Comparison of the effect of TRX and traditional resistance training on serum levels of some liver enzymes in inactive women

Document Type : original article


Department of Sports Science, Faculty of Literature and Humanities, University of Qom, Qom, Iran


Purpose: The liver is one of the most sensitive tissues targeted by exercise-induced oxidative stress, and hepatic aminotransferases are critical indicators for determining liver cell damage; Therefore, the present study aimed to compare the effect of eight weeks of TRX and traditional resistance training on some liver enzymes (aspartate aminotransferase and alanine aminotransferase) in inactive women.
Methods: In this quasi-experimental study, 28 inactive girls with a mean age of 21.07 ± 1.41 years and a body mass index of 22.52 ± 4.25 kg/m2 were randomly divided into three groups: TRX training, traditional resistance training and control. Experimental groups conducted the traditional resistive training and TRX three sessions a week for eight weeks, While the control group did not participate in any training program during the study. Blood samples were taken from all subjects before starting protocol and 48 hours after the last training session and were used to analyze Aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Data were analyzed using Kolmogorov-Smirnov test, dependent t-test and one-way analysis of variance, and the significance level was considered P < 0.05.
Results: The results showed that there was no significant difference in serum levels of AST and ALT enzymes from pre to posttest in TRX, traditional resistance and control groups (P > 0.05). Also, no difference between groups was observed in any of the factors (P > 0.05).
Conclusion: The results of the present study showed that there was no significant difference between TRX training and traditional resistance to serum levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in inactive women. Therefore, the effects of TRX and traditional resistance training on liver enzyme indices were the same and no difference was observed between these two types of training methods.


