The effect of acute consumption of HMB and creatine supplement on oxidative and antioxidant indices after resistance exercise in trained men

Document Type : original article

Abstract

Introduction: The aim of this study was to determine the acute effect of HMB and Creatine supplementation on the oxidant and antioxidant indices on trained men after resistance exercise.
Method: 10 active men with a mean age of 20±1.15 years, height: 172.2±4.82 cm, weight: 70.9±9.66 kg and body mass index: 24.04±2.44 kg/m2 was purposefully selected and then in a crossover clinical trial design was randomly divided into 4 groups. Resistance exercises including bench press, leg extensions, barbell curl, triceps extension, shoulder press and lat pull down were carried out at 80% 1RM. Prior to each resistance exercise session, subjects were randomized to Creatine, Creatine+HMB, HMB or placebo loading for 5 days. One-week washout was considered between supplementation periods. A day after the completion of each supplementation courses, blood samples were taken in the fasting state to evaluate the effect of supplementation on current research variables.
Results: The results using ANOVA with repeated measurements showed that ingestion of creatine, HMB, or combined supplements has no effect on the serum levels of uric acid, bilirubin, malondialdehyde and glutathione peroxidase.
Conclusion: Overall, the present research findings suggest that short term (5 days) creatine, HMB and combined Cr + HMB has no effect on the oxidant and antioxidant variations following acute resistance exercise.

Keywords

References

  1. - Braun, H., Koehle, K., Geyer, H., Kleiner, J., Master, J. et al. (2009). Dietary supplement use among elite young German athletes. Int J Sport Nutr Exerc Metab.19 (1): 97- 109.
  2. - Fisher, W.K., Bloomer, R. (2009). Acute exercise and oxidative stress: a 30-year history. Dynamic Medicine. 8: 1-25.
  3. - Goldfarb, A., McKenzie, M., Bloomer, R. (2007). Gender comparisons of exercise-induced oxidative stress: influence of antioxidant supplementation. Applied Physiology, Nutrition, and Metabolism. 32: 1124-1131.
  4. - Konig, D., Wagner., KH., Elmadfa, l., Berg, A. (2001). Exercise and oxidative stress: Significance of antioxidants with reference to inflammatory. Exerc Immunol Rev. 7 :108-33.
  5. - Wang, SH.Y., Ballington, J.R. (2007). Free radical scavenging capacity and antioxidant enzyme activity in deerberry (vaccinium stamineum l). LWT. 40: 1352-1361.
  6. - Maughan, RJ., Depiesse, F., Geyer, H. (2007). The use of dietary supplements by athletes. J Sports Sci. 25 (1): 103- 113.
  7. - Wilson, J.M., Fitschen, P.J., Cmpbell, B., Wilson, G.J., Zanchi, N., Tylor, L. et al. (2013). International society of sports nutrition stand: beta-hydroxi-beta methylbutyrate (HMB). J Int Soc Sports Nutr. DOI: 10.1186/1550-2783-10-6.
  8. - Van Koevering, MT., Dolezal, HG., Gill, DR., Owens, FN., Strasia, CA., Buchanan, DS., et al. (1994). Effects of beta- hydroxi-beta-methyl butyrate on performance and carcass quality of feedlot steers. J Anim Sci. 72(8): 1927-1935.
  9. - Anthony, JC., Yoshizawa, F., Anthony, TG., Vary, TC., Jefferson, LS., Kimball, SR. (2000). Leucine stimulates translation initiation in skeletal muscle of post absorptive rats via a rapamycin-sensitive pathway. J Nutr, 130 (10):2413–2419.
  10. - Howatson,G., Hoad, M., Goodall, S., Tallent, J., Bell, PG., French, DN. (2012). Exercise-induced muscle damage is reduced in resistance-trained males by branched chain amino acids: a randomized, double-blind, placebo controlled study. J Int Soc Sports Nutr. DOI: 10.1186/1550-2783-9:20.
  11. - Heros Ribeiro Ferreira, Ander luize flix rodacki and et al. (2013). The effects of supplementation of beta- hydroxy- beta- methylbutyrate on inflammatory markers in high performance athletes. 36: 327- 358.
  12. - Rahimi, R. (2011). Creatine supplementation decreases oxidative DNA damage and lipid peroxidation induced by a single bout of resistance exercise. J Strength Cond Res. 25(12): 3448- 55.
  13. - Deminice, R., Rosa, F.T., Franco, G.S., Jordao, A.A., De Freitas, E.C. (2013). Effects of creatine supplementation on oxidative stress and inflammatory markers after repeated- sprint exercise in humans. Nutrition. 29(9): 1127- 32.
  14. - Kramer, W.J., Hatfield, D.L., Volek, J.S., Fragala, M.S., Vingren, J.L., Anderson, J.M. et al. (2009). Effects of amino acids supplement on physiological adaptations to resistance training. Med Sci Sports Exerc. 41(5): 1111- 1121.
  15. - Heyward, V.H., and Gibson, a.l. (1991). Advanced fitness assessment & exercise prescription. Champing IL: human kinetic co, 7th edition.
  16. - Thomas, L. (1998). Clinical laboratory diagnostics. 1sted. Frankfurt: TH-Books verlagsgesellschaft. 208- 14.
  17. -Rand, RN., and DI Pasqua, A. (1962). A new diazo method for the determination of bilirubin. Clin Chem. 8:570-8.
  18. - Basta, P., Skarpanska- Stejnborn, A., Pilaczynska, L. (2006). Creatine supplementation and parameters of exercise-induced oxidative stress after a standard rowing test. Studies in physical culture and tourism. 13 (1): 17- 23.
  19. - Araujo, M.B., Moura, L.P., Junior, R.C., Junior, M.C., Dalia, R.A., Sponton, A.C. et al. (2013). Creatine supplementation and oxidative stress in rat liver. DOI: 10.1186/1550-2783-: 10-54.
  20. - Percario, S.P., Domingue, S., Teixeira, L.F., Fernandes, J.L., de Vasconcelos, F., Ciarrocchi, D.M. et al. (2012). Effects of creatine supplementation on oxidative stress profile of athletes. J Int Soc Sports Nutr. DOI: 10.1186/1550-2783-: 9-56.
  21. - میرزایی، بهمن؛ رحمانی نیا، فرهاد؛ صالحی، زیور؛ رحیمی، رحمان. (1391). اثر فعالیت هوازی و مصرف مکمل کراتین مونوهیدرات
  22. بر استرس اکسیداتیو و ترکیب بدن در ورزشکاران. فیزیولوژی ورزشی، شماره 16، 97-118.
  23. - Lucas Guimaraes, Carlos herman, J., Pinheiro, Frederico Gerlinger, Romero, et al. (2012). Short-term creatine supplementation decreases reactive oxygen species content with no changes in expression and activity of antioxidant enzymes in skeletal muscle. Eur J Appl physiol. 112: 3905- 3911.
  24. - Eley, H.L., Russell, S.T., and Tisdale, M.J. (2008). Mechanism of attenuation of muscle protein degradation induced by tumor necrosis factor-α and angiotensin II by β-hydroxy-β-methyl butyrate. Am J Physiol Endocrinol Metab. E1417-E1426.

Files

History

  • Receive Date: 13 August 2016
  • Revise Date: 19 June 2024
  • Accept Date: 31 December 2020
  • First Publish Date: 31 December 2020
  • Publish Date: 22 May 2017

Share

How to cite

Statistics