Document Type : original article
1 Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
2 .Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
Background and Purpose: Pathogenic cardiac hypertrophy is a type of heart disorder that is associated with changes in the structure of the heart, apoptosis and fibrosis of the heart muscle cells, which leads to a decrease in the pumping ability of the heart and may eventually lead to heart failure and sudden death. It has been shown that the signaling pathway of transcription factors NF-κB and NFAT play an important role in the process of pathogenic hypertrophy of the heart, and considering that few studies have investigated the effect of exercise on these factors in the process of pathogenic hypertrophy,the aim of the present study was to identify the effect of eight weeks of aerobic exercise before isoproterenol induction on the expression of NF-κB and NFAT genes in the left ventricular tissue of male Wistar rats.
Materials and Methods: 18 male Wistar rats were randomly divided into two groups of endurance training and control. Endurance training was performed for eight weeks, one hour a day and six days a week on a treadmill with a slope of 15 degrees. After eight weeks of training, pathological hypertrophy was induced by subcutaneous injection of three mg / kg isoprenaline for seven days. 24 hours after the last injection session, rats were anesthetized by intraperitoneal injection of 50 mg ketamine and 10 mg xylazine. The rats were then dissected and cardiac tissue was extracted and transferred to -70 ° C. Also, in the present study, Real Time PCR was used to evaluate the expression of NF-κB and NFAT genes. Data analysis was performed using SPSS software version 24 and independent t-test at a significance level of P ≥ 0.05
Results: The results of the present study showed that the indices of heart weight, heart weight to body weight ratio (H / W) and left ventricular weight to body weight ratio (V / W) were significantly (P = 0.01) and (P = 0.03): Respectively, increased in the exercise group compared to the control group. Also, the relative expression of NF-κB gene was significantly (P = 0.03) decreased in the exercise group compared to the control group, while the relative expression of NFAT gene was not significantly changed in the study groups (P = 0.40).
Conclusion: Eight weeks of aerobic endurance training may play an important role in the prevention of pathological cardiac hypertrophy by reducing the relative expression of the NF-κB gene.
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