Response of Klotho and PLP proteins to high-intensity interval training in hippocampus and cerebellum tissues of female mice

ALVANDI, MASOOMEH; Salimi, Kia; Farahmand, Fataneh; Nourshahi, Maryam

doi:10.48308/joeppa.2024.237261.1304

Response of Klotho and PLP proteins to high-intensity interval training in hippocampus and cerebellum tissues of female mice

Articles in Press

Document Type : Original Article

Authors

¹ exercise physiology physical education faculty Shahid Beheshti university Tehran Iran

² Department of Exercise Physiology, Faculty of Sport and Exercise Sciences, University of Tehran, Tehran, Iran

³ Department of Kinesiology, McMaster University, Hamilton, Canada

⁴ Department of Biological Sciences in Sport and Health, Faculty of Sports Sciences and Health, Shahid Beheshti University, Tehran, Iran

10.48308/joeppa.2024.237261.1304

Abstract

Abstract

Background and Purpose: Exercise, as a well-known method without the potential side effects associated with pharmacological interventions, has multiple beneficial effects on neurobiological function. Recent studies have shown that exercise increases neurological pathways in the brain and by increasing myelin-forming biomarkers, exercise can improve brain function and health and reduce the risk of neurodegenerative disease. The present study aimed to investigate the effect of 8 weeks of high-intensity interval training (HIIT) on the levels of Klotho (a regulator of remyelination and oligodendrocyte maturation), PLP (proteolipid protein, one of the major myelin proteins) in the hippocampus and cerebellum tissue of mice.
Materials and Methods: Twenty female C57BL/6 mice (6 weeks old with an average weight of 15 ± 2 g) were randomly divided into control (n=10) and exercise (n=10) groups. The exercise group performed five sessions of high-intensity interval training per week for 8 weeks. The protocol initially consisted of, 1) a 5-minute warm-up at an intensity of 30-40 % Vmax; 2) two-minute interval repetitions at an intensity of 80-110 % Vmax and active recovery at an intensity of 30-40 % Vmax), and 3) a cool-down at an intensity of 30-40% Vmax. Forty-eight hours after the last training session, Klotho and PLP levels in the hippocampus and cerebellum tissue were evaluated using Western blot technique. Data were analyzed using SPSS statistical software with v21. An independent samples t-test was used to evaluate the difference between Klotho and PLP levels in exercise and control groups. Significance level was accepted at P≤0.05.
Results: The results of the present study showed that the amount of Klotho in the hippocampus tissue increased in the exercise group (p<0.01), but the concentration of PLP in the hippocampus did not change (p=0.4). In addition, the results showed that, the concentration of Klotho and PLP in cerebellum tissue increased in the exercise group compared with the control group (p ≤ 0.01, p ≤ 0.05, respectively).
Conclusions: Our findings showed for the first time that high-intensity interval training increased Klotho in hippocampus tissue. We also showed that this type of training increased Klotho and PLP in cerebellum tissue compared to the control group, and may therefore improve Klotho and PLP levels that typically decline with aging and disease. The current work provides proof of concept that HIIT exercise can be considered a model for increasing brain levels of Klotho and PLP proteins, which has the potential to positively impact brain structure and function.

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