تاثیر شدت‌های مختلف تمرین مقاومتی بر بیان microRNA-204 و فاکتور نسخه‌برداری استئوژنز Runx2 و برخی خواص بیومکانیکی استخوان موش‌های صحرایی نر سالمند نژاد ویستار

همتی فارسانی, زهرا; بنی طالبی, ابراهیم; فرامرزی, محمد; بی غم صادق, امین

doi:10.52547/joeppa.14.1.38

تاثیر شدت‌های مختلف تمرین مقاومتی بر بیان microRNA-204 و فاکتور نسخه‌برداری استئوژنز Runx2 و برخی خواص بیومکانیکی استخوان موش‌های صحرایی نر سالمند نژاد ویستار

نوع مقاله : علمی - پژوهشی

نویسندگان

¹ گروه تربیت بدنی و علوم ورزشی، دانشگاه اردکان، یزد، ایران

² گروه علوم ورزشی، دانشکدة ادبیات و علوم انسانی، دانشگاه شهرکرد، شهرکرد، ایران

³ گروه فیزیولوژی ورزشی ، دانشکده علوم ورزشی، دانشگاه اصفهان، اصفهان، ایران

⁴ دانشکدة دامپزشکی، دانشگاه شهرکرد، شهرکرد، ایران

10.52547/joeppa.14.1.38

چکیده

هدف: استخوان‌ها به‌طور وسیعی تحت تأثیر تغذیه، سطح فعالیت و سبک زندگی قرار می‌گیرند و سازگاری‌های ساختاری در شکل و اندازة استخوان از طریق نیروهای ناشی از تحمل وزن و نیروهای اعمال‌شده توسط عضلات اتفاق می‌افتد. ازاین‌رو هدف از مطالعة حاضر این است که آیا شدت‌های مختلف تمرین مقاومتی بر بیان mir-204 و برخی خواص بیومکانیکی استخوان موش‌های نر سالمند نژاد ویستار تأثیر دارد؟
روش‌ها: 24 سر موش نر سالمند نژاد ویستار (میانگین سن 23 ماه، میانگین وزن 93/437گرم) در سه گروه مساوی هشت‌تایی شامل تمرینات مقاومتی با شدت متوسط (60 درصد حداکثر ظرفیت حمل ارادی (MVCC)) و تمرینات مقاومتی با شدت بالا (80 درصد MVCC) و گروه کنترل قرار گرفتند و به‌طور تصادفی براساس وزن اولیه تقسیم شدند. تمرینات مقاومتی با استفاده از نردبان مقاومتی و 5 روز در هفته به مدت 8 هفته انجام گرفت. بعد از پایان دورة تمرین بیانmir-204 در بافت مغز استخوان درشت‌نئی به روش RT–PCR اندازه‌گیری و با استفاده از فرمول ^ΔΔCT- 2 کمی شدند. همچنین از آزمون خمش سه‌نقطه‌ای برای تعیین خواص بیومکانیکی استخوان استفاده شد. تحلیل آماری با استفاده از تحلیل واریانس تک‌راهه و آزمون کروسکال والیس با سطح 05/ 0P≤ انجام گرفت.
نتایج: میزان بیان mir-204(539/0P=)، Runx2 (960/0P=)، مدولاسیون (82/0P=)، قدرت بیشینه و استرس (80/0P=)، انرژی شکست (99/0P=) و نیروی استخوان (81/0P=) در گروه‌های تمرینی با شدت بالا و متوسط نسبت به گروه کنترل تفاوت معناداری نداشت.
نتیجه‌گیری: با توجه به عدم معناداری در نتایج تحقیق، به‌نظر می‌رسد مدت تمرین برای تأثیرگذاری بر متغیرهای استخوان کافی نبوده است، ازاین‌رو نیاز است دوره‌های طولانی‌تر این نوع تمرینات ورزشی، در تحقیقات آینده بررسی شود.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of different intensities Resistance Training on expression mir-204 and transcription factors of osteogenic, Runx2 and biomechanical properties on bone in Old male Wistar rats

نویسندگان [English]

ZAHRA HEMATI FARSANI ¹
Ebrahim Banitalebi ²
Mohamad Faramarzi ³
Amin Bigham-Sadegh ⁴

¹ Department of Physical Education and Sports Science, Ardakan University, Yazd, Iran

² Department of Sports Sciences, Faculty of Literature and Humanities, Shahrekord University, Shahrekord, Iran

³ Department of exercise physiology, University of Isfahan, Isfahan, Iran,

⁴ , Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran

چکیده [English]

Purpose: Bones are largely influenced by nutrition, activity levels and lifestyle, and structural adaptations in the form and size of the bone occur by the forces of weight bearing and forces applied by the muscles. Therefore, the aim of the present study was to determine whether different resistance training intensities affect on expression of mir-204 and transcription factors of osteogenic, Runx2 and biomechanical properties on bone in old male wistar rats.
Methods: This experimental study was done on 24 Wistar male rats (23 months old and with an average weight of 437.93 gram). They were randomly divided into three equal groups (n=8) include moderate (60% maximum voluntary carrying capacity: MVCC) and high intensity (80% MVCC) resistance training and control according to initial weight. The two training groups completed eight weeks of training program, five days a week according to resistance protocols. After completing training, expression of mir-204 on bone marrow were measured RT-PCR and Three – point bending test was used to determine bone biomechanical properties. The statistical analysis was performed using Kruskal-Wallis and one-way ANOVA test with significance level of P < 0.05
Results: There was no significance in expression of mir-204 (P = 0.539), Runx2 (P = 0.960), module (P = 0.82), stress (P = 0.80), fracture energy (P = 0.99) and bone force (P = 0.81) between the intervention groups and control group.
Conclusion: It seems that considering the lack of meaning in the results of this study, it seems that the duration of exercise was not sufficient to influence bone variables. then longer periods of this type of exercise exercise will be investigated in future research.
Keywords: Biomechanical properties, Resistance training, Mir-204, Runx2

کلیدواژه‌ها [English]

bone metabolism
biomechanical properties
resistance training
mir
204
Runx2

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