اثر تمرین هوازی همراه با مصرف عصارة آبی سیر بر بیان IL-17، IL-22 و مقاومت به انسولین موش‌های صحرایی دیابتی

صادق‌پور فیروزآباد, الناز; عبدی, احمد; عباسی دلویی, آسیه

doi:10.52547/joeppa.16.1.1

اثر تمرین هوازی همراه با مصرف عصارة آبی سیر بر بیان IL-17، IL-22 و مقاومت به انسولین موش‌های صحرایی دیابتی

نوع مقاله : علمی - پژوهشی

نویسندگان

گروه فیزیولوژی ورزشی، واحد آیت‌الله آملی، دانشگاه آزاد اسلامی، آمل، ایران

10.52547/joeppa.16.1.1

چکیده

زمینه و هدف: در حال حاضر شواهد فزاینده‌ای وجود دارد که نشان می‌دهد مسیرهای پیش‌التهابی که توسط سلول‌های T (که IL-17 و IL-22 را ترشح می‌کنند)، تعدیل می‌شوند، نقش مهمی در کنترل سوخت‌وسازی دارند. هدف از پژوهش حاضر بررسی اثر تمرین هوازی همراه با مصرف عصارة آبی سیر بر بیان IL-17، IL-22 و مقاومت‌ به ‌انسولین موش‌های صحرایی دیابتی با استرپتوزوتوسین است.
مواد و روش‌ها: در این تحقیق تجربی،40 سر موش صحرایی نر (سن 5 هفته و وزن 24/15 ± 12/162 گرم) از نژاد ویستار از انستیتو پاستور تهیه و به آزمایشگاه منتقل شدند. پس از انتقال رت‌ها به آزمایشگاه، القای دیابت و آشنایی با فعالیت ورزشی روی نوار گردان ویژة جوندگان، به‌طور تصادفی به پنج گروه کنترل-سالم (CN)، دیابت (DM)، دیابت-تمرین (TDM)، دیابت-سیر (GDM) و دیابت-تمرین-سیر (TGDM) تقسیم شدند. القای دیابت با تزریق داخل‌صفاقی استرپتوزوتوسین به‌صورت تک‌دوز (mg/kg 60) انجام گرفت. قند خون 250 میلی‌گرم بر دسی‌لیتر، حاکی از دیابتی شدن موش‌ها بود. گروه‌های تمرین به مدت هشت هفته برنامة تمرین هوازی (10-18 متر در دقیقه، 10-40 دقیقه در روز، پنج روز در هفته) را روی نوار گردان انجام دادند. روزانه به ازای هر صد گرم وزن موش‌های صحرایی، یک میلی‌لیتر (حدود 4/0 گرم در هر 100 گرم وزن بدن) عصارة سیر گاواژ شد. بیان IL-17 و IL-22 به روش RT-PCR اندازه‌گیری شد. پس از برآورد میزان گلوکز و انسولین ناشتا، از شاخص HOMA-IR برای ارزیابی مقاومت به انسولین استفاده شد. داده‌ها با استفاده از آزمون آنوا و ضریب همبستگی پیرسون در سطح معناداری 05/0 P< بررسی شد.
نتایج: بیان IL-17 (0001/0P=)، IL-22 (0001/0P=) و مقدار HOMA-IR (0001/0P=) در گروه‌های DM نسبت به گروه CN افزایش یافت. IL-17، IL-22 و HOMA-IR در گروه‌های TDM (به‌ترتیب 008/0P=، 040/0P=، 0001/0P=)، GDM (به‌ترتیب 017/0P=، 044/0P=، 0001/0P=) و TGDM (به‌ترتیب 0001/0P=، 0001/0P=، 0001/0P=) نسبت به گروه DM به‌طور معنادار کمتر بود. همچنین Il-17 و IL-22 در گروه TGDM نسبت به گروه TDM (به‌ترتیب 045/0P=، 041/0P=) و GDM (به‌ترتیب 023/0P=، 038/0P=) به‌طور معنادار کمتر بود. بین HOMA-IR با IL-17 (0001/0P=) و IL-22 (001/0P=) همبستگی مثبتی مشاهده شد.
نتیجه‌گیری: یافته‌های پژوهش حاضر نشان می‌دهد که احتمالاً افزایش در میزان بیان نسبی IL-17 و IL-22 ناشی از القای دیابت با افزایش مقاومت به انسولین همراه است. کاهش بیان IL-17 و IL-22 بافت چربی در پی تمرین هوازی و عصارة آبی سیر با کاهش HOMA-IR در موش‌های دیابتی همراه بود. افزون‌بر این، ترکیب تمرین هوازی با عصاره سیر تأثیرات هم‌افزایی داشت و موجب کاهش التهاب و HOMA-IR نسبت به هر کدام به‌تنهایی شد. از آنجا که افراد دیابتی پیوسته در معرض التهاب هستند و با توجه به تغییرات IL-17 و IL-22 در پی فعالیت ورزشی هوازی و مصرف عصاره سیر، استفاده از این دو عامل احتمالاً می‌تواند اهمیت درمانی برای بیماران T2DM داشته باشد.

