تأثیر هشت هفته تمرین تناوبی 30-20-10 برسطوح اسپکسین سرم و نیمرخ لیپیدی مردان میانسال چاق

یزدان شناس, حمید; فخرپور, رقیه; قربانیان, بهلول

doi:10.48308/joeppa.2023.103405

تأثیر هشت هفته تمرین تناوبی 30-20-10 برسطوح اسپکسین سرم و نیمرخ لیپیدی مردان میانسال چاق

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه علوم ورزشی، دانشکدة علوم تربیتی و روانشناسی، دانشگاه شهید مدنی آذربایجان، تبریز، ایران

10.48308/joeppa.2023.103405

چکیده

زمینه و هدف: هدف از پژوهش حاضر بررسی تأثیر تمرین تناوبی 30-20-10 بر سطح اسپکسین (spexin) سرم و نیمرخ لیپیدی مردان چاق بود تا بدین‌وسیله، تأثیر فعالیت بدنی بر افزایش اسپکسین و بهبود نیمرخ لیپیدی در مردان چاق سنجیده شود.
مواد و روش‌ها: جامعة آماری پژوهش، 21 مرد چاق (میانگین سنی 06/9± 52/43 سال، وزن 42/16 ± 11/108 کیلوگرم، شاخص تودة بدنی 14/7 ± 42/35 کیلوگرم بر متر مربع) بودند که داوطلبانه در پژوهش حاضر شرکت کردند و به‌طور تصادفی در گروه تمرین (11 نفر) و کنترل (10 نفر) قرار گرفتند. تمرینات به مدت هشت هفته و سه جلسه در هفته با تمرینات 30-20-10 شامل تمرینات تناوبی با شدت‌های تقریبی 30 ثانیه با شدت 30% ، 20 ثانیه با شدت 60 % و10 ثانیه با شدت 90-100% درصد ضربان قلب بیشینه انجام گرفت. این تمرینات یک‌دقیقه‌ای، پنج بار (پنج دقیقه) تکرار شد و پس از پنج بار تکرار، دو دقیقه استراحت کردند و مجدد پنج تکرار بعدی را شروع کردند و در سه تا چهار نوبت دویدن تکرار شد. در پایان مداخله، اسپکسین سرم و نیمرخ لیپیدی به روش الایزا اندازه‌گیری شد. تغییرات درون‌گروهی با استفاده از آزمون t وابسته بررسی شد. در تحلیـل داده‌ها در طرح پیش‌آزمون- پس‌آزمون با گروه کنترل از آزمون آنکوا استفاده شد که در آن نمرات پیش‌آزمون به‌عنوان Covariate به‌کار رفت. تحلیل‌های آماری در سطح آماری 05/0P ≤ انجام گرفت.
نتایج: با بررسی نتایج پژوهش مشخص شد که تمرینات تناوبی 30-20-10 سبب افزایش معنادار میزان اسپکسین در گروه تمرین نسبت به گروه کنترل شد (010/0= P). همچنین تمرینات تناوبی 30-20-10 موجب کاهش معنادار در میزان متغیرهای TG (001/0= P)، LDL-C (001/0P=)، کلسترول (001/0= P) و افزایش معناداری بر HDL-C (001/0= P) شد. بر پایة نتایج مشخص شد، بین کلسترول و تغییرات سطوح سرمی اسپکسین مردان میانسال چاق همبستگی معناداری وجود دارد (004/0= P). همین‌طور بین تغییرات سطوح سرمی اسپکسین و تغییرات برخی از شاخص‌های آنتروپومتریکی مانند شاخص تودة بدنی (05/0= P) و نسبت دور کمر به دور لگن (005/0= P) مردان میانسال چاق همبستگی معناداری مشاهده شد.
نتیجه‌گیری: با توجه به نتایج پژوهش، افزایش معنادار اسپکسین سرم و HDL-Cو کاهش معنادار کلسترول، تری‌گلیسیرید و LDL-C، می‌توان نتیجه گرفت که هشت هفته تمرینات تناوبی 30-20-10، بر سطوح عوامل ذکرشده در مردان میانسال چاق، تأثیرگذار است و شاید سبب افزایش سطوح سرمی اسپکسین و بهبود نیمرخ لیپیدی در این افراد شود.

