The effect of six weeks High Intensity Interval Swimming Training and Resveratrol supplementation on the level of SIRT3 in left ventricular heart of aged rats

Document Type : original article


1 Faculty of Physical Education and Sport Sciences, University of Tehran Kish International Campus, Kish, Iran

2 Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran


Background and Purpose: Identification and analysis of compounds that playing a role in delay aging and increase longevity is essential. Sirtuins are one of the main regulators of cell survival and longevity. The goal of this research was to investigate the effect of six weeks HIIT swimming exercise and Resveratrol supplementation on the level of SIRT3 in left ventricular heart of aged rats.
Materials and Methods: In this research, 30 wistar rats were randomly divided into five groups (control (C), solvent (M), supplement (R), HIIT exercise (EX), HIIT exercise and supplement (EXR)). The EX group performed HIIT swimming for six weeks. C group did not practice. R group received only resveratrol supplementation. EXR group performed HIIT swimming exercises with resveratrol and M group received only solvent. The SIRT3 protein was obtained by Western blotting. Data were analyzed using One-way ANOVA analysis of variance and Tukey's post hoc test.
Results: After six weeks, there was a significant difference between the mean SIRT3 protein levels in the five groups. Therefore, Tukey post hoc test showed that SIRT3 levels were significantly increased in EX and EXR groups compared to C, R and M groups (P = 0/001). However, this difference between EX and EXR groups was not significant (P > 0.05). Moreover, the amount of SIRT3 in group R was significantly higher compared to groups C and M.
Conclusion: Increasing the amount of SIRT3 indicates the involvement of this protein in metabolic pathways, antioxidant defense and improves cell condition. High intensity interval training with resveratrol supplement consumption can also be used to moderate oxidative stress and delay the aging process.


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  • Receive Date: 15 November 2021
  • Revise Date: 29 December 2021
  • Accept Date: 01 January 2022
  • First Publish Date: 21 June 2022
  • Publish Date: 22 June 2022