Purpose: Diabetes is a metabolic disorder characterized by hyperglycemia and plays an important role in the development of cardiac apoptosis. Evidence suggests that exercise can affect some of the signaling pathways associated with apoptosis. Evidence suggests that exercise can affect some of the apoptosis-related signaling pathways. The aim of this study was to compare the effect of aerobic and resistance training on insulin resistance index and BCL-2 to BAX ratio in male Wistar diabetic rats. Methods: In this experimental study, 25 male Wistar rats in the weight range of 200 to 250 g were randomly divided into five groups: Aerobic training (n = 6), Resistance training (n = 6), Sham (n = 6), Control (n = 4), Healthy (n = 3) Rats became diabetic by consuming a high-fat diet for six weeks and after six weeks with a single dose of 30 mg / kg streptozotocin injection. Rats in the aerobic group trained on a treadmill for four weeks and five sessions per week, and rats in the resistance group trained on a ladder for four weeks and six sessions per week. Results: The results showed that there was a significant difference between the aerobic and resistance groups of BAX protein and the amount of this protein in the resistance group was lower than aerobic (P= 0.014). There was a significant difference between BCL-2 and BAX ratio between aerobic and resistance groups and this ratio was higher in the resistance group than the aerobic group (P = 0.05). Also, the rate of insulin resistance index in the aerobic group (P = 0.005) and in the resistance group (P = 0.004) after exercise decreased more than before exercise and this difference was significant. Conclusion: It seems that both resistance training and aerobic training are effective in reducing the amount of insulin resistance index and in comparison between aerobic and resistance training, more resistance training is more effective in reducing apoptotic factors and with increasing exercise intensity, SIRT1 is increased and it inhibits apoptotic factors.
Watkins PJ, Amiel SA, Howell SL, Turner E. Diabetes and its management: John Wiley & Sons; 2008.
Amos AF, McCarty DJ, Zimmet P. The rising global burden of diabetes and its complications: estimates and projections to the year 2010. Diabetic medicine. 1997;14(S5):S7-S85.
Uusitupa MI, Mustonen JN, Juhani Airaksinen K. Diabetic heart muscle disease. Annals of medicine. 1990;22(6):377-86.
Kirshenbaum L, Thomas T, Randhawa A, Singal P. Time-course of cardiac myocyte injury due to oxidative stress. Molecular and cellular biochemistry. 1992;111(1-2):25-31.
Oghbaei H, Asl NA, Sheikhzadeh F, Alipour MR. The effect of regular moderate exercise on miRNA-192 expression changes in kidney of streptozotocin-induced diabetic male rats. Advanced pharmaceutical bulletin. 2015;5(1):127.
Mejías-Peña Y, Estébanez B, Rodriguez-Miguelez P, Fernandez-Gonzalo R, Almar M, de Paz JA, et al. Impact of resistance training on the autophagy-inflammation-apoptosis crosstalk in elderly subjects. Aging (Albany NY). 2017;9(2):408.
McGarry JD. Banting lecture 2001: dysregulation of fatty acid metabolism in the etiology of type 2 diabetes. Diabetes. 2002;51(1):7-18.
Boden G. Role of fatty acids in the pathogenesis of insulin resistance and NIDDM. Diabetes. 1997;46(1):3-10.
Poitout V, Robertson RP. Minireview: secondary β-cell failure in type 2 diabetes—a convergence of glucotoxicity and lipotoxicity. Endocrinology. 2002;143(2):339-42.
Harmon JS, Gleason CE, Tanaka Y, Poitout V, Robertson RP. Antecedent hyperglycemia, not hyperlipidemia, is associated with increased islet triacylglycerol content and decreased insulin gene mRNA level in Zucker diabetic fatty rats. Diabetes. 2001;50(11):2481-6.
Evans JL, Goldfine ID, Maddux BA, Grodsky GM. Are oxidative stress− activated signaling pathways mediators of insulin resistance and β-cell dysfunction? Diabetes. 2003;52(1):1-8.
Schoenfeld BJ. The mechanisms of muscle hypertrophy and their application to resistance training. The Journal of Strength & Conditioning Research. 2010;24(10):2857-72.
Chu Q, Lee DT, Tsao SW, Wang X, Wong YC. S‐allylcysteine, a water‐soluble garlic derivative, suppresses the growth of a human androgen‐independent prostate cancer xenograft, CWR22R, under in vivo conditions. BJU international. 2007;99(4):925-32.
Zou H, Li Y, Liu X, Wang X. An APAF-1· cytochrome c multimeric complex is a functional apoptosome that activates procaspase-9. Journal of Biological Chemistry. 1999;274(17):11549-56.
Marzetti E, Calvani R, Bernabei R, Leeuwenburgh C. Apoptosis in skeletal myocytes: a potential target for interventions against sarcopenia and physical frailty–a mini-review. Gerontology. 2012;58(2):99-106.
Youle RJ, Strasser A. The BCL-2 protein family: opposing activities that mediate cell death. Nature reviews Molecular cell biology. 2008;9(1):47-59.