  1. Slentz CA, Bateman LA, Willis LH, Shields AT, Tanner CJ, Piner LW, et al. Effects of aerobic vs. resistance training on visceral and liver fat stores, liver enzymes, and insulin resistance by HOMA in overweight adults from STRRIDE AT/RT. American Journal of Physiology-Endocrinology and Metabolism. 2011;301(5):E1033-E9.
  2. Wilmor JH, Castiel DL. Physiology of Exercise and Physical Activity. Translated by Zia Moeini and Farhad Rahmaniniya and Hamid Rajabi and Hamid Agha ali Nejad and Fatemeh Salami. 2004:93-103.
  3. Clarkson PM, Kearns AK, Rouzier P, Rubin R, Thompson PD. Serum creatine kinase levels and renal function measures in exertional muscle damage. Medicine and science in sports and exercise. 2006;38(4):623.
  4. Washington IM, Van Hoosier G. Clinical biochemistry and hematology. The laboratory rabbit, guinea pig, hamster, and other rodents: Elsevier; 2012. p. 57-116.
  5. 5. Cavas L, Tarhan L. Effects of vitamin-mineral supplementation on cardiac marker and radical scavenging enzymes, and MDA levels in young swimmers. International journal of sport nutrition and exercise metabolism. 2004;14(2):133-46.
  6. Nia FR, Farzaneh E, Damirchi A, Majlan AS. Effect of L-glutamine supplementation on electromyographic activity of the quadriceps muscle injured by eccentric exercise. Iranian journal of basic medical sciences. 2013;16(6):808.
  7. Darvishi-Khezri H, Karami H, Naderisorki M, Zahedi M, Razavi A, Kosaryan M, et al. Moderate to severe liver siderosis and raised AST are independent risk factors for vitamin D insufficiency in β-thalassemia patients. Scientific Reports. 2020;10(1):1-8.
  8. Li L, Wei Y, Fang C, Liu S, Zhou F, Zhao G, et al. Exercise retards ongoing adipose tissue fibrosis in diet-induced obese mice. Endocrine connections. 2021;10(3):325-35.
  9. Kawanishi N, Yano H, Mizokami T, Takahashi M, Oyanagi E, Suzuki K. Exercise training attenuates hepatic inflammation, fibrosis and macrophage infiltration during diet induced-obesity in mice. Brain, behavior, and immunity. 2012;26(6):931-41.
  10. Ostojic SM. Characteristics of elite and non-elite Yugoslav soccer players: correlates of success. Journal of sports science and medicine. 2003;2(1):34-5.
  11. Bompa T, Buzzichelli C. Periodization training for sports, 3e: Human kinetics; 2015.
  12. Tan B. Manipulating resistance training program variables to optimize maximum strength in men: a review. The Journal of Strength & Conditioning Research. 1999;13(3):289-304.
  13. Zhengying TZX. Effects of Overtraining on Activities of Myocardial CK, AST and their Isozyme, SOD and LDH in Rats. JOURNAL OF BEIJING UNIVERSITY OF PHYSICAL EDVCATION. 1999.
  14. Kim JV, Wu GY. Body Building and Aminotransferase Elevations: A Review. Journal of Clinical and Translational Hepatology. 2020;8(2):161.
  15. Khajehlandi M, Janbozorgi M. Effect of one session of resistance training with and without blood flow restriction on serum levels of creatine kinase and lactate dehydrogenase in female athletes. Journal of Clinical and Basic Research. 2018;2(2):5-10.
  16. Shamsoddini A, Sobhani V, Chehreh MEG, Alavian SM, Zaree A. Effect of aerobic and resistance exercise training on liver enzymes and hepatic fat in Iranian men with nonalcoholic fatty liver disease. Hepatitis monthly. 2015;15(10).
  17. Behm DG, Drinkwater EJ, Willardson JM, Cowley PM. The use of instability to train the core musculature. Applied physiology, nutrition, and metabolism. 2010;35(1):91-108.
  18. Beim GM, Giraldo JL, Pincivero DM, Borror MJ, Fu FH. Abdominal strengthening exercises: a comparative EMG study. Journal of Sport Rehabilitation. 1997;6(1):11-20.
  19. McGill SM, Cannon J, Andersen JT. Analysis of pushing exercises: Muscle activity and spine load while contrasting techniques on stable surfaces with a labile suspension strap training system. The Journal of Strength & Conditioning Research. 2014;28(1):105-16.
  20. Fong SS, Tam Y, Macfarlane DJ, Ng SS, Bae Y-H, Chan EW, et al. Core muscle activity during TRX suspension exercises with and without kinesiology taping in adults with chronic low back pain: implications for rehabilitation. Evidence-based complementary and alternative medicine. 2015;2015.
  21. Yu K-H, Suk M-H, Kang S-W, Shin Y-A. Effects of combined resistance training with TRX on physical fitness and competition times in fin swimmers. International Journal of Sport Studies. 2015;5(5):508-15.
  22. Plowman SA, Smith DL. Exercise physiology for health fitness and performance: Lippincott Williams & Wilkins; 2013.
  23. Sadeghi S, Asad Mr, Ferdosi Mh. The effect of twelve weeks endurance training on liver enzymes levels in Iranian obese women. Research in Sport Medicine and Technology Research in Sport Medicine and Technology. 2017;15(13):49-60.
  24. Ranjbar R, Hasanvand H, Habibi AH, Goharpey S. Comparison of the Effect of TRX and Traditional Resistance Training on Some Factors of Body Composition and Balance in Sedentary Men. Jundishapur Scientific Medical Journal. 2018;16(6):621-30.
  25. Heltne T, Welles C, Riedl J, Anderson H, Howard A. Effects of TRX versus Traditional Training Programs on Core Endurance and Muscular Strength. 2013.
  26. Lamprecht M, Moussalli H, Ledinski G, Leschnik B, Schlagenhauf A, Koestenberger M, et al. Effects of a single bout of walking exercise on blood coagulation parameters in obese women. Journal of applied physiology. 2013;115(1):57-63.
  27. Mougios V. Exercise biochemistry: Human Kinetics Publishers; 2019.
  28. Moosavi-Sohroforouzani A, Ganbarzadeh M. Reviewing the physiological effects of aerobic and resistance training on insulin resistance and some biomarkers in non-alcoholic fatty liver disease. KAUMS Journal (FEYZ). 2016;20(3):282-96.
  29. Mansoori Z, MS,  FD,  Nk,  VH, Haghdel A. The effect of green tea extract on indices of liver damage (ALT and AST) caused by high intensity interval training in professional soccer players. Sport and Exercise Physiology. 2018;1(11):97-106.
  • Receive Date: 20 September 2020
  • Revise Date: 08 June 2021
  • Accept Date: 18 June 2021
  • First Publish Date: 21 November 2021
  • Publish Date: 21 April 2022