تاثیر هشت هفته تمرین هوازی تداومی و تناوبی شدید بر سطوح SIRT3 بافت عضله اسکلتی موش های صحرایی چاق ویستار

نوع مقاله : علمی - پژوهشی

نویسندگان

چکیده

هدف: سیرت 3 یکی از اعضای خانواده پروتئین دی استیلازهای سیرتوئین است که در میتوکندری قرار دارد و عملکرد میتوکندری را تنظیم می کند. هدف از مطالعه حاضر بررسی تاثیر هشت هفته تمرین هوازی تداومی و تناوبی شدید بر سطوح SIRT3 بافت عضله اسکلتی موش صحرایی چاق ویستار بود. روش پژوهش: 24 موش صحرایی بطور تصادفی به چهار گروه 6 تایی: 1) چاق- تمرین تناوبی (HIIT)؛ 2) چاق- تمرین تداومی (CT)؛ 3) کنترل چاق (OB) و 4) کنترل غیرچاق (Cont.) تقسیم شدند. طی دوره تحقیق به موش­های گروه های 1، 2 و 3 غذای پرچرب داده شد. پس از آشناسازی، موش های گروه CT و HIIT به مدت هشت هفته سه جلسه در هفته به ترتیب تمرین تداومی هوازی و تمرین تناوبی با شدت بالا را انجام دادند. 48 ساعت پس از آخرین جلسه تمرینی، موش­ها تشریح شدند. نتایج: بررسی وسترن بلاتینگ نشان داد که میزان سیرت 3 عضله نعلی در گروه های HIIT و CT نسبت به گروه های OB و Cont. بطور معنی داری بیشتر بود (05/0< P). همچنین میزان سیرت 3 گروه HIIT نسبت به گروه CT بیشتر بود که البته معنی دار نبود. همچنین تفاوت معنی داری بین میزان سیرت 3 گروه OB و Cont. مشاهده نشد؛ با این‌حال میزان سیرت 3 در گروه OB کمتر بود. نتیجه­گیری: به نظر می‌رسد استفاده از تمرینات HIIT می تواند به اندازه تمرینات تداومی بر عملکرد میتوکندری بافت عضلانی و در نهایت بر طول عمر بویژه در افراد چاق موثر باشند و باعث افزایش فاکتورهای مهمی همچون سیرت 3 شوند.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of eight-week aerobic continuous and high intensity interval training on levels of SIRT3 in skeletal muscle tissue of Wistar rats

چکیده [English]



Purpose: SIRT3 is a member of the sirtuin family of protein deacetylases that is localized in mitochondria
and regulates mitochondrial function. The aim of this study was to investigate the effect of eight-week
aerobic continuous and high intensity interval training on levels of SIRT3 in Wistar rat’s skeletal muscle
tissue. Methods: 24 Wistar rats were randomly divided into four groups of six animals each, namely: 1)
Obese- High-intensity interval training (HIIT), 2) Obese- Continues training (CT) 3) Obese control (OB) and
4) Non-Obese control (Cont.). During the study, groups of rat 1, 2 and 3 were given a high-fat diet. After
familiarization, CT and HIIT rats performed aerobic continuous training and high intensity interval training
three times a week for eight weeks, respectively. 48 hours after the last training session, the rats were
sacrificed. Results: Western blot analysis showed that the amount of SIRT3 protein of Soleus muscle in HIIT
and CT groups was higher than OB and Cont. groups significantly (p< 0.05). Also, SIRT3 content was higher
in HIIT than CT group, though insignificant. Also, no significant difference was observed between SIRT3
content of OB and Cont. However, SIRT3 content was lower in OB group. Conclusion: It seems that using
HIIT exercise can be as effective as continuous training on muscle mitochondrial function and ultimately
contribute to longevity, especially in obese patients and raises important factors such as SIRT3.

کلیدواژه‌ها [English]

  • Sirtuins
  • Obesity
  • Interval training
  • Life span

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سابقه مقاله

  • تاریخ دریافت: 30 خرداد 1395
  • تاریخ بازنگری: 22 خرداد 1403
  • تاریخ پذیرش: 11 دی 1399
  • تاریخ اولین انتشار: 11 دی 1399
  • تاریخ انتشار: 01 آذر 1394

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