تأثیر رژیم غذایی پرچرب و تمرینات مختلف بر بیان ژن‌های هورمون شبه‌متئورین و پروتئین شبه‌آنژیوپویتین 4 در بافت چربی موش‌های نر نژاد ویستار

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه تربیت بدنی و علوم ورزشی، واحد بجنورد، دانشگاه آزاد اسلامی، بجنورد، ایران

چکیده

زمینه و هدف: هورمون شبه‌متئورین (Metrnl) و پروتئین شبه‌آنژیوپویتین 4 (ANGPTL4)، از پروتئین‌های مؤثر در قهوه‌ای شدن بافت چربی هستند. هدف پژوهش حاضر بررسی تأثیر رژیم غذایی پرچرب و تمرینات ورزشی متعاقب آن بر عوامل درگیر در قهوه‌ای شدن بافت چربی در موش‌های نر بود.
مواد و روش‌ها: 30 سر موش صحرایی نر سالم نژاد ویستار به مدت هشت هفته از غذای پرچرب و شش سر دیگر (ND) از غذای معمولی تغذیه کردند. پس از هشت هفته، شش سر موش چاق‌شده با رژیم پرچرب (HFD) و شش سر موش تغذیه‌شده با رژیم غذایی معمولی (ND) قربانی  و مقایسه شدند. 24 سر موش چاق باقیمانده به چهار گروه شش‌تایی شامل کنترل (C)، تمرین مقاومتی (RT)، تمرین استقامتی (ET) و تمرین تناوبی هوازی (AIT) تقسیم شدند و به مدت هشت هفته، هفته‌ای پنج جلسه تمرین کردند. برای اندازه‌گیری بیان ژن هورمون شبه‌متئورین و پروتئین شبه‌آنژیوپویتین 4 از روش Real Time-PCR استفاده شد. از روش آماری تحلیل واریانس یکطرفه (آنووا) و آزمون تعقیبی توکی برای تعیین اختلاف بین گروه‌ها در سطح معناداری 05/0>P استفاده شد.
نتایج: افزایش معنادار بیان ژن Metrnl در گروه تمرین مقاومتی، استقامتی و تناوبی هوازی نسبت به گروه کنترل (001/0=P) و کاهش معنادار آن در بافت چربی موش‌های نر در گروه رژیم غذایی پرچرب نسبت به گروه رژیم غذایی معمولی دیده شد (001/0=P). همچنین کاهش معنادار بیان ژن ANGPTL4 در گروه تمرین مقاومتی، استقامتی و تناوبی هوازی نسبت به گروه کنترل (001/0=P) و افزایش معنادار بیان آن در بافت چربی موش‌های نر در گروه رژیم غذایی پرچرب نسبت به گروه رژیم غذایی معمولی گزارش شد (001/0=P). بین گروه‌های تمرینی تفاوت معناداری دیده نشد (05/0<P).
نتیجه‌گیری: با توجه به یافته‌های این پژوهش، گمان می‌رود تمرینات مقاومتی، استقامتی و تناوبی هوازی با افزایش هورمون شبه‌متئورین و کاهش پروتئین شبه‌آنژیوپویتین 4، راهکار مؤثری در افزایش بافت چربی قهوه‌ای و کاهش اختلالات مرتبط با اضافه وزن و چاقی باشد.
 

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of high-fat diet and different training programs on the expression of Meteorin- Like hormone and Angiopoietin-like protein 4 genes in adipose tissue of male Wistar rats

نویسندگان [English]

  • Sara Asghari
  • Sadegh Cheragh-Birjandi
  • Najmeh Rezaeian
  • Vahid Rezaei
Department of Physical Education, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran
چکیده [English]