کلیدواژه‌ها

موضوعات

بیوشیمی و متابولیسم

عنوان مقاله [English]

Effect of Aerobic Training with Aqueous Allium sativum L on IL-17, IL-22 Expression and Insulin Resistance in Diabetic Rats

نویسندگان [English]

Elnaz Sadeghpour Firozabadi
Ahmad Abdi
Asieh Abbassi Daloii

Department of Physical Education and Sport Science, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran

چکیده [English]

Background and Purpose: There is now mounting evidence that pro-inflammatory pathways, which are mediated by T cells (that secrete IL-17 and IL-22), play a critical role in metabolic control. The aim of this study was to examine the effect of aerobic training along with aqueous extract of Garlic (Allium sativum L.) on relative expression of IL-17, IL-22 and insulin resistance in streptozotocin-diabetic rats.
Materials and Methods: In this experimental study, 40 male Wistar rats (age five weeks and weight 162.12 ± 15.24 g) were obtained from the Pasteur Institute and transferred to the laboratory. After transferring the rats to the laboratory, inducing diabetes and familiarizing them with exercise on a rodent treadmill, they were randomly divided into five groups: Control-Normal (CN), Diabetes (DM), Diabetes-Training (TDM), Diabetes-Garlic (GDM) and Diabetes-Training-Garlic (TGDM). Diabetes was induced in the rats by the intraperitoneal administration of a single dose of Streptozotocin (60 mg/kg). Blood glucose of 250 mg/dL was the criterion for diabetic rats. Training groups have performed an aerobic running program (at 10-18 m/min, 10-40 min/day, and five days/week) on a motor-driven treadmill for eight weeks. A dose of one ml of garlic extract /100 g body weight (about 0.4 grams per 100 grams of body weight) was orally administered daily to rats. The expression of IL-17 and IL-22 was measured by RT-PCR method. After estimating fasting glucose and insulin levels, HOMA-IR index was used to evaluate insulin resistance. Data were analyzed using ANOVA and Pearson's correlation coefficient at P < 0.05.

Results: The expression of IL-17 (P = 0.0001), IL-22 (P = 0.0001) and HOMA-IR level (P = 0.0001) increased in DM groups compared to CN group. IL-17, IL-22 and HOMA-IR was significantly lower in TDM (respectively, P = 0.008, P = 0.040, P = 0.0001), GDM (respectively, P = 0.017, P = 0.044, P = 0.0001) and TGDM (respectively, P= 0.0001, P = 0.0001, P = 0.0001) groups than in DM group. Also, IL-17 and IL-22 in the TGDM group lower than TDM (respectively, P = 0.045, P= 0.041) and GDM (respectively, P = 0.023, P = 0.038) groups. A positive correlation was observed between HOMA-IR and IL-17 (P = 0.0001) and IL-22 (P = 0.001).
Conclusions: The findings of the present study show that probably the increase in the relative expression of IL-17 and IL-22 with the induction of diabetes is associated with an increase in insulin resistance. Decreased IL-17 and IL-22 expression adipose tissue followed by aerobic training and aqueous garlic extract were associated with decreased HOMA-IR in diabetic rats. In addition, the combination of aerobic exercise with garlic extract had synergistic effects and reduced inflammation and HOMA-IR compared to each alone. Since diabetics are constantly exposed to inflammation and according to the reduction of IL-17 and IL-22 following aerobic exercise and consumption of garlic extract, the use of these two could possibly be of therapeutic importance for T2DM patients.

کلیدواژه‌ها [English]

Exercise
Garlic
IL-17
IL-22
Insulin Resistance
Diabetes

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