Hafstad AD, Boardman N, Aasum E. How exercise may amend metabolic disturbances in diabetic cardiomyopathy. Antioxidants & redox signaling. 2015;22(17):1587-605.
Jonker JT, de Mol P, de Vries ST, Widya RL, Hammer S, van Schinkel LD, et al. Exercise and type 2 diabetes mellitus: changes in tissue-specific fat distribution and cardiac function. Radiology. 2013;269(2):434-42.
Kanter M, Aksu F, Takir M, Kostek O, Kanter B, Oymagil A. Effects of low intensity exercise against apoptosis and oxidative stress in Streptozotocin-induced diabetic rat heart. Experimental and Clinical Endocrinology & Diabetes. 2017;125(09):583-91.
Santana ET, Serra AJ, Silva Junior JA, Bocalini DS, Barauna VG, Krieger JE, et al. Aerobic exercise training induces an anti-apoptotic milieu in myocardial tissue. Motriz: Revista de Educação Física. 2014;20(2):233-8.
Ignarro LJ, Balestrieri ML, Napoli C. Nutrition, physical activity, and cardiovascular disease: an update. Cardiovascular research. 2007;73(2):326-40.
Siddiqui, Aqeel, Abdi, Ahmad, Azarbayjani, Ali M., et al. The effect of aerobic exercise on some indicators of cardiac tissue apoptosis in male rats. Feyz Scientific Research Journal ::: Kashan University of Medical Sciences. 2019; 23 (5): 495-
Tanur maker, Saeed, Behpour, Nasser, Tadibi, Vahid. The effect of medium-term aerobic exercise on markers of apoptosis in the heart muscle cells of streptozotocin-induced diabetic rats. Journal of Fasa University of Medical Sciences. 2018; 7 (4): 488-97
Bjarnason-Wehrens B, Mayer-Berger W, Meister E, Baum K, Hambrecht R, Gielen S. Recommendations for resistance exercise in cardiac rehabilitation. Recommendations of the German Federation for Cardiovascular Prevention and Rehabilitation. European Journal of Cardiovascular Prevention & Rehabilitation. 2004;11(4):352-61.
Berent R, von Duvillard SP, Crouse SF, Sinzinger H, Green JS, Schmid P. Resistance training dose response in combined endurance-resistance training in patients with cardiovascular disease: a randomized trial. Archives of Physical Medicine and Rehabilitation. 2011;92(10):1527-33.
Yousef D, Farhad GS, Ghiassie R, Saeid S. Effect of resistance exercise on cardiac apoptosis following of ischemic/reperfusion. Journal of Animal and Veterinary Advances. 2011;10(19):2561-6.
Picoli CdC, Romero PVdS, Gilio GR, Guariglia DA, Tófolo LP, de Moraes SM, et al. Peak velocity as an alternative method for training prescription in mice. Frontiers in Physiology. 2018;9:42.
Baqersad Renani L, Melanouri Shamsi M, Mahdavi M, Qarakhanlu R, Mohammad Hassan Z, Zuhair. Effect of one-time resistance training on IL-15 mRNA expression in fast and slow skeletal direction of healthy and safe trained rats. Journal of Applied Sports Physiology. 2013; 9 (18): 15-26.
Davoodi SH, Vahidian-Rezazadeh M, Fanaei H. The effect of endurance and resistance exercises and consumption of hydro-alcoholic extract of nettle on the changes in weight and plasma levels of nesfatin-1 in type 1 diabetic rats. Feyz Journal of Kashan University of Medical Sciences. 2018;22(4):362-9.
Tanoorsaz S, Behpoor N, Tadibi V. Changes in Cardiac Levels of Caspase-8, Bcl-2 and NT-proBNP Following 4 Weeks of Aerobic Exercise in Diabetic Rats. International Journal of Basic Science in Medicine. 2017;2(4):172-7.
Ku Y, Han K, Ahn H, Kwon H, Koo B, Kim H, et al. Resistance exercise did not alter intramuscular adipose tissue but reduced retinol-binding protein-4 concentration in individuals with type 2 diabetes mellitus. Journal of international medical research. 2010;38(3):782-91.
Ramezani, Gaini, Choobineh, Kurdi, Hedayati. Changes in RBP-4 and insulin resistance after 8 weeks of aerobic exercise in male type 2 diabetic rats. Metabolism and exercise. 2017; 5 (2): 89-
Massi-Benedetti M, Herz M, Pfeiffer C. The effects of acute exercise on metabolic control in type II diabetic patients treated with glimepiride or glibenclamide. Hormone and metabolic research. 1996;28(09):451-5.
Esfahani M. Effect of physical training on blood glycemia, plasma insulin and cardiovascular risk factors in NIDDMs. Olympic games. 2007;14(4):17-24.
Segal KR, Edano A, Abalos A, Albu J, Blando L, Tomas MB, et al. Effect of exercise training on insulin sensitivity and glucose metabolism in lean, obese, and diabetic men. Journal of Applied Physiology. 1991;71(6):2402-11.
