The effect of two-month concurrent training and caloric restriction on P65 amount in Peripheral blood mononuclear cells of inactive middle-aged men

Document Type : original article


1 Faculty of Sports and Health Sciences, Shahid Beheshti University, Tehran, Iran

2 Faculty of Physical Education and Sports Sciences, University of Tabriz, Tabriz, Iran

3 Tabriz University of Medical Sciences, Tabriz, Iran

4 Payame Noor University, Tehran, Iran


 Purpose: Recognition of mechanisms involved in inflammatory disorders through the aging process and providing appropriate preventive or modulator strategies to improve quality of life are always important challenges in the field of life sciences. Therefore, the present study was conducted to determine the effect of two-month concurrent training (RT+HIIT) and caloric restriction on serum CRP and P65 amount in Peripheral blood mononuclear cells of inactive middle-aged men.
Methods: Twenty-four inactive, healthy men participated in three homogeneous groups: Caloric restriction (CR), Concurrent training (T) and Concurrent training with Caloric restriction (CRT) groups. Calorie restriction and energy cost in five days a week was about six kcal·kg-1·day-1. The concurrent training was included five days per week (two sessions of resistance training with 75 to 80% 1RM and three sessions of high intensity interval training with 80-85% heart rate reserve). Baseline and post-intervention p65 in the total and phosphorylated (p-p65) levels in PBMCs were assessed by ELISA. Serum CRP concentration was also measured using immunoturbidimetric.
Results: Weight loss in CRT group was significantly higher than the other two groups (P < 0.05). However, the changes range in fat percentage, maximal oxygen consumption and one-repetition maximum (1RM) of T and CRT groups were significantly higher than CR group (P < 0.05). Changes in phosphorylated P65 and p-p65 / total p65 ratio in PBMCs of T and CRT groups were significantlyhigher than CR group (P < 0.05).
Conclusion: Based on the present results, two months of concurrent training and caloric restriction can reduce some of inflammatory markers in inactive men. In addition, the effect of concurrent training with and without calorie restriction on the initiators of inflammatory factors is greater than the calorie restriction intervention alone. Therefore, concurrent training and caloric restriction are recommended for prevention and confronting obesity and its inflammatory consequences, especially in middle age.


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  • Receive Date: 11 October 2019
  • Revise Date: 11 June 2020
  • Accept Date: 22 June 2020
  • First Publish Date: 23 September 2021
  • Publish Date: 23 September 2021