اثر تمرین تناوبی سرعتی بر تعادل اکسایشی- ضداکسایشی بیضه و شاخص‌های تولید مثلی موش‌های آزمایشگاهی بزرگ

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، دانشگاه شاهد. تهران، ایران

چکیده

زمینه و هدف: در جوامع امروزی افراد زیادی از اختلال عملکرد دستگاه تناسلی و ناباروری رنج می‌برند. عوامل گوناگونی مانند کم‌تحرکی، برخی بیماری‌ها و مصرف داروها می‌توانند در بروز اختلال باروری نقش داشته باشند و سبب کاهش توان تولید مثلی شوند. از سازوکارهای اصلی اختلال تولید مثل تولید بنیان‌های آزاد و افزایش فشار اکسایشی در بافت بیضه است. تمرین تناوبی سرعتی (SIT) از کارامدترین انواع تمرینات ورزشی از نظر زمانی نسبت به سایر شیوه‌های فعالیت ورزشی است که برای ارتقای میزان فعالیت بدنی و ورزشی می‌تواند به‌کار رود. هدف از پژوهش حاضر، بررسی تأثیر SIT بر وضعیت اکسایشی- ضداکسایشی بیضه و شاخص‌های عملکرد تولید مثلی موش‌های آزمایشگاهی بزرگ بود.
مواد و روش‌ها: در این پژوهش تجربی 16 سر موش بالغ نر آزمایشگاهی بزرگ نژاد ویستار به‌طور تصادفی به گروه‌های کنترل و فعالیت ورزشی تقسیم شدند. سپس گروه فعالیت ورزشی به مدت هشت هفته، هفته‌ای سه جلسه تمرین کردند. 48 ساعت پس از آخرین جلسة تمرین، بیضة موش‌ها پس از بی‌هوشی برداشته و به آزمایشگاه منتقل شد و فعالیت آنزیم‌های سوپراکسید دیسموتاز (SOD) و گلوتاتیون پراکسیداز (GPX)، ظرفیت تام ضداکسایشی (TAC) و مقادیر مالون‌دی‌آلدهید (MDA) و همچنین شاخص‌های عملکرد تولید مثلی مهم در بافت بیضه شامل شاخص تمایز لوله‌ای (TDI)، شاخص اسپرمیوژنز (SI) و ضریب تجمعی (RI) تعیین شد. داده‌ها با استفاده از آزمون تی مستقل در سطح آلفای 05/0 تجزیه‌وتحلیل شد.
نتایج: تحلیل داده‌ها نشان داد میزان MDA بافت بیضة گروه تمرین نسبت به گروه کنترل در حد معناداری بیشتر بود (001/0P˂). بین دو گروه از نظر فعالیت SOD و مقادیر TAC بافت بیضه تفاوت معناداری وجود نداشت؛ ولی میزان فعالیت GPX در گروه تمرین در حد معناداری بیشتر از گروه کنترل بود (05/0P˂). همچنین تعداد سلول‌های لیدیگ، TDI و RI در گروه تمرین در حد معناداری نسبت به گروه کنترل کمتر بود (05/0P˂)؛ ولی از نظر SI بین دو گروه تفاوت معناداری مشاهده نشد (05/0˃P).
نتیجه‌گیری: تمرین تناوبی سرعتی ممکن است فشار اکسایشی را در بافت بیضه افزایش دهد و با تأثیر منفی بر شاخص‌های مهم اسپرماتوژنیک در بیضه، سبب ایجاد اختلال در عملکرد تولید مثلی شود. انجام پژوهش‌های بیشتر در این زمینه با کنترل عوامل اثرگذار و متغیرهای تمرینی (شدت، مدت و حجم تمرین) توصیه می‌شود. 

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of sprint interval training on testis oxidant/antioxidant status and reproductive indices of adult wistar rats

نویسندگان [English]

  • Mahmood Tajik Khaveh
  • Mohammad Rahmani
  • Ali Samadi
Department of Physical Education and Sports Sciences, Faculty of Humanities, Shahed University. Tehran Iran
چکیده [English]

Background and Purpose: In contemporary modern societies a lot of people suffer from reproductive system dysfunction and infertility. Various factors such as sedentariness, some diseases and medicines play a role in incidence of reproductive dysfunction, which can lead to decreased reproductive capacity. One of the main mechanisms of which is the increased free radical’s production and oxidative stress in testis. Sprint interval training (SIT) is one of the most time-efficient types of exercise comparing to other exercise methods, which could be used to promote physical activity level. The aim of the present study was to determine the effect of SIT on testis oxidant-antioxidant status and some reproductive indices in adult Wistar rats.
Materials and Methods: In this experimental study, 16 adult male Wistar rats wereobtained and randomly divided into: 1) control and 2) training groups. Then, the training group practiced 3 times a week for eight weeks. Forty-eight hours after the last exercise session, the rats' testis were removed after anesthesia and transferred to the laboratory and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPX), as well as total antioxidant capacity (TAC) and malondialdehyde (MDA) levels were assessed. Moreover, main reproductive function indices including tubular differentiation index (TDI), spermiogenesis index (SI), and cumulative index (RI) were determined. Data were analyzed using independent samples t-test at the alpha = 0.05.
 
Results: The statistical analysis revealed that the MDA level of the testis in the training group was significantly higher than the control group (P<0.001). There was no significant difference between groups in terms of the SOD activity and TAC. However, in the training group the GPX activity level was significantly higher comparing to the control group (P<0.05). Moreover, the number of Leydig cells, TDI, and RI in the training group were significantly lower in comparison with the control group (P˂0.05); Nevertheless, no significant difference was observed between groups in terms of SI (P>0.05).
Conclusion: SIT may increase oxidative stress in the testicular tissue and negatively affect the main spermatogenic indices in testis, which can lead to reproductive dysfunction. Conducting more research in this area while controlling the other influential factors and training variables (intensity, duration, and volume) is recommended.
 

کلیدواژه‌ها [English]

  • High-intensity interval training
  • Oxidative stress
  • Spermatogenesis

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سابقه مقاله

  • تاریخ دریافت: 28 آذر 1401
  • تاریخ بازنگری: 11 بهمن 1401
  • تاریخ پذیرش: 18 بهمن 1402
  • تاریخ اولین انتشار: 18 بهمن 1402
  • تاریخ انتشار: 01 تیر 1402

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