نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه فیزولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه آزاد اسلامی، واحد اصفهان (خوراسگان)، اصفهان، ایران،
2 استاد تمام، گروه فیزولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه آزاد اسلامی، واحد اصفهان (خوراسگان)، اصفهان، ایران
3 گروه فیزولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه آزاد اسلامی، واحد اصفهان
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Background and Objective: Obesity, as a chronic metabolic disorder, is associated with activation of inflammatory pathways, impaired energy regulation, and increased production of reactive oxygen species. Inflammation caused by obesity and exposure to acrylamide causes disturbances in the metabolic rate of liver tissue. In this study, the effect of the synergistic combination of enriched medium and cineole on the expression of key genes related to inflammation, metabolism, and oxidative stress in an obese animal model induced by a high-fat diet containing acrylamide was evaluated. Materials and Methods: 54 C57BL/6J mice were subjected to different treatments for eight weeks. Mice were randomly assigned to nine equal groups (each group containing 6 mice): (1) healthy control, (2) obese with a high-fat diet containing acrylamide (45% high-fat diet heated and enriched with 1000 ppm acrylamide), (3) obese with a high-fat diet without acrylamide (45% high-fat diet), (4) obese with an acrylamide diet and training in an enriched environment (The voluntary-exploratory physical activity protocol was implemented in an environment enriched with locomotor structures (ramps, platforms, ladders, carousels) and exploratory tools (chewable objects, tunnels) to assess the physical mobility and cognitive behaviors of rats in non-mandatory and low-stress conditions, eight weeks, five days/week, 60-90 minutes daily, low-moderate intensity), (5) obese with an acrylamide diet and cineole treatment (10 μM concentration by gavage), (6) obese with training in an enriched environment, (7) obese with cineole, (8) obese with an acrylamide diet, training in an enriched environment and cineole, (9) obese with an enriched environment and cineole (without acrylamide). Drug affinity results were based on pharmacophore modeling and molecular binding. Advanced biological data analysis was performed from microarray datasets. Gene expression was measured by Real-Time PCR from RNA extraction from liver tissue. Results: The use of the bioactive compound cineole in association studies improved the accuracy and computational efficiency and provides a potential strategic approach for designing protective agents that target PPARα-mediated metabolic pathways in liver tissue. Activity in enriched medium and cineole significantly increased the expression (P<0/001) of Sirt1, PPARα (P<0/001) and PPARγ (P<0/001) and significantly decreased (P<0/001) of CXCL15 and CYP2E1 (P<0/001) compared to other intervention groups. Conclusion: Analysis of biological data showed that the genes Sirt1, PPARα, PPARγ, CXCL15, and CYP2E1 play a key role in activating inflammatory pathways, disrupting energy regulation, and increasing the production of reactive oxygen species in the liver tissue of obese mice. The protective role of cineole and activity in an enriched environment modulates the expression of the mentioned genes. These two interventions can be considered as an effective approach in preventing metabolic disorders associated with obesity.
کلیدواژهها [English]
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