تأثیر 12 هفته تمرین تناوبی با شدت بالا (HIIT) و مکمل کوآنزیم Q10 بر بیوژنز میتوکندری عضله نعلی موش‌های صحرائی چاق شده با رژیم پرچرب

رفعتی, مسعود; بشیری, جبار; پوزش جدیدی, رقیه; پوررضی, حسن

doi:10.52547/joeppa.15.2.29

تأثیر 12 هفته تمرین تناوبی با شدت بالا (HIIT) و مکمل کوآنزیم Q10 بر بیوژنز میتوکندری عضله نعلی موش‌های صحرائی چاق شده با رژیم پرچرب

نوع مقاله : علمی - پژوهشی

نویسندگان

¹ گروه علوم ورزشی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

² گروه علوم ورزشی، دانشکده علوم اجتماعی، دانشگاه بین‌المللی امام خمینی (ره)، قزوین، ایران

10.52547/joeppa.15.2.29

چکیده

هدف: در مورد تأثیر توأم HIIT و Q10 بر دستکاری بیوژنز و عملکرد میتوکندری‌های عضلانی در شرایط چاقی اطلاعات اندکی موجود است. هدف پژوهش حاضر بررسی اثر توأم تمرین HIIT و مکمل Q10 بر محتوای میتوکندری و مقدار AMPK، NRF2، SIRT1 و Tfam عضله نعلی موش‌های صحرائی نر چاق بود.
روش‌ها: 48 موش صحرائی به ‌طور تصادفی به شش گروه لاغر، چاق مرجع، چاق کنترل، چاق+HIIT، چاق+Q10 و چاق توأم (HIIT+Q10) تقسیم شدند. چاقی با رژیم پرچرب القا شد و تمرین HIIT به مدت 12 هفته (پنج جلسه در هفته، هر جلسه شامل10 مرحله‌ فعالیت تناوبی چهار دقیقهای با شدت 90-85 درصد v VO2 peak) انجام شد و Q10 روزانه (نیم گرم در هر کیلوگرم وزن بدن) مصرف شد. داده‌ها با روش وسترن بلات و mitotracker اندازه‌گیری و با ANOVA مقایسه شدند.
نتایج: محتوای میتوکندری (049/0=P) و مقدار پروتئین‌های NRF2(002/0=P)، SIRT-1(007/0=P) و Tfam(040/0=P) در گروه چاق کنترل به طور معنادار کمتر از گروه لاغر بود. محتوای میتوکندری و مقدار SIRT-1 در هر سه گروه چاق+ HIIT(001/0=P)، چاق+ Q10(001/0=P) و چاق توأم (001/0=P)، به طور معنا‌داری بیشتر از گروه چاق کنترل بود و حتی به سطوح بالاتر از گروه لاغر نیز (به جز محتوای میتوکندری گروه چاق+Q10) رسید (001/0=P). اما مقدار NRF2، فقط در گروه‌های چاق+ HIIT(033/0=P) و چاق توأم (038/0=P) به طور معنا‌داری بیشتر از گروه کنترل چاق بود. بااین‌حال، در هیچ یک از گروه‌های مداخله، مقدار Tfam تفاوت معنا‌داری با گروه کنترل چاق نداشت (05/0
نتیجه گیری: احتمالاً چاقی بیوژنز میتوکندری عضله نعلی را کاهش می‌دهد و یا اینکه حداقل سبب تخریب بیشتر میتوکندری‌های موجود می‌شود، اما تمرین HIIT و مکمل Q10 تا اندازه‌ای قابلیت جبران این کاهش را دارند که البته اثر HIIT تا حدی بیشتر است. بااین‌حال به دلیل کمبود شواهد و محدودیت‌ها هنوز نیاز به بررسی‌ باقی است.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of 12 weeks of HIIT and Q10 supplementation on soleus muscle mitochondrial biogenesis in high-fat diet-induced obese rats

نویسندگان [English]

Masoud Rafati ¹
Jabbar Bashiri ¹
Roghayeh Poozesh Jadidi ¹
Hassan Pourrazi ²

¹ Department of Sports Science, Tabriz Branch, Islamic Azad University, Tabriz, Iran

² Department of Sports Science, Faculty of Social Sciences, Imam Khomeini International University, Qazvin, Iran

چکیده [English]

Purpose: little is known about the concomitant effects of HIIT and Q10 supplementation in modification of the mitochondorial biogenesis and function in obesity conditions. The aim of this study was to investigate the concomitant effects of HIIT and Q10 supplementation on soleus muscle mitochondorial content as well as NRF2, SIRT-1 and Tfam levels in obese male rarts.
Methods: 48 rats randomized into six groups of lean, obese reference, obese control, obese+HIIT, obese+Q10 and obese concomitant (HIIT+Q10). Obesity was induced by high fat diet and HIIT) were done for 12 weeks (five sessions/week, with 10 intrval bouts for four min at 85-90% of v VO2 peak each session), while Q10 was consumed 500 mg/kg.bw daily. Data were measuered using western blot and Mitotrackervmethods and were analyzed by one-way ANOVA.
Results: Mitochondorial content (P = 0.049) as well as NRF2 (P = 0.002), SIRT-1 (P = 0.007) and Tfam (P = 0.040) levels were significantly lower in obese control than lean group. Mitochondorial content and SIRT-1 levels of three intervention groups of obese+HIIT (P = 0.001), obese+Q10 (P = 0.001) and obese concomitant (P = 0.001) were significantly higher than obese control group and even could precede lean group values (with exception for mitochondorial content in obese+Q10 group (P = 0.001)). Moreover, only in both groups of obese+HIIT ( P= 0.033), and obese concomitant (P = 0.038), NRF2 levels were significantly higher compared to obese control group. However, in none of the intervention groups the Tfam levels had significant differences compared to obese control group (P > 0.05 in all three cases).
Conclusions: Obesity likely suppresses soleus muscle mitochondorial biogenesis, or at least increases the removal rate of pre-existing mitochondria. However, HIIT as well as Q10 supplementation seems to partially capable to restore this down regulation, with a greater effects expected for HIIT. However, more investigations remain to be done due to lack of similar evidence and study limitations.

کلیدواژه‌ها [English]

Obesity
High Intensity Interval Training
Coenzyme Q10
Mitochondoria
Skeletal Muscle

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