تأثیر حاد فعالیت استقامتی شدید و متوسط بر غلظت سرمی CC16، SP-D و نسبت CC16/SP-D در مردان بزرگسال سالم فعال

پورمناف, هادی; نیکوخصلت, سعید; ساری صراف, وحید; امیرساسان, رامین

doi:10.48308/joeppa.2025.241338.1391

تأثیر حاد فعالیت استقامتی شدید و متوسط بر غلظت سرمی CC16، SP-D و نسبت CC16/SP-D در مردان بزرگسال سالم فعال

نوع مقاله : مقاله پژوهشی

نویسندگان

هادی پورمناف

سعید نیکوخصلت

وحید ساری صراف

رامین امیرساسان

گروه فیزیولوژی ورزشی، دانشکدة تربیت بدنی و علوم ورزشی، دانشگاه تبریز، تبریز، ایران

10.48308/joeppa.2025.241338.1391

چکیده

زمینه و هدف: یافته‌های پژوهشی پیشین نشان می‌دهند که افزایش میزان تهویه در طول فعالیت ورزشی استقامتی سبب تبخیر مایع سطح سلول‌های اپی‌تلیال مسیر هوایی می‌شود و دستگاه تنفسی را تحت فشار برشی قرار می‌دهد. این شرایط سبب کاهش یکپارچگی و آسیب سلول‌های اپی‌تلیال می‌شود. با این همه، تأثیر شدت فعالیت استقامتی بر نشانگرهای آسیب اپی‌تلیال مسیر هوایی از جمله پروتئین‌های سلول کلارا (CC16)، پروتئین سورفکتنت D (SP-D) و نسبت CC16/SP-D تاکنون ناشناخته مانده است. بنابراین، این پژوهش با هدف بررسی تأثیر حاد فعالیت استقامتی شدید و متوسط بر CC16، SP-D و نسبت CC16/SP-D در مردان بزرگسال سالم فعال انجام گرفت.
مواد و روش‌ها: 20 مرد بزرگسال سالم فعال (سن 3 ± 22 سال؛ وزن 8 ± 75 کیلوگرم؛ قد 06/0 ± 82/1 متر؛ BMI 86/1 ± 23 کیلوگرم بر متر مربع) به‌صورت تصادفی به دو گروه فعالیت استقامتی شدید (HE، 10 نفر) و متوسط (ME، 10 نفر) تقسیم شدند. آزمودنی‌های هر دو گروه یک جلسه فعالیت استقامتی مداوم را به مدت 20 دقیقه روی نوار گردان انجام دادند. شدت تمرین برای گروه HE و ME به‌ترتیب 85–90 درصد و 65–70 درصد ضربان قلب بیشینه بود. سرعت اولیة نوار گردان برای گروه‌های HE و ME به‌ترتیب 9 و 5/7 کیلومتر در ساعت تنظیم شده و در طول فعالیت، سرعت نوار گردان بر پایة شدت تعیین‌شده برای هر فرد به‌طور متناسب تغییر داده شد. برای تجزیه‌وتحلیل سطوح سرمی CC16 و SP-D، نمونه‌های خونی (پنج میلی‌لیتر) آزمودنی‌ها با استفاده از روش استاندارد از ورید پیش‌آرنجی، پیش (مقدار پایه) و یک ساعت پس از اتمام مداخله توسط متخصص مجرب آزمایشگاه جمع‌آوری شد. برای مقایسة بین‌گروهی سن، ویژگی‌های آنتروپومتریکی (وزن، قد و BMI)، تودة بدون چربی، مقدار ماهیچة بدن و مسافت پیمود‌ه‌شده از آزمون تی مستقل (Independent T-test) و برای مقایسة بین‌گروهی متغیرها، از آزمون تحلیل کوواریانس(ANCOVA) استفاده شد. برای مقایسة درون‌گروهی نیز آزمون تی وابسته (Paired samples T-test) به‌کار گرفته شد.
نتایج: بر پایة یافته‌های درون‌گروهی، سطح سرمی CC16 (008/0 = P) و نسبت CC16/SP-D (006/0 = P) پس از فعالیت استقامتی شدید به‌طور معناداری افزایش یافت، اما در مقدار SP-D پس از مداخله در مقایسه با مقدار پایه تفاوت معناداری دیده نشد (064/0 = P). همچنین پس از مداخله در مقدار CC16 (759/0 = P)، SP-D (072/0 = P) و نسبت CC16/SP-D (121/0 = P) در گروه ME دیده نشد .تفاوت بین‌گروهی CC16 (021/0 = P) و نسبت CC16/SP-D (025/0 = P) معنادار بود، اما تفاوت SP-D (446/0 = P) پس از مداخله معنادار نبود.
نتیجه‌گیری: در این پژوهش برای نخستین بار نشان داده شد که فعالیت استقامتی شدید سبب رهایش CC16 و افزایش نسبت CC16/SP-D شد، اما تأثیری بر رهایش SP-D پس از مداخله نداشت. همچنین فعالیت استقامتی با شدت متوسط تأثیری بر رهایش نشانگرهای یکپارچگی اپی‌تلیال مسیر هوایی نداشت.