کلیدواژه‌ها

موضوعات

فیزیولوژی ورزشی (عمومی)

عنوان مقاله [English]

The effect of eight weeks of 30-20-10 interval training on serum spexin levels and lipid profile in obese middle-aged men

نویسندگان [English]

Hamid Yazdanshenas
Roghayeh Fakhrpour
Bahloul Ghorbanian

Department of Sports Sciences, Faculty of Educational Sciences and Psychology, Azerbaijan Shahid Madani University, Tabriz, Iran

چکیده [English]

Background and Purpose: The purpose of this study was to investigate the effect of 30-20-10 interval training on the serum spexin level and lipid profile of obese men in order to measure the effect of physical activity on increasing spexin and improving lipid profile in obese men.
Materials and Methods: Statistical population of Yezhohesh, 21 obese men (mean age 43.52±9.06 years, weight 108.11±16.42 kg, body mass index 35.42±7.14 kg/m²) who voluntary participated in the present study and were randomly divided into training (11 people) and control (10 people) groups. Training were performed for eight weeks and three sessions per week with the 30-20-10 exercise protocol, including interval training with approximately intensities of 30 seconds with 30%, 20 seconds with 60% and 10 seconds with 90-100% of maximum heart rate. These one-minute training were repeated five times (five minutes) and after 5 repetitions, they rested for two minutes and started the next five repetitions again, and it was repeated in three-four times of running. At the end of the intervention, serum spexin and lipid profile were measured by ELISA method. Within-group changes were analyzed using the dependent t-test. ANCOVA test was used in the data analysis in the pre-test-post-test design with the control group. where pre-test scores were used as covariate. Statistical analyzes were performed at the statistical level of P ≤ 0.05.
Results: By examining the research results, it was found that 10-20-30 interval training caused a significant increase in the amount of spexin in the training group compared to the control group (P=0.010). Also, interval training 10-20-30 causes a significant decrease in the amount of TG variables (P=0.001), LDL-C (P=0.001), cholesterol (P=0.001) and a significant increase in HDL-C. (P=0.001) has been Based on the results, it was found that there is a significant relationship between cholesterol and changes in the serum levels of spexin in obese middle-aged men (P=0.004). Also, there is a significant relationship between changes in serum levels of spexin and changes in some anthropometric indices such as body mass index (P=0.05) and ratio of waist circumference to hip circumference (P=0.005) of obese middle-aged men.
Conclusion: According to the results of the research, a significant increase in serum spexin and HDL-C and a significant decrease in cholesterol, triglyceride and LDL-C, it can be concluded that eight weeks of 30-20-10 interval training has an effect on the levels of the factors mentioned in obese middle-aged men. It can probably increase the serum levels of spexin and improve the lipid profile in these people.

کلیدواژه‌ها [English]

Triglyceride
Cholesterol
High-density lipoprotein
Low-density lipoprotein

مراجع

1.Ceylan Hİ, Saygın Ö, Özel Türkçü Ü. Assessment of acute aerobic exercise in the morning versus evening on asprosin, spexin, lipocalin-2, and insulin level in overweight/obese versus normal weight adult men. Chronobiology International. 2020;37(8):1252-68.

2.Daviglus ML, Talavera GA, Avilés-Santa ML, Allison M, Cai J, Criqui MH, et al. Prevalence of major cardiovascular risk factors and cardiovascular diseases among Hispanic/Latino individuals of diverse backgrounds in the United States. Jama. 2012;308(17):1775-84.

3.Habibian M, Amirnia Shobi S, Zakeri Khatir E. The Effects of 8 weeks of regular aerobic exercise on the TNF-α levels and lipid profile in obese girls. Journal of Sabzevar University of Medical Sciences. 2021;28(1):134-42.) In Persian(

4.Baghersalimi M, Fathi R, Kazemi S. The Effect of Aerobic Training on Lipid Accumulation Product, Visceral Adiposity, Triglyceride-Glucose and Mcauley Indices in Early Pubertal Obese/Overweight Girls. Sport Physiology. 2020;12(46):95-116. ) In Persian(

5.Mirabeau O, Perlas E, Severini C, Audero E, Gascuel O, Possenti R, et al. Identification of novel peptide hormones in the human proteome by hidden Markov model screening. Genome research. 2007;17(3):320-7.

6.Lin C-y, Huang T, Zhao L, Zhong LL, Lam WC, Fan B-m, et al. Circulating spexin levels negatively correlate with age, BMI, fasting glucose, and triglycerides in healthy adult women. Journal of the Endocrine Society. 2018;2(5):409-19.

