The effect of six weeks’ progressive resistance training on hippocampus BDNF gene expression and serum changes of TNF-α in diabetic wistar rats

Document Type : original article


1 Sport Science Department, Human Faculty, Semnan University, Semnan, Iran

2 Sport Physiology Department, Physical Education and Sport Sciences Faculty, Kharazmi University, Tehran, Iran


Purpose: Exercise is a powerful driver for reducing the complications of diabetes. The aim of the present study was to investigate the effect of progressive resistance training on the expression of brain-derived neurotrophic factor gene in the hippocampus and serum changes of TNF α in Wistar diabetic rats.
Methods: For this study, 36 rats weighing 160 ± 10 g were randomly divided into three groups of 12 diabetic (D), diabetic and progressive resistance training (DRT) and control (C) groups. STZ solution (50 mg / kg) was used to induce diabetes. Progressive resistance training protocol three days a week, each session consisting of four to nine sets of 110cm vertical ladders with 2cm stairs and 85 ° angles of 50%, 75%, 90% with 100% maximum load capacity. Animals were dissected 48 hours after training. Samples were extracted 48 hours after GTT test. The animals were dissected and transferred to the laboratory of the Endocrine and Metabolism Research Institute to measure BDNF gene expression. BDNF gene expression was evaluated using Real Time PCR technique.
Results: Results showed that resistance training increased (P = 0/001) BDNF gene expression and decreased (P = 0/002) TNF α expression. Hippocampal weight gain was also associated with increased BDNF gene expression.
Conclusion: resistance training can prevent hippocampal tissue analysis due to diabetes and is important in diabetic patients because of the positive role of the hippocampus on memory. TNF α expression was also decreased. Therefore, it is recommended to improve the physical health of diabetic patients.


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  • Receive Date: 17 August 2020
  • Revise Date: 01 June 2021
  • Accept Date: 18 June 2021
  • First Publish Date: 30 November 2021
  • Publish Date: 21 March 2022