Background and Purpose: Meteorin-like hormone (Metrnl) and Angiopoietin-like protein 4 (ANGPTL4) are effective proteins in fat tissue browning. The aim of the present study was to investigate the effect of high-fat diet and subsequent training on the factors involved in the browning of adipose tissue in male rats.
Materials and Methods: Thirty healthy male Wistar rats were fed high-fat diet (HFD)  and another six were fed normal diet (ND) for eight weeks. After eight weeks, six mice fed a HFD and six mice fed a ND were sacrificed and compared. The remaining 24 obese mice were divided into four groups of six including control (C), resistance training (RT), endurance training (ET) and aerobic interval training (AIT) and trained five times a week for eight weeks. Real Time-PCR method was used to measure the gene expression of Metrnl and ANGPTL4. One-way analysis of variance (ANOVA) and Tukey's post hoc test were used to determine between-group differences at a significance level of p<0.05.
Results: A significant increase in Metrnl gene expression was observed in the resistance, endurance and aerobic interval training groups compared to the control group (p=0.001) and a significant decrease in the adipose tissue of male mice in the HFD group compared to the ND group (p = 0.001). Moreover, a significant decrease in the expression of ANGPTL4 gene was found in the resistance, endurance, and aerobic interval training groups compared to the control group (p=0.001) and a significant increase in its expression in the fat tissue of male mice in the HFD group compared to the ND group (p = 0.001). No significant differences for both Metrnl and ANGPTL4 were observed between training groups (p> 0.05).
Conclusion: According to the results of the present study, it seems that resistance, endurance and aerobic interval training with increasing Metrnl and decreasing ANGPTL4 are effective in increasing brown fat tissue and reducing disorders related to overweight and obesity.
 

کلیدواژه‌ها [English]

  • Resistance training
  • Endurance training
  • Aerobic interval training
  • Obesity
  • Meteorin- Like hormone
  • Angiopoietin-like protein 4

