Effects of aerobic training and black carbon particles exposure on gene expression of NF-κB and TNF-α of hippocampus brain tissue of male rats

Document Type : Original Article


1 Faculty of Physical Education and Sport Science, Tarbiat Modaress University, Tehran, Iran

2 Faculty of Physical Education & Sport Sciences, Shahid Beheshti University, Tehran, Iran

3 Department of Exercise Physiology, Zanjan University, Zanjan, Iran



Purpose: The purpose of this study was to investigate the effect of aerobic exercise and black carbon particles
on gene expression of NF-κB and TNF-α in rats.
Methods: Twenty-four male Wistar rats with an average body mass 279.29±26.97 g were randomly divided
into 4 groups A: without exposure PM10 and aerobic exercise, B: aerobic training, C: exposure to PM10
carbon black (5 mg/m3; per rat), D: and aerobic exercise concomitantly with exposure to PM10 carbon black.
Twenty-four hours after the last training session and exposure, the animals were sacrificed. Real timePcr technique was used. Two-way ANOVA and post hoc LSD was used to determine the significance of
differences between groups.
Results: There was significant difference between groups A and B (P=0.001) and B and D (P=0.003) in the
amount of TNF-α. Significant difference between A and B groups (P=0.008) in the amount of NF-κB was
Conclusions: four weeks of aerobic exercise and PM10 exposure lead to modify the inflammatory factors of
NF-κB and TNF-α. It seems that these changes lead to reducing the risk of neurodegenerative diseases. 


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  • Receive Date: 25 April 2016
  • Revise Date: 06 October 2016
  • Accept Date: 26 December 2016
  • First Publish Date: 23 August 2018
  • Publish Date: 23 August 2018