7.Bacopoulou F, Apostolaki D, Mantzou A, Doulgeraki A, Pałasz A, Tsimaris P, et al. Serum Spexin is correlated with lipoprotein (a) and androgens in female adolescents. Journal of Clinical Medicine. 2019;8(12):2103.

8.Fang P, Ge R, She Y, Zhao J, Yan J, Yu X, et al. Adipose tissue spexin in physical exercise and age-associated diseases. Ageing Research Reviews. 2022;73:101509.

9.Khadir A, Kavalakatt S, Madhu D, Devarajan S, Abubaker J, Al-Mulla F, et al. Spexin as an indicator of beneficial effects of exercise in human obesity and diabetes. Scientific Reports. 2020;10(1):1-11.

10.Al-Daghri NM, Al-Hazmi HA, Al-Ajlan A, Masoud MS, Al-Amro A, Al-Ghamdi A, et al. Associations of spexin and cardiometabolic parameters among women with and without gestational diabetes mellitus. Saudi Journal of Biological Sciences. 2018;25(4):710-4.

11.Gu L, Ma Y, Gu M, Zhang Y, Yan S, Li N, et al. Spexin peptide is expressed in human endocrine and epithelial tissues and reduced after glucose load in type 2 diabetes. Peptides. 2015;71:232-9.

12.Gunnarsson TP, Bangsbo J. The 10-20-30 training concept improves performance and health profile in moderately trained runners. Journal of Applied Physiology. 2012.

13.Gliemann L, Gunnarsson TP, Hellsten Y, Bangsbo J. 10‐20‐30 training increases performance and lowers blood pressure and VEGF in runners. Scandinavian journal of medicine & science in sports. 2015;25(5):e479-e89.

14.Andrade PA, Silveira BS, Rodrigues AC, Da Silva FO, Rosa CB, Alfenas RG. Effect of exercise on concentrations of irisin in overweight individuals: a systematic review. Science & Sports. 2018;33(2):80-9.

15.Fang P, Zhang L, Yu M, Sheng Z, Shi M, Zhu Y, et al. Activiated galanin receptor 2 attenuates insulin resistance in skeletal muscle of obese mice. Peptides. 2018;99:92-8.

16.Zahir IM, Ogawa S, Dominic NA, Soga T, Parhar IS. Spexin and Galanin in Metabolic Functions and Social Behaviors With a Focus on Non-Mammalian Vertebrates. Frontiers in Endocrinology. 2022;13.

17.Ma A, Bai J, He M, Wong AO. Spexin as a neuroendocrine signal with emerging functions. General and comparative endocrinology. 2018;265:90-6.

18.Leciejewska N, Pruszyńska-Oszmałek E, Mielnik K, Głowacki M, Lehmann TP, Sassek M, et al. Spexin Promotes the Proliferation and Differentiation of C2C12 Cells In Vitro—The Effect of Exercise on SPX and SPX Receptor Expression in Skeletal Muscle In Vivo. Genes. 2021;13(1):81.

19.Tran A, Loganathan N, McIlwraith EK, Belsham DD. Palmitate and nitric oxide regulate the expression of spexin and galanin receptors 2 and 3 in hypothalamic neurons. Neuroscience. 2020;447:41-52.

20.Kolodziejski PA, Pruszynska-Oszmalek E, Micker M, Skrzypski M, Wojciechowicz T, Szwarckopf P, et al. Spexin: A novel regulator of adipogenesis and fat tissue metabolism. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids. 2018;1863(10):1228-36.

21.Yavuzkir S, Ugur K, Deniz R, Ustebay DU, Mirzaoglu M, Yardim M, et al. Maternal and umbilical cord blood subfatin and spexin levels in patients with gestational diabetes mellitus. Peptides. 2020;126:170277.

22.Gharari Arefi R, Hemati Nafar M, Kordi MR. The effect of a high intensity interval training program on lipid profile in sedentary young men. Journal of sport Biosciences. 2014;6(3):259-72. )In Persian(

23.Malekinezhad H, Moflehi D, Abbasi H, Behzadi A. Effect of low or high volume of high-intensity interval training protocols on the leptin and lipid profile in men with type 2 diabetes. Journal of Community Health Research. 2019;8(4):228-36. )In Persian(

24.Lakka TA, Lakka H-M, Rankinen T, Leon AS, Rao D, Skinner JS, et al. Effect of exercise training on plasma levels of C-reactive protein in healthy adults: the HERITAGE Family Study. European heart journal. 2005;26(19):2018-25.