مراجع

  1. Farajtabar Behrestaq S, Khonakdar M, Taghipour A, Askari B. The effect of aerobic training with royal jelly on the expression of BCL-2, Bax and Bax/BCL2 ratio in pancreatic tissue of obese rats. Daneshvar Medicine. 2024;31(6):26-36.
  2. Ezzeddin N. Socio-Economic and Environmental Factors Involved in Overweight and Obesity; A review Study. Health Res J. 2018;3(4):225-32.
  3. Ghesmati Z, Rashid M, Fayezi S, Gieseler F, Alizadeh E, Darabi M. An update on the secretory functions of brown, white, and beige adipose tissue: towards therapeutic applications. Reviews in Endocrine and Metabolic Disorders. 2024;25(2):279-308.
  4. Alizadeh H, Safarzade A, Talebi-Garakani E. The effect of interval training on serum meteorin-like hormone (metrnl) level in overweight adolescent boys. Journal of Applied Exercise Physiology. 2018;14(27):139-51.
  5. Nazari M, Minasian V, Hovsepian S. The effects of two types of interval training on the association between cardiovascular fitness and atherogenic biomarkers with blood pressure in Women with overweight/obesity. 2023.
  6. Dastah S, Babaei S. Effect of aquatic training on serum Fetuin-A, ANGPTL4 and FGF21 levels in type 2 diabetic obese women. Journal of Applied Health Studies in Sport Physiology. 2021;8(2):51-60.
  7. Tong Z, Peng J, Lan H, Sai W, Li Y, Xie J, et al. Cross-talk between ANGPTL4 gene SNP Rs1044250 and weight management is a risk factor of metabolic syndrome. Journal of Translational Medicine. 2021;19:1-12.
  8. Mendez-Gutierrez A, Osuna-Prieto FJ, Aguilera CM, Ruiz JR, Sanchez-Delgado G. Endocrine mechanisms connecting exercise to brown adipose tissue metabolism: a human perspective. Current Diabetes Reports. 2020;20(9):40.
  9. Mu W-J, Zhu J-Y, Chen M, Guo L. Exercise-mediated browning of white adipose tissue: its significance, mechanism and effectiveness. International Journal of Molecular Sciences. 2021;22(21):11512.
  10. Melo AB, Damiani APL, Coelho PM, de Assis ALEM, Nogueira BV, Ferreira LG, et al. Resistance training promotes reduction in Visceral Adiposity without improvements in Cardiomyocyte Contractility and Calcium handling in Obese Rats. International journal of medical sciences. 2020;17(12):1819.
  11. Rad RS, Fasihi-Ramandi E, Boldaji DK. The effect of 8-weeks of aerobic training and green tea supplementation on aerobic capacity, body mass index and fat percentage in obese and overweight officers students. Journal of Military Medicine. 2020;22:131-40.
  12. Hjinajaf S, Mohammadi F, Azizi M. Effect of aerobic interval exercise training on serum levels of 25-hydroxyvitamin D and indices anthropometry in overweight and obesity patients. Jundishapur Scientific Medical Journal. 2018;17(1):37-48.
  13. Dezhkam N, Rezaeian N. Effect of Six Weeks of Aerobic Training on Meteorin Like Factor Response and Insulin Resistance Index in Overweight and Obese Young Women. Journal of Applied Health Studies in Sport Physiology. 2021;8(1):28-35.
  14. Alizadeh H, Safarzadeh A, Talebi-Garakani E. Effect of resistance training on serum meteorin-like hormone level and insulin resistance index in overweight adolescent boys. Journal of Arak University of Medical Sciences. 2017;20(7):54-64.
  15. Kazemi-Esfahani S, Yaghoubi A. Changes in Angiopoietin-like 4 Protein Level and Insulin Resistance in Rats with Non-Alcoholic Fatty Liver Disease: Effect of High Intensity Interval Training and Portulaca Oleracea extract. Metabolism and Exercise. 2022;12(1):141-58.
  16. Sadeghi A, Gholami M, Matinhomaee H, Aabednatanzi H, Ghazalian F. Changes in the serum levels of ANGPTL3, ANGPTL4 and CRP following combined training alone or in combination with thyme ingestion in the obese men. Daneshvar Medicine. 2022;30(2):61-73.
  17. Sadeghi N, Rahmati S. The Effects of High-Intensity Interval Training on the Visceral Adipose Tissue Perilipin3 and Perilipin5 Genes Expression in Obese Male Rats with Non-Alcoholic Fatty Liver Disease. Journal of Mazandaran University of Medical Sciences. 2024;34(231):54-61.
  18. Jafarzadeh G, Zamani Behbahani A, Yaghmaei M, Salehi Veisi M. Comparison of the Effect of Running and Swimming on Some Liver Enzymes of Male Rats Induced by Doxorubicin. Journal of Isfahan Medical School. 2024;42(762):248-55.
  19. Dehghan F, Cheragh Birjandi S. Effect of Obesity on Skeletal Muscle Semaphorin 3C Levels in Male and Female Wistar Rats. Journal of North Khorasan University of Medical Sciences. 2022;14(3):92-9.
  20. Ghasemian SO, Seyyed A, Bazdar Teshnizi M, Mostajir M, Karimi-Dehkordi M. Effect of high-intensity interval training on cardiac oxidative stress parameters and blood serum biochemical profile in ovariectomized aged rats. Journal of Animal Research (Iranian Journal of Biology). 2023;36(3):226-39.
  21. Bakhtiyari A, Pournemati P. Effect 12 week high-intensity interval training and Endurance intensity training on proteins of AMPK and ERRα in elderly rats. medical journal of mashhad university of medical sciences. 2021;64(1):2308-18.
