بررسی اثر متقابل اتوفاژی و آپوپتوز در بافت قلب موش‌های صحرایی دیابتی نوع دو در تعامل با تمرین هوازی و تزریق ویتامین د3

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه فیزیولوژی ورزشی، دانشکدۀ علوم انسانی و اجتماعی، دانشگاه کردستان، سنندج، ایران

2 گروه فیزیولوژی و فارماکولوژی، دانشکدۀ پزشکی، دانشگاه علوم پزشکی کردستان، سنندج، ایران

چکیده

زمینه و هدف: تعامل بین اتوفاژی و آپوپتوز در درک سلامت قلب، به‌ویژه در زمینۀ دیابت نوع دو، بسیار مهم است. هدف این تحقیق بررسی چگونگی تعدیل تمرین هوازی و مکمل ویتامین د3 در بافت قلب موش‌های صحرایی دیابتی است و راهبردهای درمانی بالقوه برای بهبود عملکرد قلب در شرایط دیابتی را برجسته می‌کند. بنابراین، در این پژوهش تأثیرات تمرین هوازی و تزریق ویتامین د3 بر اتوفاژی و آپوپتوز بافت قلب موش‌های صحرایی دیابتی بررسی شد.
مواد و روش‌ها: 40 سر موش نر نژاد ویستار با دامنۀ وزنی 200-240 گرم به دو گروه دریافت‌کنندۀ رژیم غذای استاندارد (هشت سر) و دریافت‌کنندۀ غذایی پرچرب به مدت شش هفته (32 سر) جایگزین شدند. پس از شش هفته، برای القای دیابت نوع دو، به مقدار 35 میلی‌گرم/کیلوگرم وزن بدن استرپتوزوتوسین (STZ) به‌صورت درون‌صفاقی تزریق شد. موش‌های صحرایی دریافت‌کنندۀ رژیم غذایی پرچرب به‌طور تصادفی در چهار گروه، کنترل دیابت (DC)، دیابت+ تمرین هوازی (DAT)، دیابت+ تزریق ویتامین د3 (DVD) و دیابت+ تمرین هوازی+ تزریق ویتامین د3 (DVDAT) جایگزین شدند. به مدت هشت هفته، سه جلسه/ هفته، تمرین هوازی با شدت 60 درصد حداکثر سرعت دویدن از طریق نوار گردان در گروه‌های تمرین هوازی انجام گرفت. گروه‌های دریافت‌کنندۀ ویتامین د3 محلول رقیق‌شدۀ ویتامین د3 با روغن کنجد با دوز 10000IU/kg به‌صورت هفتگی دریافت کردند. پس از اتمام دورۀ مداخلات تمرین هوازی و تزریق ویتامین د3 و بی‌هوش کردن رت‌ها، بافت قلب استخراج شد و سطوح پروتئینی Beclin-1، Bax و Bcl-2 بطن چپ بافت قلب به روش وسترن بلات اندازه‌گیری شد. تجزیه‌وتحلیل یافته‌ها با استفاده از آزمون تحلیل واریانس یکطرفه (آنووا) انجام گرفت.  
نتایج: بررسی داده‌ها نشان داد که دیابت نوع دو موجب افزایش سطوح Beclin-1، Bax و کاهش Bcl-2 شد (001/0>P). درحالی‌که تمرین هوازی، ویتامین د3 و تعامل هر دو موجب کاهش Beclin-1، Bax و افزایش Bcl-2 شد که این تغییرات در گروه تعاملی تمرین هوازی و ویتامین د3 بیشتر بود (001/0>P). نتایج همچنین نشان داد که سطوح گلوکز ناشتا و شاخص مقاومت به انسولین در گروه‌های DAT، DVD و DVDAT کاهش معناداری یافت (001/0>P) که این کاهش در گروه DVDAT بیشتر بود (001/0>P).
نتیجه‌گیری: در مجموع می‌توان گفت که دیابت نوع دو از طریق افزایش اتوفاژی بیش‌ازحد و آپوپتوز در بافت قلب موجب اختلال در هومئوستاز سلولی و سوخت‌وسازی از جمله مقاومت به انسولین می‌شود که این می‌تواند از طریق تمرین هوازی و ویتامین د3 معکوس شود و سوخت‌وساز گلوکز و مقاومت به انسولین بهبود یابد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Investigating the mutual effects of autophagy and apoptosis in heart tissue of type 2 diabetic rats in interaction with aerobic exercise training and vitamin D3 injection

