تأثیر هشت هفته مکمل‌دهی اسپیرولینا و تمرین مقاومتی و تناوبی هوازی بر بیان ژن CXCL1 در بافت عضلة اسکلتی و آنزیم‌های کبدی در موش‌های صحرایی نر

نوع مقاله : علمی - پژوهشی

نویسندگان

1 گروه فیزیولوژی ورزش، دانشکدة علوم ورزشی، دانشگاه مازندران، بابلسر، ایران

2 گروه فیزیولوژی، دانشکده دامپزشکی، دانشگاه شیراز، شیراز، ایران

چکیده

زمینه و هدف: کم‌تحرکی و در مقابل آن تمرین جزء عوامل مرتبط با شاخص‌های آسیب کبدی به‌شمار می‌روند. با وجود این هنوز نقش بسیاری از تمرین‌های ورزشی به‌ویژه تمرینات تناوبی و مقاومتی و مصرف برخی مکمل‌های غذایی از جمله اسپیرولینا بر عوامل ایمنی و التهابی کاملاٌ شناخته نشده است؛ بنابراین هدف از این پژوهش تعیین اثر هشت هفته مکمل‌دهی اسپیرولینا و تمرین مقاومتی و تناوبی هوازی بر فعالیت بافت کبد و بیان ژن CXCL1 در بافت عضلة موش‌های صحرایی نر بود.
مواد و روش‌ها: در این پژوهش نیمه‌تجربی، 60 سر موش صحرایی نر نژاد ویستار با سن پنج هفته و میانگین وزن 6/24 ± 4/246 گرم به‌طور تصادفی به شش گروه تمرین تناوبی + مکمل اسپیرولینا، تمرین مقاومتی+ مکمل اسپیرولینا، کنترل+ مکمل اسپیرولینا، تمرین تناوبی+ سالین، تمرین مقاومتی+ سالین، کنترل+ سالین تقسیم‌ شدند. بر این اساس، مقدار مکمل اسپیرولینا در گروه‌های مصرفی مکمل اسپیرولینا 500 میلی‌گرم بر کیلوگرم وزن بدن بود. گروه تمرین تناوبی به مدت هشت هفته و هر هفته پنج جلسه تمرین کردند. همچنین گروه تمرین مقاومتی با بار فزاینده سه جلسه در هفته فعالیت را اجرا کردند. 48 ساعت پس از آخرین جلسة تمرین، حیوانات در گروه‌های مورد آزمایش با ترکیب کتامین زایلازین بی‌هوش شدند و نمونة خون از ورید تحتانی گرفته شد و به‌سرعت بافت عضلة چهارسررانی نمونه‌برداری شد. میزان بیان ژن CXCL1در عضلة چهارسررانی و آنزیم‌های کبدی به‌عنوان شاخص عملکرد کبد در سرم اندازه‌گیری شد. تجزیه‌وتحلیل داده‌های آماری با استفاده از تحلیل واریانس یکسویه انجام گرفت (05/0P£).
نتایج: یافته‌ها نشان داد که پس از هشت هفته تمرین تناوبی و مقاومتی با بار فزاینده، در عضلة چهارسر رانی میزان بیان ژن CXCL1 گروه کنترل بیشتر بود، ولی تفاوت معناداری مشاهده نشد. همچنین، میزان آنزیم‌های کبدی به‌طور معناداری نسبت به گروه کنترل بیشتر بود. همچنین نتایج نشان داد که مصرف اسپیرولینا به‌همراه تمرینات هوازی تناوبی و مقاومتی می‌تواند شاخص‌های عملکرد کبدی را در مقایسه با گروه کنترل به‌طور معناداری تغییر دهد. با این همه بیان ژن CXCL1 در گروه‌های مصرف‌کنندة اسپیرولینا و تمرینات هوازی و مقاومتی در بخشی با گروه کنترل تغییر معناداری نشان نداد.
نتیجه‌گیری: یافته‌های پژوهش حاضر نشان داد که مصرف اسپیرولینا و انجام تمرینات هوازی تناوبی و مقاومتی می‌تواند شاخص‌های عملکردی کبد و بیان ژن CXCL1 در بافت عضلة چهارسررانی را تا حدی تعدیل کند. این نتایج نشان می‌دهد که مصرف اسپیرولینا می‌تواند در بروز پاسخ‌های سازگاری مرتبط با آسیب‌های متابولیکی درگیر باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of eight weeks of spirulina supplementation and resistance and interval aerobic training on skeletal muscle CXCL1 gene expression and liver enzymes in male rats

نویسندگان [English]

  • Mohammad javad Jalali 1
  • Rozita Fathi 1
  • Khadijeh Nasiri 1
  • Abolfazl Akbari 2
1 Department of Exercise Physiology, Faculty of Sports Sciences, Mazandaran University, Babolsar, Iran
2 Department of Physiology, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran
چکیده [English]

Background and Purpose: Inactivity and on the other hand physical exercise are known as factors related to indicators of liver damage. However, the role of many types of interval, including interval and resistance training, and the consumption of certain dietary supplements, including spirulina, on immune and inflammatory factors is not fully understood. Therefore, the aim of this research was to determine the effect of eight weeks of spirulina supplementation and resistance and intermittent aerobic training on liver tissue activity and CXCL1 gene expression in muscle tissue of Males rats.
Materials and Methods: In this semi-experimental research, 65-weeks-old male Wistar rats with an average weight of 246.4 ± 24.6g were randomly assigned to six groups: interval training + spirulina supplement, resistance training + spirulina supplement, control + spirulina supplement, interval training + saline, resistance training + saline, control + saline. Based on this, the amount of spirulina supplement in the spirulina supplement consumption groups was 500 mg/kg body weight. The interval training group performed five sessions per week for eight weeks, and the resistance training group performed three sessions per week with increasing load. 48 hours after the last training session, the animals in the experimental groups were anesthetized with ketamine and xylazine, blood samples were taken from the inferior vein, and quadriceps muscle tissue was quickly removed. CXCL1 gene expression level in quadriceps muscle and liver enzymes were measured as an indicator of liver function in serum. Statistical data were analyzed using one-way analysis of variance (P < 0.05).
Results: The results of this research showed that after 8-weeks of interval and resistance training with increasing load, the expression level of the CXCL1 gene was higher in the quadriceps muscle of the control group, but no significant difference was found. The level of liver enzymes was significantly higher than the control group. The results also showed that spirulina consumption combined with interval aerobic and resistance exercise can significantly alter liver function indicators compared to the control group. However, the expression of the CXCL1 gene in the groups consuming spirulina and aerobic and resistance training did not show a significant change compared to the control group.
Conclusion: the results of this research showed that spirulina and interval and resistance exercises can modulate the functional indicators of the liver and the gene expression of CXCL1 in the quadriceps muscle tissue. These results show that spirulina can be involved in the occurrence of adaptive responses related to metabolic damage.

کلیدواژه‌ها [English]

  • Spirulina Supplementation
  • CXCL1 Gene Expression
  • Resistance Training
  • Interval Training
  • Liver Enzymes

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سابقه مقاله

  • تاریخ دریافت: 11 تیر 1401
  • تاریخ بازنگری: 23 شهریور 1401
  • تاریخ پذیرش: 11 مهر 1401
  • تاریخ اولین انتشار: 02 اسفند 1401
  • تاریخ انتشار: 01 فروردین 1402

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