Journal of Sport and Exercise Physiology

Journal of Sport and Exercise Physiology

Effects of Aerobic Training and Curcumin Supplementation on Blood Renalase, Creatinin and BUN Levels and Renal Damage in Male Rat Model of Induced Myocardial Infraction

Document Type : original article

Authors
Shiva Ghadirzad 1
Roghayeh Pouzesh Jadidi 1
Jabbar Bashiri 1
Karim Azali Alamdari 2
1 Department of Physical Education, Tabriz branch, Islamic Azad University, Tabriz, Iran
2 Department and Sport Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran
10.52547/joeppa.15.3.11
Abstract
Background and Purpose: The risk of renal injury rapidly increases following myocardial infraction (MI), however; aerobic training and curcumin supplementation have beneficial effects on both the kidneys and the heart post MI. Moreover, blood renalse level can also provide appreciable information regarding these organs health status. Therefore, the aim of study was to investigate the effects of aerobic training and curcumin supplementation on blood renalase, creatinin and blood ure nitrogen (BUN) levels and renal tubular damage in male rat model of induced myocardial infraction.
Material and Methods: 40 male wistar rats were randomized into five groups (n=8) of healthy control, MI control, MI training, MI curcumin and MI concomitant (training+curcumin). MI was induced by isoproterenol injection. The training program (consisted of five sessions of running/week at moderate intensity on a treadmill) was conducted throgh eight weeks and curcumin was administrated by gavage method 15 mg/kg.day. Blood renalse was determined by ELISA method, while creatinine and BUN level by enzymatic method and the tissue damge level was evaluated by histologic analysis. The data was analysed by one-way ANOVA (Tokey test as post hoc) and Chi square tests with a confidence level of 95%.
Results: Blood renalse (P = 0.001), creatinine (P = 0.001) and BUN (P = 0.025) was significantly higher in MI control group compared to healthy controls. However, the amounts of these indices had no significant differences with MI control group in all three intervention groups of MI training, MI curcumin and MI concomitant (P > 0.05). However; renal injury was significantly lower in all three intervention groups than MI control group (P = 0.007, P = 0.037 and P = 0.001 respecetively) and also in MI concomitant group compared to both of MI training (P = 0.006) and as well as MI curcumin (P = 0.003) groups.
Conclusion: The hazardous effects of MI on all three variables could be ameliorated following all three interventions. However, the concomitant intervention is superior to both of the aerobic training as well as curcumin supplementation for reductions in the histologic injury level. 
Keywords
Myocardial Infraction
Training
Curcumin
Renal Injury
  1. Fathima SN. An Update on Myocardial Infarction. Current Research and Trends in Medical Science and Technology. 2021:1.
  2. Lobo Filho HG, Ferreira NL, de Sousa RB, de Carvalho ER, Lobo PLD, Lobo Filho JGJRbdccoodSBdCC. Experimental Model of Myocardial Infarction Induced by Isoproterenol in Rats. 2011;26(3):469-76.
  3. Schiffrin EL, Lipman ML, Mann JF. Chronic kidney disease: effects on the cardiovascular system. Circulation. 2007;116(1):85-97.
  4. Bansal N, Katz R, Robinson-Cohen C, Odden MC, Dalrymple L, Shlipak MG, et al. Absolute rates of heart failure, coronary heart disease, and stroke in chronic kidney disease: an analysis of 3 community-based cohort studies. JAMA cardiology. 2017;2(3):314-8.
  5. Wu Y, Wang L, Wang X, Wang Y, Zhang Q, Liu W. Renalase contributes to protection against renal fibrosis via inhibiting oxidative stress in rats. International urology and nephrology. 2018;50(7):1347-54.
  6. Li X, Xie Z, Lin M, Huang R, Liang Z, Huang W, et al. Renalase protects the cardiomyocytes of Sprague-Dawley rats against ischemia and reperfusion injury by reducing myocardial cell necrosis and apoptosis. Kidney and Blood Pressure Research. 2015;40(3):215-22.
  7. Li Y, Wu W, Liu W, Zhou M. Roles and mechanisms of renalase in cardiovascular disease: A promising therapeutic target. Biomedicine & Pharmacotherapy. 2020;131:110712.
  8. Desir GV, Wang L, Peixoto AJ. Human renalase: a review of its biology, function, and implications for hypertension. Journal of the American Society of Hypertension. 2012;6(6):417-26.