کلیدواژه‌ها

پروتئین‌های ضدالتهابی

پروتئین سلول کلارا (CC16)

پروتئین سورفکتنتD (SP-D)

یکپارچگی اپی‌تلیال مسیر هوایی

موضوعات

قلب و عروق و گردش خون

عنوان مقاله English

The acute effect of high- and moderate-intensity endurance exercise on serum concentrations of club cell protein 16 (CC16), surfactant protein D (SP-D), and the CC16/SP-D ratio in active healthy adult men

نویسندگان English

Hadi Pourmanaf

Saeid Nikoukheslat

Vahid Sari Sarraf

Ramin Amirsasan

Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tabriz, Tabriz, Iran

چکیده English

Background and Purpose: Previous research has shown that increased ventilation during endurance exercise promotes evaporation of the airway epithelial lining fluid and imposes mechanical shear stress on the respiratory system. These physiological responses may compromise airway epithelial integrity and contribute to epithelial cell injury. Nevertheless, the effect of endurance exercise intensity on biomarkers of airway epithelial injury, including club cell protein 16 (CC16), surfactant protein D (SP-D), and the CC16/SP-D ratio, remains unclear. Therefore, the present study was conducted to investigate the acute effects of high- and moderate-intensity endurance exercise on serum CC16, SP-D, and the CC16/SP-D ratio in healthy, physically active adult men.
Materials and Methods: Twenty healthy, physically active adult men (Mean±SD; age, 22±3 years; weight, 75±8 kg; height, 182±6 cm; BMI, 23±1.86 kg/m2) were randomly assigned to a high-intensity endurance group (HE, n = 10) or a moderate-intensity endurance group (ME, n = 10). Participants in both groups completed a single 20-minute continuous treadmill running session. Exercise intensity was prescribed at 85–90% of maximal heart rate (MHR) for the HE group and 65–70% of MHR for the ME group. The initial treadmill speed was set at 9 km/h for the HE group and 7.5 km/h for the ME group and was adjusted throughout the exercise session to maintain the prescribed target intensity for each participant. To determine serum CC16 and SP-D concentrations, 5-mL venous blood samples were collected from the antecubital vein by an experienced laboratory technician using standard procedures at baseline (pre-exercise) and one hour after completion of the exercise session. Analysis of covariance (ANCOVA) was performed to compare post-exercise outcomes between groups, whereas paired-samples t-tests were used to assess within-group changes.
Results: Based on the within-group analyses, serum CC16 (p=0.008) and the CC16/SP-D ratio (p=0.006) increased significantly following high-intensity endurance exercise, whereas no significant change was observed in SP-D concentrations compared with baseline (p=0.064). In contrast, no significant changes were detected in serum CC16 (p= 0.759), SP-D (p=0.072), or the CC16/SP-D ratio (p=0.121) following moderate-intensity endurance exercise. Between-group comparisons revealed significant differences in post-exercise CC16 concentrations (p=0.021) and the CC16/SP-D ratio (p=0.025), whereas no significant between-group difference was observed in SP-D concentrations (p= 0.446).
Conclusion: The findings of the present study demonstrated for the first time that high-intensity endurance exercise induces more pronounced increases in CC16 release and the CC16/SP-D ratio than moderate-intensity endurance exercise, while it did not affect SP-D release.

کلیدواژه‌ها English

Airway epithelial integrity

Anti-inflammatory proteins

Clara cell protein (CC16)

Surfactant protein D (SP-D)

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