  22. Ghorbani Dasht Bayaz N, Donyaie A, Vosadi E. Comparing Endurance and Resistance training on the Expression of Senescence-Related Genes in the Visceral Adipose Tissue of obese rats. Journal of Sport Biosciences. 2023;15(3):37-49.
  23. Khalesi M. Effect of a period of swimming exercise on Sirt1 and FoxO3a genes expression in lung tissue of wistar rats. Journal of Sabzevar University of Medical Sciences. 2018;25(2):251-8.
  24. Javaid HMA, Sahar NE, ZhuGe D-L, Huh JY. Exercise inhibits NLRP3 inflammasome activation in obese mice via the anti-inflammatory effect of meteorin-like. Cells. 2021;10(12):3480.
  25. Lee JO, Byun WS, Kang MJ, Han JA, Moon J, Shin MJ, et al. The myokine meteorin‐like (metrnl) improves glucose tolerance in both skeletal muscle cells and mice by targeting AMPKα2. The FEBS Journal. 2020;287(10):2087-104.
  26. Bae JY. Aerobic exercise increases meteorin‐like protein in muscle and adipose tissue of chronic high‐fat diet‐induced obese mice. BioMed research international. 2018;2018(1):6283932.
  27. Li Z-Y, Song J, Zheng S-L, Fan M-B, Guan Y-F, Qu Y, et al. Adipocyte Metrnl antagonizes insulin resistance through PPARγ signaling. Diabetes. 2015;64(12):4011-22.
  28. Almasi Zefreei A, Taghian F, Jalali Dehkordi K. Effect of 8 weeks aerobic exercises and green coffee supplement on serum myonectin, meteorin-like, and insulin resistance in obese women. Journal of Mazandaran University of Medical Sciences. 2020;30(187):58-67.
  29. Bakhshi Z, Nayebifar S, Salehikia A, Nakhaei H. Effect of 8 weeks of continuous running on serum levels of Meteorine like protein and Interleukin-6 in male rats with syndrome metabolic. Journal of Practical Studies of Biosciences in Sport. 2021;9(19):78-89.
  30. Rao RR, Long JZ, White JP, Svensson KJ, Lou J, Lokurkar I, et al. Meteorin-like is a hormone that regulates immune-adipose interactions to increase beige fat thermogenesis. Cell. 2014;157(6):1279-91.
  31. Xu A, Lam MC, Chan KW, Wang Y, Zhang J, Hoo RL, et al. Angiopoietin-like protein 4 decreases blood glucose and improves glucose tolerance but induces hyperlipidemia and hepatic steatosis in mice. Proceedings of the National Academy of Sciences. 2005;102(17):6086-91.
  32. Seo DY, Park SH, Marquez J, Kwak H-B, Kim TN, Bae JH, et al. Hepatokines as a molecular transducer of exercise. Journal of Clinical Medicine. 2021;10(3):385.
  33. Lichtenstein L, Berbée JF, van Dijk SJ, van Dijk KW, Bensadoun A, Kema IP, et al. Angptl4 upregulates cholesterol synthesis in liver via inhibition of LPL-and HL-dependent hepatic cholesterol uptake. Arteriosclerosis, thrombosis, and vascular biology. 2007;27(11):2420-7.
  34. Janssen AW, Katiraei S, Bartosinska B, Eberhard D, Willems van Dijk K, Kersten S. Loss of angiopoietin-like 4 (ANGPTL4) in mice with diet-induced obesity uncouples visceral obesity from glucose intolerance partly via the gut microbiota. Diabetologia. 2018;61:1447-58.
  35. Cullberg KB, Christiansen T, Paulsen S, Bruun J, Pedersen S, Richelsen B. Effect of weight loss and exercise on angiogenic factors in the circulation and in adipose tissue in obese subjects. Obesity. 2013;21(3):454-60.
  36. Soori R, Khosravi N, Mirshafiey SA, Gholijani F, Rezaeian N. Effects of Resistance Training on Angiopoietin-Like Protein 4 and Lipids Profile Levels in Postmenopausal Obese Women. Sport Physiology. 2018;9(36):39-58.
  37. Arefi Shirvan R, Ghorbanian B, Ghorbanzadeh B. The effect of 12 weeks of aerobic exercise on the serum levels of angiopoietin-like protein 4 (ANGPTL4) and beta-aminoisobutyric acid (BAIBA) in men with metabolic syndrome. Journal of Sport Biosciences. 2022;14(4).
  38. Rahmani E, Akbarzadeh S, Nazari A, Motamed N, Ghodrati S, Abasi Fard A, et al. Serum levels of angiopoietin-like protein 4 in polycystic ovary syndrome women with normal body mass index. The Iranian Journal of Obstetrics, Gynecology and Infertility. 2018;21(2):1-8.
  39. Karami M, Ghafari M, Banitalebi E. Comparison of the Effect of Two Personalized Low Volume-High Intensity and Combined (Strength-Aerobic) Exercises on Angiopoietin-like Protein 4 (ANGPTL4) Serum Levels in Women with Type 2 Diabetes: A Short Report. Journal of Rafsanjan University of Medical Sciences. 2020;19(1):97-106.
  40. Chang H, Kwon O, Shin M-S, Kang GM, Leem YH, Lee CH, et al. Role of Angptl4/Fiaf in exercise-induced skeletal muscle AMPK activation. Journal of Applied Physiology. 2018;125(3):715-22.
  41. Kavosi H, Cheragh-Birjandi S, Rezaeian N, Yaghoubi A. The effect of eight weeks of intense endurance, resistance and high intensity interval training on SLIT-2 and UCP-1 gene expression in obese male Wistar rats. Journal of Practical Studies of Biosciences in Sport. 2023.
  42. Zhang H, Tong TK, Qiu W, Zhang X, Zhou S, Liu Y, et al. Comparable effects of high‐intensity interval training and prolonged continuous exercise training on abdominal visceral fat reduction in obese young women. Journal of diabetes research. 2017;2017(1):5071740.

 

فایل ها

سابقه مقاله

  • تاریخ دریافت: 02 تیر 1403
  • تاریخ بازنگری: 02 مهر 1403
  • تاریخ پذیرش: 03 مهر 1403
  • تاریخ اولین انتشار: 03 مهر 1403
  • تاریخ انتشار: 01 آبان 1403

هم رسانی

ارجاع به این مقاله

آمار