Journal of Sport and Exercise Physiology

Journal of Sport and Exercise Physiology

The effect of vigorous continuous and interval exercise training along with resveratrol on SIRT3 and OGG1 proteins in the liver tissue of male Wistar rats

Document Type : original article

Authors
Mohammad Esmaeil Afzalpour 1
Hadi Sarir 2
Zoufa Zanjirian 1
Mohsen Moammadnia Ahmadi 1
ELHAM GHASEMI 1
1 Department of Sport Sciences, Faculty Sport Sciences, University of Birjand, Iran
2 Department of Animal Sciences, Facult of Agriculture, University of Birjand, Iran
10.52547/joeppa.13.1.111
Abstract
Purpose: Sirtuins-3 (SIRT3) is a mitochondrial deacetylase and through acetylation of Oxoguanine Glycosylase 1(OGG1) protein caused repair mitochondrial DNA damage and prevents cell apoptosis. The purpose of this study was to evaluate the effect of vigorous continuous and interval exercise training along with resveratrol on SIRT3 and OGG1 proteins in the liver tissue of male Wistar rats.
Methods: In this research, forty-eight male Albino Wistar rats divided into six equal of eight groups including sedentary control, supplement, interval exercise, interval exercise + supplement, continuous training and continuous training+ supplement. Continuous and interval training performed for 6 weeks, six days per week at speed of 27 and 54 m/min respectively on a rat treadmill. Resveratrol users received daily 50 mg/kg of body weight supplements as gavage.  After intervention, the animals were anesthetized and their liver samples were analysed using one-way ANOVA and Tukey's post hoc tests.
Results: After 6 weeks OGG1 level was significantly increased in continuous exercise (p=0.0001) and continuous training + supplement (p = 0.0001) groups relative to the control group. Also, liver tissue SIRT3 levels in supplementation group (p = 0.01), interval training group (p = 0.04), interval training + supplement group (p = 0.04), continuous exercise group (p = 0.001), and continuous training + supplement group (P = 0.001) increased than control group after six weeks. 
Conclusion: Compare to the interval training, performing of continuous training along with resveratrol consumption can induce more improvement in cell survival and life-span factors such as SIRT3 and OGG1. 
Keywords
Resveratrol
Sirtuins-3
Oxoguanine Glycosylase -1
Continuous and Intensity Interval training
  1. Afzalpour ME, Chadorneshin HT, Foadoddini M, Eivari HA. Comparing interval and continuous exercise training regimens on neurotrophic factors in rat brain. Physiol Behav. 2015; 147: 78-83
  2. Tejero J, Shiva S, Gladwin MT. Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation. Physiol Rev. 2019; 99(1):311-379.
  3. Afzalpour ME, Ghasemi E, Zarban A. Effects of 10 Weeks of High Intensity Interval Training and Green Tea Supplementation on Serum Levels of Sirtuin-1 and Peroxisome Proliferator-Activated Receptor Gamma Co-activator 1-Alpha in Overweight Women. Sci & Sports. 2017; 32: 82-90.
  4. Brandauer J, Andersen MA, Kellezi H, Risis S, Frøsig C, Vienberg SG, et al. AMP-activated protein kinase controls exercise training-and AICAR-induced increases in SIRT3 and MnSOD. Front Physiol. 2015; 6: 85.
  5. Haigis MC, Guarente LP. Mammalian Sirtuins-emerging roles in physiology, aging, and calorie restriction. Genes Dev. 2006; 20 (21):2913-2921.
  6. Palacios OM, Carmona JJ, Michan S, Chen KY, Manabe Y, Ward Jr, et al. Diet and exercise signals regulate SIRT3 and activate AMPK and PGC-1alpha in skeletal muscle. Aging (Albany NY). 2009; 1 (9): 771-783.
  7. Chen Y, Fu L, Wen X, Wang X, Liu J, Cheng Y, et al. (2014). Sirtuin-3 (SIRT3), a therapeutic target with oncogenic and tumor-suppressive function in cancer. Cell Death Dis. 2014; 5(2): 1047.
  8. Cheng Y, Ren X, Gowda AS, Shan Y, Zhang L, Yuan Y, et al. Interaction of Sirt3 with OGG1 contributes to repair of mitochondrial DNA and protects from apoptotic cell death under oxidative stress. Cell Death Dis. 2013; 4(7): 731.
  9. Bjørge MD, Hildrestrand GA, Scheffler K, Suganthan R, Rolseth V, Kuśnierczyk A, et al. Synergistic actions of Ogg1 and mutyh DNA glycosylases modulate anxiety-like behavior in mice. Cell rep. 2015; 13(12): 2671-2678.
  10. Bo H, Kang W, Jiang N, Wang X, Zhang Y, Ji LL. Exercise-induced neuroprotection of hippocampus in APP/PS1 transgenic mice via upregulation of mitochondrial 8-oxoguanine DNA glycosylase. Oxid Med Cell Longev. 2014; 2014:834502.
  11. Little J P, Safdar A, Wilkin G P, Tarnopolsky M A, Gibala M J. A practical model of low-volume high-intensity interval training induces mitochondrial biogenesis in human skeletal muscle: Potential mechanisms. J Physiol. 2010; 588(6): 1011–1022.