Cauza E, Hanusch-Enserer U, Strasser B, Ludvik B, Metz-Schimmerl S, Pacini G, et al. The relative benefits of endurance and strength training on the metabolic factors and muscle function of people with type 2 diabetes mellitus. Archives of physical medicine and rehabilitation. 2005;86(8):1527-33.
Ham O, Lee S-Y, Lee CY, Park J-H, Lee J, Seo H-H, et al. let-7b suppresses apoptosis and autophagy of human mesenchymal stem cells transplanted into ischemia/reperfusion injured heart 7by targeting caspase-3. Stem cell research & therapy. 2015;6(1):1-11.
Ghajari H, Hosseini SA, Farsi S. The effect of endurance training along with cadmium consumption on Bcl-2 and bax gene expressions in heart tissue of rats. Annals of Military and Health Sciences Research. 2019;17(1).
Liu J, Grundy SM, Wang W, Smith Jr SC, Vega GL, Wu Z, et al. Ten-year risk of cardiovascular incidence related to diabetes, prediabetes, and the metabolic syndrome. American heart journal. 2007;153(4):552-8.
Kazior Z, Willis SJ, Moberg M, Apró W, Calbet JA, Holmberg H-C, et al. Endurance exercise enhances the effect of strength training on muscle fiber size and protein expression of Akt and mTOR. PLoS One. 2016;11(2):e0149082.
Lee S-D, Kuo W-W, Wu C-H, Lin Y-M, Lin JA, Lu M-C, et al. Effects of short-and long-term hypobaric hypoxia on Bcl2 family in rat heart. International journal of cardiology. 2006;108(3):376-84.
Mooren F, Völker K. Molecular and cellular exercise physiology: Human Kinetics Publishers; 2005.
Peterson JM, Bryner RW, Sindler A, Frisbee JC, Alway SE. Mitochondrial apoptotic signaling is elevated in cardiac but not skeletal muscle in the obese Zucker rat and is reduced with aerobic exercise. Journal of applied physiology. 2008;105(6):1934-43.
Favaloro B, Allocati N, Graziano V, Di Ilio C, De Laurenzi V. Role of apoptosis in disease. Aging (Albany NY). 2012;4(5):330.
Koçtürk S, Kayatekin B, Resmi H, Açıkgöz O, Kaynak C, Özer E. The apoptotic response to strenuous exercise of the gastrocnemius and solues muscle fibers in rats. European journal of applied physiology. 2008;102(5):515-24.
Oh Y-S, Kwon H-Y, Jeong S-J, Park K-Y, Kim S-Y, Lee H-J, et al. Sojucktang induces apoptosis via loss of mitochondrial membrane potential and caspase-3 activation in KLE human endometrial cancer cells. Chinese Science Bulletin. 2009;54(23):4387-92.
Razavi Majd Z, Matin Homaee H, Azarbayjani M, Farzanegi P. Effects of concurrent regular aerobic training and garlic extract on cardiac tissue apoptosis markers in aged rats with chronic kidney disease. Journal of Medicinal Plants. 2017;2(62):46-54.
Petriz BA, Gomes CP, Almeida JA, de Oliveira Jr GP, Ribeiro FM, Pereira RW, et al. The effects of acute and chronic exercise on skeletal muscle proteome. Journal of cellular physiology. 2017;232(2):257-69.
Khakdan S, Delfan M, Heydarpour Meymeh M, Kazerouni F, Ghaedi H, Shanaki M, et al. High-intensity interval training (HIIT) effectively enhances heart function via miR-195 dependent cardiomyopathy reduction in high-fat high-fructose diet-induced diabetic rats. Archives of physiology and biochemistry. 2020;126(3):250-7.
Nourzad, F., Shahidi, F., & Saleh pourpour, M. (2022). The effect of aerobic and resistance training on insulin resistance index (HOMA-IR) and BCL-2/BAX ratio in apoptotic pathway in the heart tissue of male wistar diabetic rats. Journal of Sport and Exercise Physiology, 15(1), 69-82. doi: 10.52547/joeppa.15.1.69
MLA
Fatemeh Nourzad; Fereshteh Shahidi; Mojtaba Saleh pourpour. "The effect of aerobic and resistance training on insulin resistance index (HOMA-IR) and BCL-2/BAX ratio in apoptotic pathway in the heart tissue of male wistar diabetic rats". Journal of Sport and Exercise Physiology, 15, 1, 2022, 69-82. doi: 10.52547/joeppa.15.1.69
HARVARD
Nourzad, F., Shahidi, F., Saleh pourpour, M. (2022). 'The effect of aerobic and resistance training on insulin resistance index (HOMA-IR) and BCL-2/BAX ratio in apoptotic pathway in the heart tissue of male wistar diabetic rats', Journal of Sport and Exercise Physiology, 15(1), pp. 69-82. doi: 10.52547/joeppa.15.1.69
VANCOUVER
Nourzad, F., Shahidi, F., Saleh pourpour, M. The effect of aerobic and resistance training on insulin resistance index (HOMA-IR) and BCL-2/BAX ratio in apoptotic pathway in the heart tissue of male wistar diabetic rats. Journal of Sport and Exercise Physiology, 2022; 15(1): 69-82. doi: 10.52547/joeppa.15.1.69