تاثیر هشت هفته تمرین هوازی تداومی و تناوبی شدید بر مقادیر VEGF-A و VEGFR-2 بافت مغز موش‌های صحرایی نر ویستار

نوع مقاله : علمی - پژوهشی


1 داﻧﺸﮕﺎه ﺷﻬﯿﺪﺑﻬﺸتی

2 داﻧﺸﮕﺎه ﻋﻠﻮم ﭘﺰﺷﮑﯽ ﺷﻬﯿﺪ ﺑﻬﺸﺘﯽ


هدف: تحقیق حاضر، بررسی تاثیر هشت هفته تمرین استقامتی در مقایسه با تمرین تناوبی با شدت بالا بر مقادیر VEGF-A و VEGFR2 در سه ناحیه استراتوم، هیپوکامپ و قشر بافت مغز موش صحرایی نر ویستار بود. روش­شناسی: بدین منظور از 24 سر موش نر نژاد ویستار (9 هفته،20±200 گرم) استفاده شد. موش­ها پس از یک هفته آشنایی با محیط آزمایشگاه به روش تصادفی به سه گروه: کنترل (Cont)؛ تمرین تداومی استقامتی؛ تمرین تناوبی با شدت بالا (HIIT) تقسیم و پروتکل هشت هفته­ای تمرین تداومی و تناوبی را انجام دادند. 24 ساعت پس از آخرین جلسه تمرین حیوانات کشته و نمونه­گیری در سه ناحیه استراتوم، هیپوکامپ و قشر بافت مغز انجام گرفت. میزان پروتئین VEGF-A، VEGFR2 از طریق روش وسترن بلات انجام شد. برای بررسی اختلاف معناداری در مقادیر VEGF-A و VEGFR2در سه گروه از آنالیز واریانس یکطرفه  استفاده شد. یافته­ها: نتایج نشان داد که مقادیر پروتئین VEGF-A در ناحیه استراتوم در گروه HIIT، در ناحیه هیپوکامپ در گروه تمرین تداومی استقامتی و در ناحیه قشر هر در گروه HIIT بیش از گروه کنترل بود (5/0>P). همچنین مقادیر پروتئین VEGFR2 در دو ناحیه استراتوم و هیپوکامپ در هر دو گروه بیش از گروه کنترل بود. در حالی که در ناحیه قشر این تفاوت معنی­دار نبود. نتیجه­گیری: یافته­های پژوهش حاضر نشان داد که هر دو روش تمرین تداومی استقامتی و تناوبی با شدت بالا می تواند باعث افزایش مقادیر پروتئین­های VEGF-A و VEGFR2 گردد . با این حال ورزش تناوبی با شدت بالا به علت مزیت زمانی می­تواند جایگزینی مناسب برای تمرین تداومی استقامتی باشد.


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