تأثیر تعاملی تمرین شنا و مکمل کراتین بر مقاومت انسولینی و تحمل گلوکز موش‌های صحرایی نر چاق

نوع مقاله : علمی - پژوهشی

نویسندگان

1 دانشکدة علوم ورزشی، دانشگاه بوعلی سینا همدان، همدان، ایران

2 کارشناس ارشد فیزیولوژی ورزش، دانشکدة علوم ورزشی، دانشگاه بوعلی سینا همدان، همدان، ایران

چکیده

هدف: تحقیق حاضر به‌منظور بررسی تأثیر مکمل کراتین به‌تنهایی یا در حالت ترکیب با ورزش شنا بر مقاومت انسولینی، تحمل گلوکز و کارایی انسولین موش‌های صحرایی نر چاق انجام گرفت.
روش‌ها: 50 سر موش صحرایی نر نژاد ویستار (15±160 گرم)، به‌صورت تصادفی در پنج گروه قرار گرفتند: کنترل، غذای پرچرب، مکمل کراتین، تمرین شنا و مکمل کراتین همراه با تمرین شنا. برنامة تمرین شامل مدت دوازده هفته تمرین شنا بود که هر دو هفته یک‌بار زمان تمرین بیشتر می‌شد. در انتها موش‌های صحرایی تحت آزمون تحمل گلوکز خوراکی (OGTT) قرار گرفتند و نمونه‌های خونی برای سنجش مقادیر TG, HDL و انسولین گرفته شد. همچنین عضلة نعلی طرف راست برای سنجش میزان پروتئین PGC-1α برداشته شد.
نتایج: چاقی به‌تنهایی سبب افزایش مقاومت انسولین و کاهش تحمل گلوکز و کارایی انسولین موش‌های صحرایی شد و مکمل کراتین به‌تنهایی تأثیری بر این تغییرات نداشت. تمرین به‌تنهایی سبب کاهش مقاومت انسولین و افزایش تحمل گلوکز و کارایی انسولین موش‌های صحرایی چاق شد و مکمل کراتین همراه با تمرین تأثیر فزاینده بر تغییرات ذکرشده داشت. چاقی به کاهش بیان پروتئین PGC-1α و تمرین به افزایش سطح این پروتئین منجر شد. مکمل کراتین به‌تنهایی یا با ورزش تأثیری بر بیان این پروتئین نداشت (05/0P<).   
نتیجه‌گیری: نتایج پژوهش حاضر برای اولین بار نشان داد که ترکیب مکمل کراتین و تمرین شنا سبب بهبود کنترل گلایسمیک، کاهش مقاومت انسولین و افزایش کارایی انسولین در موش‌های صحرایی چاق شد. تمرین به افزایش بیان پروتئین PGC-1α انجامید، اما مکمل کراتین بر تغییرات این پروتئین تأثیر نداشت.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of training and creatine supplementation interaction on insulin resistance and glucose tolerance in obese male rats

نویسندگان [English]

  • Hesam Parsa 1
  • Morteza Zareie 2
1 Faculty of Sport Sciences, Bu Ali Sina University, Hamedan, Iran
2 Master of Sports Physiology, Faculty of Sports Sciences, Bu Ali Sina University, Hamedan, Iran
چکیده [English]

Purpose: The present study was undertaken to explore the effects of exercise training and creatine supplementation interaction on insulin resistance and glucose tolerance in obese male rats.
Methods: 50 male Wistar rats (weigh; 160 ± 15 g) were randomly divided into five groups. Control, High-fat Diet, High-Fat Diet plus creatine supplementation, High-Fat Diet plus exercise training and High-Fat Diet plus Creatine plus exercise training. Training program was 12 weeks swimming and each week its duration increased. At the end, rats underwent the glucose tolerance test (OGTT) and the blood samples for analyzing TG, HDL and insulin collected. Moreover, for determining of PGC-1α expression the SOL muscle dissected. 
Results: Obesity resulted in increased insulin resistance level and it also reduced glucose tolerance and insulin efficiency; creatine supplementation alone did not affect these changes. Training reduced insulin resistance and also elevated glucose tolerance and insulin efficiency in high-fat fed rats and creatine supplementation combined with training had additive effect on these variables. High-fat diet reduced PGC-1α protein level and training elevated it. Creatine supplementation alone or combined with training did not change the expression of this protein (P < 0.05).
Conclusion: For the first time, this study shows that combined creatine supplementation with training resulted in improved glycemic control and insulin efficiency and it also reduced the insulin resistance of obese rats.

کلیدواژه‌ها [English]

  • Creatine
  • Swimming
  • Insulin resistance
  • Glucose tolerance
  • High-fat diet
  • Obesity

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سابقه مقاله

  • تاریخ دریافت: 02 دی 1399
  • تاریخ بازنگری: 01 خرداد 1400
  • تاریخ پذیرش: 03 خرداد 1400
  • تاریخ اولین انتشار: 14 آذر 1400
  • تاریخ انتشار: 01 فروردین 1401

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