The effect of different intensities Resistance Training on expression mir-204 and transcription factors of osteogenic, Runx2 and biomechanical properties on bone in Old male Wistar rats

Document Type : original article


1 Department of Physical Education and Sports Science, Ardakan University, Yazd, Iran

2 Department of Sports Sciences, Faculty of Literature and Humanities, Shahrekord University, Shahrekord, Iran

3 Department of exercise physiology, University of Isfahan, Isfahan, Iran,

4 , Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran


Purpose:  Bones are largely influenced by nutrition, activity levels and lifestyle, and structural adaptations in the form and size of the bone occur by the forces of weight bearing and forces applied by the muscles. Therefore, the aim of the present study was to determine whether different resistance training intensities affect on expression of mir-204 and transcription factors of osteogenic, Runx2 and biomechanical properties on bone in old male wistar rats.  
Methods: This experimental study was done on 24 Wistar male rats (23 months old and with an average weight of 437.93 gram). They were randomly divided into three equal groups (n=8) include moderate (60% maximum voluntary carrying capacity: MVCC) and high intensity (80% MVCC) resistance training and control according to initial weight. The two training groups completed eight weeks of training program, five days a week according to resistance protocols. After completing training, expression of mir-204 on bone marrow were measured RT-PCR and Three – point bending test was used to determine bone biomechanical properties. The statistical analysis was performed using Kruskal-Wallis and one-way ANOVA test with significance level of P < 0.05
Results: There was no significance in expression of mir-204 (P = 0.539), Runx2 (P = 0.960), module (P = 0.82), stress (P = 0.80), fracture energy (P = 0.99) and bone force (P = 0.81) between the intervention groups and control group.
Conclusion: It seems that considering the lack of meaning in the results of this study, it seems that the duration of exercise was not sufficient to influence bone variables. then longer periods of this type of exercise exercise will be investigated in future research.
Keywords: Biomechanical properties, Resistance training, Mir-204, Runx2


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  • Receive Date: 09 December 2018
  • Revise Date: 07 April 2021
  • Accept Date: 21 April 2021
  • First Publish Date: 22 June 2021
  • Publish Date: 22 June 2021