  12. Fathi A, Noorshahi M, Haghparast A, Hossini H. Effect of eight-week aerobic continuous and high intensity interval training on levels of SIRT3 in skeletal muscle tissue of Wistar rats. Physiol Exerc Phys Act. 2016; (16): 1277-1289. (Persian)
  13. Hodge T, Starnes J, Feger B, Hixson L, Harris MB. Effects of exercise and body temperature on eNOS, SIRT1, SIRT3 and Hsp70 expression in rat plantaris muscles (1164.6). The FASEB J. 2014; 28 (1): 1164-1166.
  14. Desquiret-Dumas V, Gueguen N, Leman G, Baron S, Nivet-Antoine V, Chupin S, et al. Resveratrol induces a mitochondrial complex I-dependent increase in NADH oxidation responsible for sirtuin activation in liver cells. J Biol Chem. 2013; 288(51): 36662-36675.
  15. Zhou X, Chen M, Zeng X, Yang J, Deng H, Yi L, et al. Resveratrol regulates mitochondrial reactive oxygen species homeostasis through Sirt3 signaling pathway in human vascular endothelial cells. Cell Death Dis. 2014; 5(12): e1576.
  16. Gertz M, Nguyen GT, Fischer F, Suenkel B, Schlicker C, Fränzel B, et al. A molecular mechanism for direct sirtuin activation by resveratrol. PloS one. 2012; 7(11): e49761.
  17. Chilibeck P, Bell G, Farrar R, Martin T. Higher mitochondrial fatty acid oxidation following intermittent versus continuous endurance exercise training. Can J Physiol Pharmacol. 1998; 76(9): 891-894.
  18. Lau YS, Patki G, Das‐Panja K, Le WD, Ahmad SO. Neuroprotective effects and mechanisms of exercise in a chronic mouse model of Parkinson’s disease with moderate neurodegeneration. Eur J Neurosci. 2011; 33(7), 1264-1274.
  19. Brandauer J, Andersen MA, Kellezi H, Risis S, Frøsig C, Vienberg SG, et al. AMP-activated protein kinase controls exercise training-and AICAR-induced increases in SIRT3 and MnSOD. Front Physiol. 2015; 6, 85.
  20. Ahn B-H, Kim H-S, Song S, Lee IH, Liu J, Vassilopoulos A, et al. A role for the mitochondrial deacetylase Sirt3 in regulating energy homeostasis. Proc Natl Acad Sci U S A. 2008; 105 (38): 14447-14452.
  21. Hokari F, Kawasaki E, Sakai A, Koshinaka K, Sakuma K, Kawanaka K. Muscle contractile activity regulates Sirt3 protein expression in rat skeletal muscles. J Appl Physiol . 2010; 109 (2): 332-340.
  22. Lanza IR, Short DK, Short KR, Raghavakaimal S, Basu R, Joyner MJ, et al. Endurance exercise as a countermeasure for aging. Diabetes. 2008; 57(11): 2933-2942.
  23. Radak Z, Toldy A, Szabo Z, Siamilis S, Nyakas C, Silye G, et al. The effects of training and detraining on memory, neurotrophins and oxidative stress markers in rat brain. Neurochem Int. 2006; 49(4), 387-392.
  24. Marfe G, Tafani M, Pucci B, Di Stefano C, Indelicato M, Andreoli A, et al. RETRACTED ARTICLE: The effect of marathon on mRNA expression of anti-apoptotic and pro-apoptotic proteins and sirtuins family in male recreational long-distance runners. BMC physiol. 2010; 10(1): 7.
  25. Steinbacher P, Eckl P. Impact of oxidative stress on exercising skeletal muscle. Biomolecules. 2015; 5(2), 356-377.
  26. Schirmer H, Pereira TCB, Rico EP, Rosemberg DB, Bonan CD, Bogo MR, et al. Modulatory effect of resveratrol on SIRT1, SIRT3, SIRT4, PGC1α and NAMPT gene expression profiles in wild-type adult zebrafish liver. Mol Biol Rep. 2012; 39(3): 3281-3289.
  27. Abdel-Wahab BA, Abdel-Wahab MM. Protective effect of resveratrol against chronic intermittent hypoxia-induced spatial memory deficits, hippocampal oxidative DNA damage and increased p47Phox NADPH oxidase expression in young rats. Behav Brain Res. 2016; 305: 65-75.
  28. Lodovici M, Bigagli E, Luceri C, Manni EM, Zaid M. Protective Effect of Resveratrol against Oxidation Stress Induced by 2-Nitropropane in Rat Liver. Pharmacology & Pharmacy (PP). 2011; 2: 127-135.
  29. Singh B, Shoulson R, Chatterjee A, Ronghe A, Bhat NK, Dim DC, et al. Resveratrol inhibits estrogen-induced breast carcinogenesis through induction of Nrf2-mediated protective pathways. Carcinogenesis. 2014; 35(8): 1872-1880.
  30. White AT, Schenk S. NAD+/NADH and skeletal muscle mitochondrial adaptations to exercise. Am J Physiol Endocrinol Metab. 2011; 303(3): E308-E321.
  31. Santos-Alves E, Marques-Aleixo I, Rizo-Roca D, Torrella J, Oliveira P, Magalhães J, et al. Exercise modulates liver cellular and mitochondrial proteins related to quality control signaling. Life Sci . 2015; 135: 124-130.

  • Receive Date 30 November 2018
  • Revise Date 07 February 2021
  • Accept Date 31 December 2020
  • First Publish Date 31 December 2020
  • Publish Date 22 August 2020