  9. He B, Hao J, Sheng W, Xiang Y, Zhang J, Zhu H, et al. Correlation between plasma renalase level and coronary artery disease. Pakistan journal of medical sciences. 2014;30(5):863.
  10. Gu R, Lu W, Xie J, Bai J, Xu B. Renalase deficiency in heart failure model of rats—a potential mechanism underlying circulating norepinephrine accumulation. PloS one. 2011;6(1):e14633.
  11. Xing Y, Yang S-D, Wang M-M, Feng Y-S, Dong F, Zhang F. The beneficial role of exercise training for myocardial infarction treatment in elderly. Frontiers in physiology. 2020;11:270.
  12. Wu F, Li Z, Cai M, Xi Y, Xu Z, Zhang Z, et al. Aerobic exercise alleviates oxidative stress-induced apoptosis in kidneys of myocardial infarction mice by inhibiting ALCAT1 and activating FNDC5/Irisin signaling pathway. Free Radical Biology and Medicine. 2020;158:171-80.
  13. Safdar B, Guo X, Johnson C, D'Onofrio G, Dziura J, Sinusas AJ, et al. Elevated renalase levels in patients with acute coronary microvascular dysfunction–a possible biomarker for ischemia. International journal of cardiology. 2019;279:155-61.
  14. Czarkowska-Paczek B, Zendzian-Piotrowska M, Gala K, Sobol M, Paczek L. Exercise Differentially Regulates Renalase Expression in Skeletal Muscle and Kidney. The Tohoku Journal of Experimental Medicine. 2013;231(4):321-9.
  15. de Almeida Alvarenga L, de Oliveira Leal V, Borges NA, de Aguiar AS, Faxén-Irving G, Stenvinkel P, et al. Curcumin-A promising nutritional strategy for chronic kidney disease patients. Journal of Functional Foods. 2018;40:715-21.
  16. Boarescu P-M, Boarescu I, Bulboacă AE, Bocșan IC, Pop RM, Gheban D, et al. Multi-Organ Protective Effects of Curcumin Nanoparticles on Drug-Induced Acute Myocardial Infarction in Rats with Type 1 Diabetes Mellitus. Applied Sciences. 2021;11(12):5497.
  17. Boarescu P-M, Chirilă I, Bulboacă AE, Bocșan IC, Pop RM, Gheban D, et al. Effects of curcumin nanoparticles in isoproterenol-induced myocardial infarction. Oxidative medicine and cellular longevity. 2019;2019.
  18. Xiao J, Sheng X, Zhang X, Guo M, Ji X. Curcumin protects against myocardial infarction-induced cardiac fibrosis via SIRT1 activation in vivo and in vitro. Drug design, development and therapy. 2016;10:1267.
  19. Moulin S, Arnaud C, Bouyon S, Pépin J-L, Godin-Ribuot D, Belaidi E. Curcumin prevents chronic intermittent hypoxia-induced myocardial injury. Therapeutic Advances in Chronic Disease. 2020;11:2040622320922104.
  20. Fan Y, Chen H, Peng H, Huang F, Zhong J, Zhou J. Molecular Mechanisms of Curcumin Renoprotection in Experimental Acute Renal Injury. 2017;8(912).
  21. Ali BH, Al-Salam S, Al Suleimani Y, Al Kalbani J, Al Bahlani S, Ashique M, et al. Curcumin Ameliorates Kidney Function and Oxidative Stress in Experimental Chronic Kidney Disease. 2018;122(1):65-73.
  22. Ueki M, Ueno M, Morishita J, Maekawa NJJob, bioengineering. Curcumin ameliorates cisplatin-induced nephrotoxicity by inhibiting renal inflammation in mice. 2013;115(5):547-51.
  23. Sun X, Li C, Lu J. Protective effect of ginsenoside Rd against isoproterenolinduced myocardial infarction in Wistar rats. Tropical Journal of Pharmaceutical Research. 2019;18(1):93-100.
  24. Zhou Y, Li M, Song J, Shi Y, Qin X, Gao Z, et al. The cardioprotective effects of the new crystal form of puerarin in isoproterenol-induced myocardial ischemia rats based on metabolomics. Scientific reports. 2020;10(1):1-18.
  25. Tofighi A, Ebrahimi Kalan A, Jamali Qarakhanlou B. The effect of resveratrol supplementation and aerobic training on cardiac tissue alteration of rats with acute myocardial infarction. Iranian Journal of Physiology and Pharmacology. 2018;1(4):221-11.
  26. Majidi A, Poozesh Jadidi R, Azali Alamdari K, Bashiri J, Nourazar MAR. Effects of Aerobic Training and Curcumin Supplementation on Cardiomyocyte Apoptosis and MiRNAs Expression in Rats Exposed to Arsenic. Sport Physiology. 2020;12(48):39-60.
  27. Hosseinlou A, Pouzesh Jadidi R, Bashiri J, Nourazar MAR, Azali- Alamdari K. Effects of High Intensity Interval Training and Curcumin Supplementation on Hippocampal Total Antioxidant Capacity, GRP78 and Caspase 3 Level in Male Rats Exposed to Arsenic. Journal of Applied Exercise Physiology. 2020;16(32):15-29.
  28. Demirbilek S, Karaman A, Baykarabulut A, Akin M, GÜRÜNLÜOǦLU K, Türkmen E, et al. Polyenylphosphatidylcholine pretreatment ameliorates ischemic acute renal injury in rats. International journal of urology. 2006;13(6):747-53.
