The effect of 8 weeks of High Intensity Interval Training on the Levels of Wnt and NF-κB proteins in the heart tissue of male Wistar rats with type 2 diabetes

Document Type : original article


1 Department of Sport Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Sport Physiology, Neuromuscular Orientation, Fasa, Iran

3 Department of Physiology and Pharmacology, Afzalipour Medical Faculty, Physiology Research Center and Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran


Background and Purpose: Diabetic cardiomyopathy is one of the main risk factors for cardiovascular complications of diabetes and since physical activity in diabetic patients can affect the structure and function of the myocardium, the aim of the present study was to investigate the effect of eight weeks of HIIT training on the content of NF-kB and Wnt proteins in the heart muscle tissue of Wistar rats with type 2 diabetes.
Materials and Methods: In the present study, 20 male Wistar rats were randomly divided into four groups (each group including five rats) Control+Healthy, Exercise+Healthy, Control+Diabetes, and Exercise+Diabetes. After a period of high-fat diet and then after induction of diabetes, animals in Exercise+Diabetes and Exercise+Healthy groups performed the training protocol for eight weeks and five sessions per week. 48 hours after the last training session, cardiac tissue was extracted and the expression levels of NF-kB and Wnt proteins were assessed using Western blotting. Data were analyzed using one-way ANOVA and Tukey post hoc test at a significance level of less than 0.05. Also, mixed ANOVA test was used to evaluate changes in weight and glucose levels of rats in different stages and weeks of training.
Results: The results showed that the relative Levels of NF-kB and Wnt proteins in Control+Diabetes and Exercise+Eiabetes groups is significantly more than healthy groups (P = 0.001). Also, the Levels of Wnt protein in the Exercise+Diabetes group was significantly less than the Control+Diabetes group (P = 0.001), While the Levels of NF-κB protein in the Exercise+Diabetes group did not show a significant difference compared to the Control+Diabetes group. The results of mixed ANOVA test on mean weight changes show that the weight of rats in Control+Diabetes and Exercise+Diabetes groups was significantly higher (P = 0.001) than the beginning of training and use of high-fat diet to the stage before STZ injection and in the last week. In addition, at the end of the training protocol was significantly became less (P = 0.001). Also, blood glucose levels of Control+Diabetes and Exercise+Diabetes groups was significantly higher (P = 0.001 and 0.003, respectively) at one week after STZ injection and was significantly lower in the Exercise+Diabetes group at the end of the training protocol (P=0.001).
Conclusion: Finally, it should be noted that high-intensity intermittent exercise could potentially affect myocardial structure and function and be promising as a non-pharmacological solution.


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Volume 15, Issue 4 - Serial Number 31
September 2022
Pages 19-30
  • Receive Date: 25 March 2022
  • Revise Date: 01 May 2022
  • Accept Date: 22 May 2022
  • First Publish Date: 10 August 2022
  • Publish Date: 23 August 2022