نویسندگان [English]

  • Hadi Golpasandi 1
  • Mohammad Rahman Rahimi 1
  • Slahadin Ahmadi 2
1 Department of Exercise Physiology, Faculty of Humanities and Social Sciences, University of Kurdistan, Sanandaj, Iran
2 Department of Physiology and Pharmacology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
چکیده [English]

Background and Purpose: The interplay between autophagy and apoptosis is crucial in understanding heart health, particularly in the context of type 2 diabetes. The aim of this study was to investigate how aerobic training and vitamin D3 supplementation modulate the cardiac tissue of diabetic rats and highlight potential therapeutic strategies to improve cardiac function in diabetic conditions. Therefore, in this study, the effects of aerobic training and vitamin D3 injection on autophagy and apoptosis of heart tissue of diabetic rats were investigated.
Materials and Methods: Forty male Wistar rats with a weight range of 200 - 240 grams were divided into two groups receiving a standard diet (8 rats) and a high-fat diet (32 rats) for 6 weeks. After 6 weeks, in order to induce type 2 diabetes, 35 mg/kg body weight of streptozotocin (STZ) was injected intraperitoneally. Rats receiving high-fat diet were randomly divided into 4 groups, diabetes control (DC), diabetes + aerobic training (DAT), diabetes + vitamin D3 injection (DVD) and diabetes + aerobic training + vitamin D3 injection (DVDAT). The rats in the aerobic training group underwent aerobic training with an intensity of 60% of the maximum running speed through a treadmill for eight weeks, 3 sessions/week in the aerobic training groups. The groups receiving vitamin D3 received a diluted solution of vitamin D3 with sesame oil at a dose of 10000 IU/kg weekly. After the completion of aerobic training interventions and vitamin D3 injection and anesthetizing the mice, the heart tissue was extracted and the protein levels of Beclin -1, Bax and Bcl -2 in the left ventricle of the heart tissue were measured by western blot technique. The data were analyzed using one-way analysis of variance (ANOVA).
Results: Data analysis showed that type 2 diabetes increased the levels of Beclin-1, Bax and decreased Bcl-2 (P<0.001). Aerobic training, vitamin D3 and their interaction both decreased Beclin-1, Bax and increased Bcl -2, and that these changes were higher in the DVDAT group (P<0.001). The results also showed that fasting glucose levels and insulin resistance index decreased significantly in DAT, DVD and DVDAT groups (P<0.001), and this decrease was more in DVDAT group (P<0.001).
Conclusion: In general, it could be concluded that type 2 diabetes causes disturbances in cellular and metabolic homeostasis, including insulin resistance, through increased autophagy and apoptosis in heart tissue, which can be reversed through aerobic training and vitamin D3, and improve glucose metabolism and insulin resistance.

کلیدواژه‌ها [English]

  • Aerobic Exercise Training
  • Excessive Autophagy
  • Apoptosis
  • Bax
  • Beclin-1 and Diabetic Cardiomyopathy

مراجع

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  • تاریخ دریافت: 03 خرداد 1403
  • تاریخ بازنگری: 09 تیر 1403
  • تاریخ پذیرش: 10 تیر 1403
  • تاریخ اولین انتشار: 10 تیر 1403
  • تاریخ انتشار: 01 تیر 1403

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