Despite of injurious effects of plyometric training, it is possible to use the stretch training for attenuating of degenerative consequences of plyometric exercises. The purpose of this study was to investigate the effect of static, dynamic and combined stretching on blood lactate and delayed muscle soreness immediately, 24 and 48 hours after of one acute plyometric exercise session. 46 untrained males with a mean age of 16.62±1.05 years, height 169.89±5.64 cm, weight 56.73±5.04 kg, and body mass index of 19.72±1.64 kg٫m², were randomly assigned into static, dynamic, and combined stretching groups and a non-stretching one. After completing the 7±1 minutes of stretching by traning groups, it is periscribed a plyometric exercise protocol. Blood lactate evaluated before and immediately after exercise protocol. Also, delayed muscle soreness in pre-, and 24 and 48 h after plyometric session were measured using Robinson's 10-point questionnaire. It is applied the paired t, analysis of variance and Tukey tests at a significance level of (P<0.05) for extraction of results. Although blood lactate showed a significant increases after exercise in four groups, but its elevation was significantly (P-0.02) lower in the dynamic than non-stretching group. Delayed muscle soreness significantly increased 24 and 48 hours after plyometric protocol as well. In addition, It is observed no significantly differences in muscle soreness 24 h after exercise, while it was only significantly lower (P=0.01) in dynamic group 48 h after plyometric exercise. Generally, comparing to static and combined stretch training, performing of dynamic stretching before plyometric exercise will be along with lower blood lactate accumulation and muscle soreness.