  29. Kaltsas E, Chalikias G, Tziakas D. The incidence and the prognostic impact of acute kidney injury in acute myocardial infarction patients: current preventive strategies. Cardiovascular drugs and therapy. 2018;32(1):81-98.
  30. Huang Z, Li Q, Yuan Y, Zhang C, Wu L, Liu X, et al. Renalase attenuates mitochondrial fission in cisplatin-induced acute kidney injury via modulating sirtuin-3. Life sciences. 2019;222:78-87.
  31. Marenzi G, Assanelli E, Campodonico J, De Metrio M, Lauri G, Marana I, et al. Acute kidney injury in ST-segment elevation acute myocardial infarction complicated by cardiogenic shock at admission. Critical care medicine. 2010;38(2):438-44.
  32. Moningka NC, Cunningham J, Mark W, Sterling M, West CA, Verlander JW, Croker BP, et al. Effects of voluntary wheel running on the kidney at baseline and after ischaemia–reperfusion‐induced acute kidney injury: a strain difference comparison. The Journal of physiology. 2013;591(5):1313-24.
  33. Tokinoya K, Ono S, Aoki K, Yanazawa K, Shishikura Y, Sugasawa T, et al. Gene expression level of renalase in the skeletal muscles is increased with high-intensity exercise training in mice on a high-fat diet. Physiology International. 2021;108(2):274-84.
  34. Rodrigues B, Lira FS, Consolim-Colombo FM, Rocha JA, Caperuto EC, De Angelis K, et al. Role of exercise training on autonomic changes and inflammatory profile induced by myocardial infarction. Mediators of inflammation. 2014;2014.
  35. Mokhtari‐Zaer A, Marefati N, Atkin SL, Butler AE, Sahebkar A. The protective role of curcumin in myocardial ischemia–reperfusion injury. Journal of cellular physiology. 2019;234(1):214-22.
  36. Boulanger CM, Scoazec A, Ebrahimian T, Henry P, Mathieu E, Tedgui A, et al. Circulating microparticles from patients with myocardial infarction cause endothelial dysfunction. Circulation. 2001;104(22):2649-52.
  37. Ostrowski SR, Pedersen SH, Jensen JS, Mogelvang R, Johansson PI. Acute myocardial infarction is associated with endothelial glycocalyx and cell damage and a parallel increase in circulating catecholamines. Critical care. 2013;17(1):1-12.
  38. Zouhal H, Jacob C, Delamarche P, Gratas-Delamarche A. Catecholamines and the Effects of Exercise, Training and Gender. Sports Medicine. 2008;38(5):401-23.
  39. Saied NM, Georgy GS, Hussien RM, Hassan WA. Neuromodulatory effect of curcumin on catecholamine systems and inflammatory cytokines in ovariectomized female rats. Clinical and Experimental Pharmacology and Physiology. 2021;48(3):337-46.
  40. Stuhr LE, Ask JA, Tyssebotn I. Inotropic and chronotropic effects of isoprenaline in rats exposed to 30 bar. Aviation, space, and environmental medicine. 1991;62(1):41-5.
  41. Wang C, Pei Y-Y, Ma Y-H, Ma X-L, Liu Z-W, Zhu J-H, et al. Risk factors for acute kidney injury in patients with acute myocardial infarction. Chinese medical journal. 2019;132(14):1660.
  42. Pradet-Balade B, Boulmé F, Beug H, Müllner EW, Garcia-Sanz JA. Translation control: bridging the gap between genomics and proteomics? Trends in biochemical sciences. 2001;26(4):225-9.
  43. Ali BH, Abdelrahman A, Al Suleimani Y, Manoj P, Ali H, Nemmar A, et al. Effect of concomitant treatment of curcumin and melatonin on cisplatin-induced nephrotoxicity in rats. Biomedicine & Pharmacotherapy. 2020;131:110761.
  44. Du M, Huang K, Huang D, Yang L, Gao L, Wang X, et al. Renalase is a novel target gene of hypoxia-inducible factor-1 in protection against cardiac ischaemia–reperfusion injury. Cardiovascular Research. 2015;105(2):182-91.
  45. Aili N, Qing L, Shuo Z, Yushuang C, Shulin Q. Protective Effect of Curcumin on Renal Injury In High-intensity Exercise Rats and Its Mechanisms. Journal of Hunan Normal University (Medical Sciences). 2012:01.
  46. Hai-tao Z, Jian-min C, Sheng-quang M, Jing Z, Hao L. Effects of Curcumin on the Protein and Gene Expression of Inflammatory Cytokines in Renal Tissue of Rat with Exercise-related Renal Ischemia-reperfusion Injury. Natural Product Research and Development. 2018;30(10):1687.
  47. Chainani-Wu N. Safety and anti-inflammatory activity of curcumin: a component of tumeric (Curcuma longa). The Journal of Alternative & Complementary Medicine. 2003;9(1):161-8.

 

  • Receive Date 05 September 2021
  • Revise Date 30 November 2021
  • Accept Date 28 December 2021
  • First Publish Date 21 June 2022
  • Publish Date 22 June 2022