نوع مقاله : مقاله پژوهشی
نویسندگان
گروه فیزیولوژی ورزش، دانشگاه آزاد اسلامی، واحد اصفهان (خوراسگان)، اصفهان، ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Background and Purpose: Liver disease is one of the main causes of death in diabetic patients. Type 2 diabetes is one of the most common chronic metabolic diseases and its prevalence is increasing rapidly all over the world. Liver is one of the most important organs that control blood glucose levels. Although the favorable role of Gymnema sylvester supplement and aerobic training has been reported in diabetic patients, the effect of Gymnema sylvester supplement and aerobic training on liver tissue is not well known. Therefore, the purpose of this research is the effect of Gymnema sylvester supplement on the signaling pathway involved in the oxidative stress of liver tissue in type 2 diabetic mice following aerobic training.
Materials and Methods: In this experimental research, 32 male C57BL/6 mice were randomly divided into 4 groups of eight including diabetes group and aerobic training, diabetes group and gymnema sylvester supplement, diabetes group and aerobic training with gymnema sylvester supplement and diabetes control group. Diabetic groups were made diabetic by high-fat diet for 20 weeks. Gymnema sylvester supplement groups were gavaged with 100 mg/kg daily for six weeks. Aerobic training groups performed exercise at a speed of 10 m/min on a treadmill for six weeks and five training sessions per week. Serum concentration of SOD and MDA by ELISA method and expression of Nrf2/Keap1 (nuclear factor erythroid 2–related factor 2/ Kelch-like ECH-associated protein 1) genes was measured by RT-PCR method. The data were analyzed by using SPSS and one-way analysis of variance, and Bonferroni's post-hoc test. The significance level was considered p≤0.05.
Results: Statistical analyses showed that six weeks of taking gymnema sylvester supplement, aerobic training and aerobic training with gymnema sylvester supplement caused a significant decrease in MDA and the expression of the Keap1 gene and a significant increase in SOD and the expression of the Nrf2 gene in the liver tissue (p<0.05). The greatest changes occurred in the diabetes group + aerobic training and gymnema sylvester supplement compared to diabetic control group (p<0.05). However, there were no significant differences between diabetic group and aerobic training group and gymnema supplement group (p<0.05).
Conclusion: Gymnema Sylvester supplement and aerobic training improved blood variables through reducing MDA and increasing SOD, as well as improving the signaling pathway involved in oxidative stress in liver tissue by increasing the expression of the Nrf2 gene and decreasing the expression of the Keap1 gene in type 2 diabetic mice.
کلیدواژهها [English]
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