نوع مقاله : مقاله پژوهشی
نویسندگان
گروه فیزیولوژی ورزش، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Background and Purpose: Demyelination, axonal damage and loss of neurons associated with aging and advancing senescence, which lead to decreased cognitive and motor function and neurological diseasesare among the concerns of today's societies due to the global increase in the elderly population. Exercise, not only improves overall brain health, it also acts as a powerful anti-aging treatment with minimal side effects. In addition, ursolic acid with its anti-inflammatory properties prevents brain damage and reduces cognitive defects. Therefore, the aim of this study is to investigate the effect of simultaneous administration of ursolic acid supplement along with regular exercise in an enriched environment on the expression of genes involved in myelination including myelin oligodendrocyte glycoprotein (MOG), myelin proteolipid protein (PLP), myelin basic protein (MBP) and Myelin-associated glycoprotein (MAG) in aged rats.
Materials and Methods: In this experimental study, 20 male Wistar rats, aged 16-20 months, were divided into four equal groups including: 1) control + placebo, 2) supplement, 3) exercise and 4) supplement + exercise. Regular exercise was performed for 4 weeks, 5 sessions per week and 30 minutes per session, which included voluntary exercise in an enriched environment (a metal cage larger than a standard cage with structures for the animal to climb, opportunities for shelter (houses, PVC pipes, cardboard tunnels), chews, spinning wheel and a set of 14 different non-chewable plastic objects). Nanoliposomal ursolic acid supplement gavage was performed at the rate of 250 mg/kg body weight on an empty stomach and according to training days. The expression of MOG, PLP, MBP and MAG genes in cerebellar tissue was measured by Real-Time PCR method. The data were analyzed using two-factor analysis of variance and Bonferroni's post-hoc test at a significance level of P≤0.05.
Results: The simultaneous administration of ursolic acid and performing regular exercise caused a significant increase in the expression of all studied genes compared to the other three groups. In addition, voluntary regular exercise and ursolic supplementation alone led to a significant increase in the expression of MOG, PLP and MAG genes compared to the old control group.
Conclusions: According to the research findings, the simultaneous administration of ursolic acid supplement and regular physical exercise in old age, may reduce axonal damage, neuronal destruction and age-related cognitive function decline through stimulating myelination. However, due to the limited research in this field, more investigations are needed in human.
کلیدواژهها [English]
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