نوع مقاله : علمی - پژوهشی
نویسندگان
1 1-کارشناسی ارشد، گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه کاشان، کاشان، ایران
2 2-استادیار، گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه کاشان، کاشان، ایران
3 استادیار، گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه کاشان، کاشان، ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Background and Objective: The Western diet leads to a reduction in antioxidant enzymes in heart tissue, resulting in adverse effects such as inflammation and oxidative stress, followed by cardiovascular abnormalities. Therefore, the aim of this study was to investigate the effect of exercise training on cardiac antioxidant markers and oxidative stress in mice fed a western diet.
Materials and Methods: In this study, male C57BL/6J mice were divided into three groups: control (CON), western diet (WD), and a combined group of interval training plus western diet (WD+HIIT). The CON group received a standard diet for 4 weeks consisting of 10% energy from fat, 67% from carbohydrates, and 23% from protein. The diet of the WD and WD+HIIT groups consisted of 20% protein, 35% carbohydrates, and 45% fat. Sugar (50 g/L) containing 55% fructose and 45% sucrose was added to the drinking water of these groups. In addition to receiving the western diet, the WD+HIIT group performed HIIT exercises. Initially, a graded running test on a treadmill was conducted before the start of the designed interventions, and maximal speed (Vmax) was measured. Then, after 4 weeks of receiving the western diet, the mice performed training for 4 weeks, 3 sessions per week, at an intensity of 85–90% of maximal speed, with 2-minute active rest periods at 50% of maximal speed. Training speed progressively increased from 19 m/min in the first week to 22 m/min in the fourth week, and active rest period speed increased from 8 m/min in the first week to 11 m/min in the fourth week. At the end of 8 weeks, the mice were anesthetized by intraperitoneal injection of a combination of xylazine (10 mg/kg) and ketamine (75 mg/kg), and heart tissue was collected, washed, and stored in a freezer at -70°C until analysis.
Results: In the group fed a western diet, the levels of SOD, CAT, and TAC markers showed a significant decrease compared to the control group, while MDA levels increased (P<0.05). HIIT training led to a significant increase in antioxidant markers compared to both the western diet and control groups, and showed a significant decrease in MDA (P<0.05).
Conclusion: It appears that a western diet, through inhibiting SOD and CAT enzymes and reducing total antioxidant capacity, provides the basis for increased lipid peroxidation (MDA) in the heart. In contrast, HIIT training, by restoring and even enhancing these antioxidant markers beyond baseline levels, inhibits oxidative stress. These data support the hypothesis of exercise cross-adaptation against nutritional injuries and suggest the application of HIIT in preventing cardiovascular abnormalities induced by a western lifestyle.
کلیدواژهها [English]
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