مقاله : علمی - پژوهشی

نویسندگان

¹ گروه تربیت بدنی و علوم ورزشی، واحد علی‌آباد کتول، دانشگاه آزاد اسلامی، علی آباد کتول، ایران

² استادیار، گروه تربیت بدنی و علوم ورزشی، واحد یادگار امام خمینی ( ره ) شهر ری، دانشگاه آزاد اسلامی، تهران، ایران

³ گروه تربیت بدنی و علوم ورزشی، واحد یادگار امام خمینی ( ره ) شهر ری، دانشگاه آزاد اسلامی، تهران، ایران

⁴ گروه علوم ورزشی، مؤسسة آموزش عالی آپادانا، شیراز، ایران

10.52547/joeppa.14.1.85

چکیده

هدف: مسیر کمپلکس یک هدف راپامایسین در پستانداران (mTORC1) از مسیرهای مهم سنتز پروتئین در قلب است که بیماری دیابت می‌تواند به اختلال در این مسیر منجر شود. هدف از مطالعة حاضر، بررسی تأثیر 4 هفته تمرین تناوبی شدید (HIIT) بر محتوای پروتئین‌های AKT1، mTOR، P70S6K1، 4EBP1 در بافت عضلانی بطن چپ قلب موش‌های صحرایی چاق مبتلا به دیابت نوع 2 است.
روش‌ها: در این مطالعه، 16 سر موش صحرایی نر نژاد اسپراگوداولی (با میانگین وزن20±300 گرم) انتخاب و پس از دیابتی شدن از طریق القای STZ و نیکوتین‌آمید به روش تصادفی به دو گروه تمرین دیابتی و کنترل دیابتی تقسیم شدند؛ گروه تجربی 4 روز در هفته مطابق برنامة تمرینی به مدت 4 هفته به تمرین ورزشی HIIT پرداختند؛ درحالی‌که گروه کنترل هیچ‌گونه برنامة تمرینی نداشتند. برای تجزیه‌وتحلیل داده‌ها از آزمون t-مستقل استفاده شد.
نتایج: میان محتوای تام پروتئین‌های AKT1 (56/0=P)، mTOR (50/0=P)، P70S6K1 (99/0=P) و 4E-BP1 (32/0=P) گروه‌های تمرین و کنترل تفاوت معناداری مشاهده نشد. همچنین بین محتوای فسفریله پروتئین‌های AKT1^ser473 (25/0=P)، mTOR^ser2448 (74/0=P)، P70S6K1^Thr389 (37/0=P) و 4E-BP1^Thr37/46 (38/0=P) گروه‌های تمرین و کنترل تفاوت معناداری دیده نشد.
نتیجه‌گیری: برنامة HIIT بر محتوای تام و فسفریله پروتئین‌های AKT1، mTOR، P70S6K1، 4EBP1 بطن چپ قلب موش‌های صحرایی چاق مبتلا به دیابت نوع 2 اثر معناداری ندارد.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of four weeks high-intensity interval training (HIIT) on the content of AKT1, mTOR, P70S6K1, 4EBP1 proteins in the Left ventricular muscle tissue of the heart obese rats with type 2 diabetic

نویسندگان [English]

Neda Aghaei Bahman Beglou ¹
saeedeh shadmehri ²
Shiva Jahani Golbar ³
Mohammad Sharafati Moghadam ⁴

¹ 1Department of Physical Education and Sport Sciences, AliabadKatoul Branch, Islamic Azad University, AliabadKatul, Iran.

² Islamic Azad University

³ Department of Physical Education and Sport Sciences, Yadegar-e-imam Khomeini (RAH) Shahr-e Ray Branch, Islamic Azad University, Tehran, Iran

⁴ Department of Sports Science, Apadana Higher Education Institute, Shiraz, Iran

چکیده [English]

Purpose: The pathway for a Rapamycin target in mammals (mTORC1) is one of the important pathways for protein synthesis in the heart, and diabetes can lead to disorder of this pathway. The aim of this study was to investigate the effect of four weeks high-intensity interval training (HIIT) on the content of AKT1, mTOR, P70S6K1, 4EBP1 proteins in the left ventricular muscle tissue of the heart obese rats with type 2 diabetic.
Methods: In this experimental study, 16 Sprague-Dawley male rats (with mean weight of 300 ± 20 gr) were selected and after induction of diabetes by STZ and nicotinamide was randomly assigned into two groups: diabetic training and diabetic control. The experimental group performed HIIT training for four weeks’ accordance with the training program for four weeks, while the control group did not have any training program. Independent t-test was used to analyze the data.
Results: There was no significant difference change the total form content of the AKT1 proteins (p=0.56), mTOR (p=0.50), P70S6K1 (p=0.99) and 4E-BP1 (p=0.32) in the training group compared to the control group; Also, the HIIT training did not significantly changed the phosphorylated form content of AKT1^ser473 (p=0.25), mTOR^ser2448 (p=0.74), P70S6K1^Thr389 (p=0.37) and 4E-BP1^Thr37/46(p=0.38) proteins in the training group compared to the control group.
Conclusion: HIIT training has not significantly changed the total and phosphorylated form content of the AKT1, mTOR, P70S6K1, 4EBP1 proteins in the left ventricle of heart tissue obese rats with type 2 diabetic.

کلیدواژه‌ها [English]

High-intensity interval training
Heart tissue
mTORC1 pathway
Type 2 diabetes

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